Effect of Extraction Conditions on the Phenolic Content and DPPH Radical Scavenging Activity of Hypericum perforatum L.

Abstract

In this study, various extracts of Hypericum perforatum L. were investigated in terms of their extraction yields. For this purpose, five solvent systems including 70%EtOH-30% H2O (A), 50%EtOH-50% H2O (B), 30%EtOH-70% H2O (C), 100% EtOH (D), and 100% H2O (E) were used. Ultrasonic-assisted extraction was used for extraction and extraction processes were performed at different temperatures (30°-70oC) and extraction times (30-70 min.). The optimum extraction conditions for H. perforatum was determined based on extraction yield, total phenolic contents, and DPPH radical scavenging activities of the crude extracts, as well. The best ultrasound extraction conditions are achieved in the presence of 70%EtOH-30% H2O solvent system, for 40 min, at 30°C for H. perforatum.

Evaluation of Thymus pseudopulegioides plant extracts for total phenolic contents, antioxidant and antimicrobial properties

“…In contrast, Gedikoglu et al (2019) stated that the total phenolic content of T. vulgaris and T. spicata ranged between 13.13 and 15.13 mg GAE/g for dry weight. The difference in the results of studies in the literature might be probably due to the extraction conditions like solvent type, solvent concentration, extraction temperature and time, and plan parts (Sekeroglu, Urlu, Kulak, Gezici, & Dang, 2017;Seyrekoğlu & Temiz, 2020).…”