Effect of external calcium and magnesium on thrombin-induced changes in calcium and magnesium of pig platelets

Kinlough-Rathbone, R L; Chahil, A; Mustard, J. F.

doi:10.1152/ajplegacy.1973.224.4.941

Cited by 29 publications

(8 citation statements)

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“…4) that the effect of lithium does not correlate with the calcium and/or magnesium content of platelets from different mammals. The large interspecies differences in intraplatelet calcium and magnesium con tents that we found are in agreement with published data (8,11,17,19,20). Although the total intraplatelet calcium concentration is not influenced by lithi um, an interference with the process of the liberation of calcium into the cyto plasm, the area where calcium plays its role, is still possible.…”

Section: Discussionsupporting

confidence: 92%

Observations on ADP Aggregation of Lithium Chloride Incubated Platelets in a Variety of Mammalian Species

Imandt¹,

Tyhuis²,

Wessels³

et al. 1980

Pathophysiol Haemos Thromb

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In citrated platelet-rich plasma of eight mammalian species lithium produces species-dependent effects on ADP aggregation ranging from stimulation to inhibition. Raising the extracellular calcium concentration enhanced aggregation of both lithium-stimulated and lithium-inhibited platelets, indicating that the extracellular calcium concentration has no direct relation to the effect of lithium on platelet function. Lithium did not induce changes in total intracellular platelet calcium and magnesium contents. Aggregation of platelets with ionophore A23187 showed the same species-specific lithium effects as aggregation induced by ADP. The data suggest that lithium does not exert its effect on platelets through interference with intra- or extracellular calcium concentrations.

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Section: Discussionsupporting

confidence: 92%

Observations on ADP Aggregation of Lithium Chloride Incubated Platelets in a Variety of Mammalian Species

Imandt¹,

Tyhuis²,

Wessels³

et al. 1980

Pathophysiol Haemos Thromb

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“…The Ca2+/Mg2+ ratio of 0.98 is similar to that of cattle platelets (1 : 1.4), but mink platelets contain more CaZ+ and Mgz+ than do cattle platelets [12] (the CaZ+ content of cattle platelets is 5.94 pmoles/lO" platelets). Pig platelets are distinguished by their high Mgz+ content (25.2 pmoles/lO" platelets) and have a Ca2'/MgZ+ ratio of 0.44 [26], while human platelets have a higher Ca2+ than Mgz+ content (22 pmoles and 5.5 pmoles/lO" platelets, respectively) [18] .…”

Section: Discussionmentioning

confidence: 99%

Characterization of platelets from normal mink and mink with the Chediak‐Higashi syndrome

et al. 1979

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Bleeding times of mink with the Chediak-Higashi (CH) syndrome was markedly prolonged. Platelet counts were normal but there was an impaired platelet aggregation response to collagen. The metabolic adenine nucleotide pool of platelets from normal and CH mink was labeled with 14C-adenine and the platelets were gel-filtered. Gel-filtered platelets (GFP) from CH mink contained only 37.9% of the adenosine triphosphate (ATP) and 9.6% of the adenosine diphosphate (ADP) found in normal platelets and the ATP/ADP ratio was similar to the 14C-ATP/14C-ADP ratio. Platelet content of Ca2+, Mg2+, and in particular 5-hydroxytryptamine was decreased. When GFP were incubated with thrombin to induce maximal secretion, only negligible amounts of ATP and ADP were released. The specific activity of the extracellular nucleotides approximated that within the platelet. These findings suggest that the stored nucleotide pool in CH platelets is virtually absent and that the abnormalities in platelet function may be due, in part, to the essential absence of secretable ADP and serotonin. The release of Ca2+ and Mg2+ by CH platelets was 56% and 27.8% of normal, respectively.

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“…In preliminary work, we have found significant differences in platelet dense granule peaks in whole cell spectra from five different species. There are known to be species differences in the ATP/ADP ratios concentrations (Dodds, 1978), serotonin levels (Drummond, 1976), and the magnesium/calcium concentration ratios in dense granules (Kinlough-Rathbone et al, 1973). For example, the difference in dense granule levels of Ca2+ and Mg2+ has been proposed by Ugurbil et al (1979) to explain the difference in 31P NMR results for pig and human platelets.…”

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confidence: 99%

Phosphorus nuclear magnetic resonance of bovine platelets

Carroll¹,

Edelheit²,

Schmidt³

1980

Biochemistry

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31P nuclear magnetic resonance (NMR) spectra of bovine platelets at 28 degrees C display prominent peaks from adenine nucleotides in two different environments, the metabolic and storage pools. Addition of 20 mM 2-deoxy-D-glucose and 0.1 mM 2,4-dinitrophenol depletes metabolic ATP, resulting in loss of nucleotide signals at 5, 10, and 19 ppm and leaving peaks at 6.5, 10.5, and 19 ppm that are assigned primarily to the dense granule storage pool. The ATP/ADP concentration ratio of the remaining pools is 1.9 +/- 0.2, with chemical shifts which reflect a more acidic environment for the intragranular nucleotides than for the cytosol. Isolated bovine dense granules give spectra with similar peak positions to those ascribed to the storage pool after metabolic depletion of whole cells. Storage pool nucleotide spectra are highly temperature dependent. Below 20 degrees C, the beta-ATP peak broadens and decreases in area to the point that it is almost undetectable at 0 degrees C, probably reflecting formation of progressively higher molecular weight aggregates at the lower temperatures. The release reaction was followed by 31P NMR after addition of thrombin at 28 degrees C. Spectra of the cell suspension taken at 5-min intervals were compared to spectra of perchloric acid extracts produced in parallel. A new finding of these experiments is that the released nucleotides rapidly undergo a transition to a "NMR silent" form as part of the bovine platelet release reacton. Metabolic breadkdown and external paramagnetic ion interactions did not explain the loss of signal from the storage pool. During release reaction, the nucleotides may be broadened out beyond detection due to immobilization by divalent cation and/or platelet membrane binding.

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Effect of external calcium and magnesium on thrombin-induced changes in calcium and magnesium of pig platelets

Cited by 29 publications

References 0 publications

Observations on ADP Aggregation of Lithium Chloride Incubated Platelets in a Variety of Mammalian Species

Observations on ADP Aggregation of Lithium Chloride Incubated Platelets in a Variety of Mammalian Species

Characterization of platelets from normal mink and mink with the Chediak‐Higashi syndrome

Phosphorus nuclear magnetic resonance of bovine platelets

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