A Box-Behnken design was used to study the effects of feed moisture content (18-20%), barrel temperature (115-135°C) and screw speed (225-275 rpm) on the responses; antioxidant activity, total phenolics, dietary fibre, colour (L-value), hardness, expansion ratio, water absorption index and overall acceptability for development of gluten-free extrudate based on germinated Chenopodium album flour. Second-order polynomial fitted model showed that temperature most significantly (P ≤ 0.05) affected the antioxidant activity (AoxA), total phenolics (TPC), expansion ratio, hardness and overall acceptability (OA). Antioxidant activity, total phenolic content and dietary fibre of extrudate varied from 11.56 to 15.93%, 56.77 to 81.28 mg/100 g and 18.65 to 22.