Effect of Expression of UDP-Glucose Pyrophosphorylase Ribozyme and Antisense RNAs on the Enzyme Activity and Carbohydrate Composition of Field-Grown Transgenic Potato Plants

Borovkov, Alex Y.; McClean, Phillip E.; Sowokinos, Joseph R.; Ruud, Susan; Secor, Gary A.

doi:10.1016/s0176-1617(11)81473-2

Cited by 54 publications

(50 citation statements)

References 25 publications

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“…In contrast, Spychalla et al [7] failed to engineer potato plants with an inhibition of enzyme activity greater than 30 % ; however, this level of inhibition was sufficient to decrease sugar content in stored tubers. In other studies, storage of tubers from transgenic potato plants with a 50 % decreased UGPase activity resulted in decreased sucrose levels [9], which is consistent with the important role of UGPase in sugar synthesis. In plants, changes in carbohydrate concentration have frequently been implicated in responses to a variety of stresses, including cold stress.…”

Section: Introductionsupporting

confidence: 81%

Sucrose and light regulation of a cold-inducible UDP-glucose pyrophosphorylase gene via a hexokinase-independent and abscisic acid-insensitive pathway in Arabidopsis

Ciereszko

Johansson

Kleczkowski

2001

Biochemical Journal

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UDP-glucose pyrophosphorylase (UGPase) is a key enzyme producing UDP-glucose, which is involved in an array of metabolic pathways concerned with, among other functions, the synthesis of sucrose and cellulose. An Arabidopsis thaliana UGPase-encoding gene, Ugp, was profoundly up-regulated by feeding sucrose to the excised leaves and by an exposure of plants to low temperature (5°C). The UGPase activity and its protein content also increased under conditions of sucrose feeding and exposure to cold. The sucrose effect on Ugp was apparently specific and was mimicked by exposure of dark-adapted leaves to light. Drought and O2 deficiency had some down-regulating effects on expression of Ugp. The sugar-signalling pathway for Ugp regulation was independent of hexokinase, as was found by using transgenic plants with increased and decreased expression of the corresponding gene. Subjecting mutants deficient in abscisic acid (ABA) to cold stress conditions had no effect on Ugp expression profiles. Okadaic acid was a powerful inhibitor of Ugp expression, whereas it up-regulated the gene encoding sucrose synthase (Sus1), indicating distinct transduction pathways in transmitting the sugar signal for the two genes in A. thaliana. We suggest that Ugp gene expression is mediated via a hexokinase-independent and ABA-insensitive pathway that involves an okadaic acid-responsive protein phosphatase. The data point towards Ugp as a possible regulatory entity that is closely involved in the homoeostatic readjustment of plant responses to environmental signals.

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Section: Introductionsupporting

confidence: 81%

Sucrose and light regulation of a cold-inducible UDP-glucose pyrophosphorylase gene via a hexokinase-independent and abscisic acid-insensitive pathway in Arabidopsis

Ciereszko

Johansson

Kleczkowski

2001

Biochemical Journal

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“…There are a few reports demonstrating ribozyme activity in plant cells, and only two in which the activity is against endogenous targets (Borovkov et al, 1996;McIntyre et al, 1996). Often, in these studies, ribozymes are embedded singly or as multimers in larger antisense sequences.…”

Section: Discussionmentioning

confidence: 99%

“…Included in the set is the 252 multimer linked 3Ј to the ORF of the bar selectable marker gene (RPA85). Fusion of the ribozyme gene to the ORF of a functional gene has been shown to be an effective strategy for expressing ribozymes in mammalian systems (Cameron and Jennings, 1989;Borovkov et al, 1996). This particular construct has several advantages.…”

Section: Discussionmentioning

confidence: 99%

“…Wegener et al (1994) demonstrated that the effective reduction of neomycin phosphotransferase nptII in transient assays (Steinecke et al, 1992) also could be achieved in transgenic tobacco plants. Recently, two endogenous genes encoding UDPglucose pyrophosphorylase in potatoes and a lignin-forming peroxidase in tobacco have been targeted (Borovkov et al, 1996;McIntyre et al, 1996). In both studies, decreased enzymatic activity of the target was observed in transgenic plants containing ribozyme or antisense constructs.…”

Section: Introductionmentioning

confidence: 99%

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Ribozymes Targeted to Stearoyl–ACP Δ9 Desaturase mRNA Produce Heritable Increases of Stearic Acid in Transgenic Maize Leaves

Merlo¹,

Cowen²,

Delate³

et al. 1998

Plant Cell

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Ribozymes are RNAs that can be designed to catalyze the specific cleavage or ligation of target RNAs. We have explored the possibility of using ribozymes in maize to downregulate the expression of the stearoyl-acyl carrier protein ( ⌬ 9) desaturase gene. Based on site accessibility and catalytic activity, several ribozyme constructs were designed and transformed into regenerable maize lines. One of these constructs, a multimer hammerhead ribozyme linked to a selectable marker gene, was shown to increase leaf stearate in two of 13 maize lines. There were concomitant decreases in ⌬ 9 desaturase mRNA and protein. The plants with the altered stearate phenotype were shown to express ribozyme RNA. The ribozyme-mediated trait was heritable, as evidenced by stearate increases in the leaves of the R 1 plants derived from a high-stearate line. The increase in stearate correlated with the presence of the ribozyme gene. A catalytically inactive version of this ribozyme did not produce any significant effect in transgenic maize. This is evidence that ribozymes can be used to modulate the expression of endogenous genes in maize.

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“…These sugars react with amino acids through Maillard browning to form undesirable colours and flavours at frying temperatures. Although the mechanism by which reducing sugars accumulate in cool temperatures is still not completely understood (Barichello et al 1990;Sowokinos 1990;Sowokinos et al 1994;Borovkov et al 1996), the sequence is mobilisation of starch followed by an New Zealand Journal of Crop and Horticultural Science, 1998, Vol. 26 increased synthesis of sucrose and finally the hydrolysis of sucrose to glucose and fructose (Hertog et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Screening the New Zealand potato germplasm collection for resistance to sugar accumulation during low temperature storage

Harvey¹,

Genet

Lammerink

et al. 1998

New Zealand Journal of Crop and Horticultural Science

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The ability of potato (Solanum tuberosum) cultivars to withstand cool storage without accumulating reducing sugars would allow manufacturers of fried potato products to process potatoes year-round without the development of undesirable Maillard browning compounds. More than 600 potato cultivars and selections held by the New Zealand Institute for Crop & Food Research Limited were screened over three seasons to identify material with resistance to cold-induced sweetening. Potato lines were tested 1-2 weeks after harvest using diabetic glucose test tape, and those with glucose levels below 0.1% (1 g/litre) in the potato juices were stored at 4°C for 2 and 4 months. The effect of any changes in sugar levels on chip colour was then reassessed by frying thin slices in oil and measuring the development of Maillard browning in individual crisps by image analysis. Tubers which failed to produce crisps of an acceptable colour were reconditioned at 18-20°C for up to 4 weeks and retested. Lines with potential to resist cold-induced sweetening or with reconditioning potential were retested for an additional two seasons. † Deceased.‡ Retired. H97069

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Effect of Expression of UDP-Glucose Pyrophosphorylase Ribozyme and Antisense RNAs on the Enzyme Activity and Carbohydrate Composition of Field-Grown Transgenic Potato Plants

Cited by 54 publications

References 25 publications

Sucrose and light regulation of a cold-inducible UDP-glucose pyrophosphorylase gene via a hexokinase-independent and abscisic acid-insensitive pathway in Arabidopsis

Sucrose and light regulation of a cold-inducible UDP-glucose pyrophosphorylase gene via a hexokinase-independent and abscisic acid-insensitive pathway in Arabidopsis

Ribozymes Targeted to Stearoyl–ACP Δ9 Desaturase mRNA Produce Heritable Increases of Stearic Acid in Transgenic Maize Leaves

Screening the New Zealand potato germplasm collection for resistance to sugar accumulation during low temperature storage

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