Effect of explant density and volume of cultivation medium on in-vitro multiplication of blueberry (Vaccinium corymbosum L.) varieties "Brigitta" and "Legacy"

Abstract

Efecto de la densidad de explantes y el volumen de medio de cultivo sobre la multiplicación in vitro de arándano (Vaccinium corymbosum L.) variedades Brigitta y Legacy Effect of explant density and volume of cultivation medium on invitro multiplication of blueberry (Vaccinium corymbosum L.) varieties "Brigitta" and "Legacy"

In vitro Propagation of Mortiño (Vaccinium floribundum Kunth.)

“…In this study, the combination with the highest concentration of BAP and IAA (10 µM) resulted in better establishment and sprouting of cuttings, obtaining a multiplication rate of 3.35 (Fig. 4 A-C), which was similar to the multiplication rates (3.3 and 2.66) in the Brigitta and Legacy varieties of V. corymbosum L. respectively (Rodríguez and Morales 2015). Different concentrations of BAP have been evaluated in V. floribundum, and indicated that the buds require high concentrations of BAP for their development (Cobo et al 2018).…”

“…The woody plant medium (WPM), is considered to have low salt concentration, and it is suitable for the cultivation of Vaccinium (Debnath 2009). Numerous studies have shown that the WPM medium was the most suitable for the growth of shoots when compared to other seven media tested in the cranberry variety "Bluecrop" (Rodríguez et al 2015, Cüce et al 2013. From this study, most of the workers have used WPM as a basal medium for the micropropagation of blueberries (Wolfe et al 1983).…”

“…Previous reports showed that the density of 25 explants per bottle, with high volumes of WPM medium, gave the best propagation results in V. corymbosum L. (Rodríguez and Morales, 2015). However, the MS induced necrosis of stems and leaves in explants of lingonberry cultivars, which was more visible on media containing zeatin (Ruzić et al 2012).…”

“…Micropropagation of blueberry plants was first reported about 30 years ago, showing that organogenic-based micropropagation methods do not cause genetic variability (Rodríguez and Morales 2015). Micropropagation studies on V. floribundum have been inconclusive, suggesting that additional research is needed (Torres et al 2010).…”