Effect of extraction methods on the preparation of electrospun/electrosprayed microstructures of tilapia skin collagen

Bi, Chunhuan; Li, Xiaohui; Xin, Qi; Han, Wei; Shi, Cuiping; Guo, Ruihua; Shi, Wenzheng; Qiao, Ruirui; Wang, Xichang; Zhong, Jian

doi:10.1016/j.jbiosc.2019.02.004

Cited by 71 publications

(37 citation statements)

References 62 publications

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“…These gaps may be due to the species specificity of this molecule or differences in sample preparation methods. Bi et al () reported that different extraction methods, including hot water, acetic acid, and sodium hydroxide, have distinct effects on the microstructures of electrospun/electrosprayed collagen tilapia skin using an optical microscope. They attributed this to the difference in molecular weight and protein secondary structure.…”

Section: Discussionmentioning

confidence: 99%

Biochemical study of type I collagen purified from skin of warm sea teleost Mahi mahi ( Coryphaena hippurus ), with a focus on thermal and physical stability

Akita

Kono²,

Lloyd

et al. 2019

J Food Biochem

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Acid‐ and pepsin‐soluble collagen were purified from the skin of mahi mahi (mmASC and mmPSC). The Pro+Hyp content of the latter (185/1,000 residues) was highest among all marine teleost fishes. Fourier transform infrared spectroscopy and Circular Dichroism (CD) analysis showed the typical structure of type I collagen. The ratio of positive over negative peak intensity calculated from the CD spectrum was approximately 1.19 in mmPSC, which is remarkably high, and indicates the stability of the triple helix. The denaturation temperatures (Td) of mmASC and mmPSC were the highest (29.5 and 28.8°C, respectively) among the marine teleost fishes previously studied. atomic force microscope and scanning electron microscope images showed that even after pretreatment, the fibrils presented their structure and fiber orientation. These results indicate the robustness of both collagens, which can be attributed to the high value of Pro+Hyp stabilizing the helix structure of the collagen molecule. Practical applicationsWhile Mahi mahi is highly valuable for its meat, other parts such as skin is not fully utilized in seafood industry. On the contrary, it has been empirically shown that the skin of Mahi mahi has high thermal stability, thus, the skin has been used for leather products in some areas located in the tropical and subtropical zones. In this study, we focused on collagen a major component in skin and investigated the structure and the biochemical characteristics of it. Some results showed that collagen from skin has high physical stability. The collagen from skin of Mahi mahi will be a new fishery resource which could be used as a material for collagen products.

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Section: Discussionmentioning

confidence: 99%

Biochemical study of type I collagen purified from skin of warm sea teleost Mahi mahi ( Coryphaena hippurus ), with a focus on thermal and physical stability

Akita

Kono²,

Lloyd

et al. 2019

J Food Biochem

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“…In view of this, research on collagen nanofibers had been focused on the extraction of collagen from alternative sources, that is, fish collagen and the selection of the electrospinning parameters for this collagen source. For example, in a recent study, Bi et al 98 extracted tilapia skin collagen by three different methods: acetic acid method, hot water method, and sodium hydroxide method; then, the three different collagens were electrospuned. The authors found that the method of extraction had a direct effect on the quantity needed for microfibers production, the concentration required to form fibers varied from 5% for acetic acid collagen up to 34% for sodium hydroxide collagen.…”

Section: Collagen Electrospun Materials For Skin Tissue Engineeringmentioning

confidence: 99%

Collagen based electrospun materials for skin wounds treatment

Hernández-Rangel

Martín‐Martínez

2021

J Biomedical Materials Res

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Materials used for wound care have evolved from simple covers to functional wound dressings with bioactive properties. Electrospun nanofibers show great similarity to the natural fibrillar structure of skin extracellular matrix (ECM); therefore, by mimic, the morphology of ECM, nanofibers show high potential for facilitating the healing of skin injuries. Besides morphology, scaffold composition is another important parameter in the production of bioactive wound dressings. Collagen type I is the main structural protein of skin ECM is biocompatible, biodegradable, and its extraction from animal sources is relatively simple. The fabrication of electrospun wound dressings based on collagen and its blends have been studied for skin tissue engineering applications. This review focus on the new advances of collagen electrospun materials for skin wound treatment. It summarizes the recent research on pristine collagen, collagen blends, and collagen surface modifications on nanofibers mats. Finally, the strategies for three‐dimensional nanofibers production will also be discussed.

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“…PSC-IP is PSC by isoelectric precipitation [37]. AAM, HWM, and SHM are collagens extracted by the acetic acid method, hot water method, and sodium hydroxide method, respectively [26].…”

Section: Functional Group Analysis Using Ftirmentioning

confidence: 99%

“…Some studies on collagen from fish skin have been reported. These studies examined the fan-bellied leatherjacket (Monacanthus chinensis) [17], Gibelion catla and Cirrhinus cirrhosus [18], brownstripe red snapper (Lutjanus vitta) [19], bigeye snapper (Priacanthus macracanthus) [20], sole fish [21], parang-parang (Chirocentrus dorab) [22], Atlantic Codfish (Gadus morhua) and Atlantic Salmon (Salmo salar) [23], unicorn leatherjacket (Aluterus monoceros) [24], Nile perch (Lates niloticus) [25], tilapia (Oreochromis niloticus) [26], Northern Snakehead (Channa argus) [27], puffer fish (Lagocephalus inermis) [28], brown backed toadfish (Lagocephalus gloveri) [29], Amur sturgeon (Acipenser schrenckii) [30], bester sturgeon [31], Ocellate puffer fish (Takifugu rubripes) [32], and channel catfish (Ictalurus punctatus) [33].…”

Section: Introductionmentioning

confidence: 99%

Chemical and Antioxidant Characteristics of Skin-Derived Collagen Obtained by Acid-Enzymatic Hydrolysis of Bigeye Tuna (Thunnus obesus)

et al. 2021

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The utilization of bigeye tuna skin as a source of collagen has been increasing the value of these skins. In this study, the quality of the skin was studied first. The skin after 14 h freeze-drying showed a high protein level (65.42% ± 0.06%, db), no histamine and a lack of heavy metals. The collagens were extracted through acid and acid-enzymatic methods. The enzymes used were bromelain, papain, pepsin, and trypsin. The two highest-yield collagens were pepsin-soluble collagen (PSC) and bromelain-soluble collagen (BSC). Both were type I collagen, based on SDS-PAGE and FTIR analysis. They dissolved very well in dimethyl sulfoxide and distilled water. The pH ranges were 4.60–4.70 and 4.30–4.40 for PSC and BSC, respectively. PSC and BSC were free from As, Cd, Co, Cr, Cu, and Pb. They showed antioxidant activities, as determined by the DPPH method and the reducing power method. In conclusion, bigeye tuna skin shows good potential as an alternative source of mammalian collagen. Although further work is still required, PSC and BSC showed the potential to be further used as antioxidant compounds in food applications. Other biological tests of these collagens might also lead to other health applications.

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Effect of extraction methods on the preparation of electrospun/electrosprayed microstructures of tilapia skin collagen

Cited by 71 publications

References 62 publications

Biochemical study of type I collagen purified from skin of warm sea teleost Mahi mahi ( Coryphaena hippurus ), with a focus on thermal and physical stability

Biochemical study of type I collagen purified from skin of warm sea teleost Mahi mahi ( Coryphaena hippurus ), with a focus on thermal and physical stability

Collagen based electrospun materials for skin wounds treatment

Chemical and Antioxidant Characteristics of Skin-Derived Collagen Obtained by Acid-Enzymatic Hydrolysis of Bigeye Tuna (Thunnus obesus)

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