Effect of extracellularly generated singlet oxygen on Gram-positive and Gram-negative bacteria

Valduga, Giuliana; Bertoloni, Giulio; Reddi, Elena; Jori, Giulio

doi:10.1016/1011-1344(93)80168-9

Cited by 80 publications

(48 citation statements)

References 18 publications

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“…It is generally accepted that, upon light activation, rose bengal produces singlet oxygen ( 1 O 2 ) in the vicinity of target cells that subsequently die from oxidative stress [30][31][32]. Because singlet oxygen is effective in the nanometer range (20 nm), photosensitizers that tightly bind or penetrate bacterial cells may elicit more antibacterial activity than those producing oxygen derivates nearby cells [14,16,33].…”

Section: Discussionmentioning

confidence: 99%

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

Manoil

Filieri

Schrenzel

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry Antibacterial photodynamic therapy (aPDT) using rose bengal (RB) and blue-light kills bacteria through the production of reactive oxygen derivates. However, the interaction mechanism of RB with bacterial cells remains unclear. This study investigated the uptake efficiency and the antibacterial activity of blue light-activated RB against Enterococcus faecalis and Fusobacterium nucleatum. Spectrophotometry and epifluorescence microscopy were used to evaluate binding of RB to bacteria. The antibacterial activity of RB after various irradiation times was assessed by flow cytometry in combination with cell sorting. Uptake of RB increased in a concentration dependent manner in both strains although E. faecalis displayed higher uptake values. RB appeared to bind specific sites located at the cellular poles of E. faecalis and at regular intervals along F. nucleatum. Blue-light irradiation of samples incubated with RB significantly reduced bacterial viability. After incubation with 10 μM RB and 240 s irradiation, only 0.01% (± 0.01%) of E. faecalis cells and 0.03% (±0.03%) of F. nucleatum survived after treatment. This study indicated that RB can bind to E. faecalis and F. nucleatum in a sufficient amount to elicit effective aPDT. Epifluorescence microscopy showed a yet-unreported property of RB binding to bacterial membranes. Flow cytometry allowed the detection of bacteria with damaged membranes that were unable to form colonies on agars after cell sorting.

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Section: Discussionmentioning

confidence: 99%

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

Manoil

Filieri

Schrenzel

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…33 Assim como nas bactérias, várias abordagens têm sido aplicadas para superar esta barreira de permeabilidade, como a combinação do fotossensibilizador com nonapéptido polimixina 34 ou EDTA. 35 Espécies de fungos não patogênicos, como Saccharomyces cerevisiae, 36 têm sido utilizadas para avaliar o dano da destruição celular induzido por PDI em células eucarióticas. Há relatos na literatura da inativação de Aspergillus fumigatus através da PDI.…”

Section: Fungosunclassified

"Photoantibiotics" the light in the photoinactivation of microorganisms

Lo¹,

Barreira²,

Gonçalves³

et al. 2015

Revista Virtual De Química

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Photo-anti ioti s -the light in the photoinactivation of microorganismsAbstract: The interest in photodynamic inactivation (PDI) has been motivated by the increasing appearance of microorganisms resistant to antibiotics, due to the excessive or inappropriate use of these drugs. PDI involves the combination of a photosensitizer, light and molecular oxygen in order to cause selective destruction of microorganisms. This technique has been proven to be effective against viruses, bacteria, fungi and parasites. This paper aims to highlight some of the results already obtained from the PDI studies.

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“…An increase of the PDI efficacy has been addressed recently both in bacteria using the polycationic biopolymer chitosan [20]. Another method includes the using of metal chelators (ethylenediaminetetraacetic acid (EDTA)) or polypeptide polymixin B [21]. These agents destabilize the lipopolysaccharides coating by removing the Ca…”

Section: Antimicrobial Efficacy Of Pdt: the Photosensitizersmentioning

confidence: 99%

“…and Mg 2+ ions, thereby increasing permeability of the Gram-negative outer membrane and allowing PSs, that are normally excluded from the cell, to penetrate to a location where the reactive oxygen species (ROS) generated on illumination that can execute fatal damage [21]. At present, there is a consensus that aPDT can be effective to kill all known classes of microorganism, including methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant (MDR) and pandrugresistant (PDR) fungi, protozoa, viruses, etc., whether in vitro or in vivo [13].…”

Section: +mentioning

confidence: 99%

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

Bloise¹,

Minzioni²,

Imbriani³

et al. 2017

Photomedicine - Advances in Clinical Practice

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Recent developments in light-controlled therapies (e.g., photodynamic and photothermal therapies) provide promising strategies to prevent and suppress bacterial infections, which are a leading cause of morbidity and mortality. Antibacterial photodynamic therapy (aPDT) has drawn increasing attention from the scientific society for its potential to kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance. In this chapter, we summarize the mechanism of action of aPDT, the photosensitizers, as well the current developments in terms of treating Gram-positive and Gram-negative bacteria. The chapter also describes the recent progress relating to photomedicine for preventing bacterial infections and biofilm formation. We focus on the laser device used in aPDT and on the light-treatment parameters that may have a strong impact on the results of aPDT experiments. In the last part of this chapter, we survey on the various nanoparticles delivering photoactive molecules, and photoactive-nanoparticles that can potentially enhance the antimicrobial action of aPDT.

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Effect of extracellularly generated singlet oxygen on Gram-positive and Gram-negative bacteria

Cited by 80 publications

References 18 publications

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

"Photoantibiotics" the light in the photoinactivation of microorganisms

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

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Effect of extracellularly generated singlet oxygen on Gram-positive and Gram-negative bacteria

Cited by 80 publications

References 18 publications

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

"Photo­antibiotics" ­ the light in the photoinactivation of microorganisms

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

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Product

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"Photoantibiotics" the light in the photoinactivation of microorganisms