Effect of Extraction Solvents on Total Polyphenolic Content and Antioxidant Capacity of Syzygium Aromaticum L. Flower Bud from Ethiopia

Abstract

In this study, the antioxidative activity and polyphenolic content of Syzygium aromaticum’s flower bud were compared under different extraction solvents including chloroform, ethyl acetate, methanol, and aqueous. The antioxidant activity was assessed via established in vitro assay models such as 2, 2-diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging assay, NO- radical scavenging assay, H2O2 scavenging assay and Fe3+ reducing capacity. Total phenolic content was measured according to Folin–Ciocalteu’s method, and total flavonoid content was estimated by using the aluminum chloride colorimetric method. The results showed that aqueous extract possessed the highest TPC ( 19.11 ± 2.76 mg GAE/g DW) and TFC ( 15.32 ± 1.53 mg CtE/g DW). Among the extracts, methanol extract exerted the strongest radical DPPH quenching activity with an IC50 value of 303.56 ± 13.14 μg/mL. The highest NO- radical scavenging activity was shown by methanol extract (IC50 192.94 ± 1.9 μg/mL) which is stronger than BHT (IC50 247.64 ± 12.89 μg/mL). Methanol extract showed a strong H2O2 scavenging activity (IC50 233.71 ± 3.72 μg/mL). The highest Fe3+ reducing capacity was shown by methanol extract ( Absorbance = 0.36 ± 0.05 ). Strong and positive correlations were observed between total phenolic and flavonoid contents and the antioxidant assays. The results of the present work revealed that the tested spice demonstrated high antioxidant activity, total phenolics, and flavonoids. Thus, this spice is worth considering as important source of natural antioxidant agents.

Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study

“…Our results showed that the aqueous extracts had significantly higher free radical scavenging activity than the pure solvent extracts. According to Babbar et al (2014) andTemesgen et al (2022), the differences in antioxidant capacity may be due to the quantity and quality of phenolic compounds present in each solvent. Phenolic compounds exhibit antioxidant activity by donating hydrogen atoms to free radicals and scavenging other reactive species, such as OH • , NO 2 • , N 2 O 3 , ONOOH, and HOCl.…”

“…Our results showed that the aqueous extracts had significantly higher free radical scavenging activity than the pure solvent extracts. According to Babbar et al ( 2014 ) and Temesgen et al ( 2022 ), the differences in antioxidant capacity may be due to the quantity and quality of phenolic compounds present in each solvent.…”

Nanophytosomal Gel of Heydotis corymbosa (L.) Extract against Psoriasis: Characterisation, In Vitro and In Vivo Biological Activity

“…The total flavonoid concentration of the extracts was measured using a colourimetric technique based on aluminium chloride [ 53 ], using a quercetin standard curve, and the findings are given as mg QeE/g Extract DW. The calibration curve of quercetin standard solutions in methanol was prepared at 376.0 nm using UV spectrophotometer (Shimadzu UV-1800, Shimadzu Analytical, Mumbai, India) covering the concentration range of 0.2 to 1.0 mg/mL, r 2 = 0.9675.…”

Determination of Polycyclic Aromatic Hydrocarbon Content in Garden Herbal Plants Using Liquid Chromatographic Analysis (HPLC-FL)

“…Flavonoid glycosides, phenolic acids and polyphenols of strong antioxidant activity are also present in the aerial parts of mint plants and can be extracted with a range of solvents. Among the phenolics, the highest contents were reported for narirutin, narigenin-7-oglucoside, luteolin-7-o-rutinoside, luteolin-diglucoronide, hesperidin, isorhoifolin, eriocitrin; rosmaric, cinnamic and caffeic acids [ 24 , 25 ].…”