Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells

Abstract

The aim of the present study was to examine the effect of fatty acid binding protein-5 (FABP-5) gene on the proliferation, apoptosis and invasion of human gastric cancer SGC-7901 cells. The viability, apoptosis and cell invasion of SGC-7901 cells before and after FABP5 knockdown were taken as the study objects, design and synthesis of siRNA interference sequence were conducted according to FABP-5 mRNA coding sequences, and SGC-7901 cells were transiently transfected. The human gastric cancer SGC-7901 cells were divided into three groups: FABP-5 siRNA group, negative control group and blank control group. FABP-5 gene mRNA and protein expression levels were detected by RT-PCR and western blot analysis. The CCK-8 assay was used to detect in vitro cell proliferation, flow cytometry (FCM) was used to detect changes in cell cycle and apoptosis in each group, TUNEL staining was used to detect apoptosis in each group, and the cell invasion chamber assay was used to detect cell invasiveness in each group. Each test was repeated three times. The results of the RT-PCR and western blot analysis showed that, expression of FABP-5 mRNA and protein in the FABP-5 siRNA group was significantly decreased compared with the negative and blank control groups. The cell growth rate in the FABP-5 siRNA group was significantly retarded, cell cycle was arrested in G0/G1 phase, the number of cells in S phase was reduced, and compared with the negative and blank control groups, the apoptotic rate in the FABP-5 siRNA group was significantly increased (P<0.01), while proliferation and invasiveness were significantly decreased (P<0.05). In conclusion, specific FABP-5 gene silencing may reduce the invasiveness of gastric cancer cells, inhibit cell proliferation, and arrest cell cycle in G0/G1 phase, resulting in a significant increase in apoptosis.

Exploration of prognostic index based on immune-related genes in patients with liver hepatocellular carcinoma

“…Similar results have been obtained in human cells. The silence of FABP5 gene made effects on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells [29]. Above results from previous studies supported that the IRGs screened with our method showed clinical significance.…”

FABP5 regulates the proliferation of clear cell renal cell carcinoma cells via the PI3K/AKT signaling pathway

“…Recently, FABP5, which promotes tumor cell growth in cervical cancer, was identified as a potential biomarker for lymph node metastasis ( 18 , 19 ). FABP5 gene silencing inhibited the proliferation and invasion of human SGC-7901 gastric cancer cells in vitro ( 20 ), and FABP5 stimulated hepatocellular carcinoma progression and metastasis via EMT ( 21 ). Considering the pivotal functions of the PI3K/AKT signaling pathway in tumor cells, particularly ccRCC cells, we hypothesized that FABP5 may affect ccRCC cell function via the PI3K/AKT signaling pathway.…”

Berberine Suppressed Tumor Growth through Regulating Fatty Acid Metabolism and Triggering Cell Apoptosis via Targeting FABPs

“…Studies have shown that the upregulation of FABP5 stimulates fatty acid transport, which finally induces cell growth and survival [44]. It was also presented that the silence of the FABP5 gene might attenuate the invasiveness of gastric cancer cells, detain cell proliferation, and arrest the cell cycle in the G0/ G1 phase, leading to a significant increase in apoptosis [45]. BMS309403 is a well-designed inhibitor of FABPs (FABP3-5, and −7), which interacts with the fatty acid-binding pocket to inhibit the binding of fatty acids [23,46].…”