Effect of explant’s position and culture method on shoot proliferation and micro-cuttings for a rare and endangered species, Abeliophyllum distichum Nakai

Abstract

Using either the apical or axillary bud of the endangered species Abeliophyllum distichum Nakai, we tested the effect of bud position and culture method on shoot proliferation and rooting. In shoot proliferation, the axillary bud explant was more effective than the apical bud and the effect was fostered by BA treatment, whereas no differences were observed in shoot elongation by the explant position. Spontaneous rooting was observed in the MS basal medium and resulted in conspicuous differences in the explant position : more than 80% in apical bud explant and 28% in axillary bud explant was achieved, respectively. The positional effects were also observed in BA pre-treatments: generally vertical culture method appeared to be better in shoot proliferation, growth, and rooting than that of the horizontal culture method regardless of the BA pre-treatment duration.The highest shoot multiplication was achieved through the vertical culture method with axillary bud explant, whereas the best shoot elongation and rooting was obtained using the vertical culture method with the apical bud explant. Apical bud explant was superior to axillary bud explant in ex vitro micro-cuttings and revealed a significant difference in shoot growth and root development. The above results suggest that explant position and culture method influence the efficiency of micropropagation for a rare and endangered plant Abeliophyllum distichum.Keywords Abeliophyllum distichum Nakai, Apical or axillary bud explants, Cultural method, Micropropagation

Effects of culture medium composition on the <italic>in vitro</italic> growth of stem cuttings of <italic>Kirengeshoma koreana</italic> Nakai, a rare species in Korea

“…In addition, regeneration from embryogenic callus of old wild ginseng (Panax ginseng) via somatic embryogenesis has been reported (Bae and Choi, 2014). Lee et al (2015) (Kang et al, 2007), and also has been designated as an endangered species B2ab (ii, iii, v) on the International Union for Conservation of Nature (IUCN) Red List (IUCN, 2012). Despite the importance of this species, only a few studies related to its environmental characteristics (Cho et al, 2013), vegetation structure (Kang et al, 2007), and genetic diversity (Chung et al, 2013) have been reported.…”

Volatile Profiles of Five Variants of Abeliophyllum distichum Flowers Using Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry (HS-SPME-GC-MS) Analysis

“…Due to its high scarcity and ecological and geographic value, A. distichum has rarely been studied compared with other plants. However, in 2017, A. distichum was removed from the list of endangered species by the Ministry of the Environment [ 6 ] due to the development of mass breeding techniques [ 7 , 8 ]. Previous phytochemical studies of these plants have focused on just the leaves, and they reported that four phenylethanoid glycosides and two flavonoids are components of these plants [ 9 , 10 , 11 ].…”