Effect of exogenous phytohormones and sucrose on micropropagation and microtuberisation of Manihot esculenta Crantz var. TMS 96/0023

Fotso,; Elian, Hubert Dieu Beni; Tchouga, Alvine Ornella; Djeuani, Astride C.; Mbouobda, H. D.; Omokolo, Ndoumou Denis

doi:10.5897/ajb2014.13938

Cited by 2 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fogaça et al [18], reported that cassava cultivars (Parazinha and Mantiqueira) produced the most micro-tubers after 45 days of growth when sucrose (80 g/L) in combination with 0.4 µM BAP and 1.6 µM NAA were used in the induction medium. The results obtained in this study is in agreement with the findings of Fotso et al [19] who used 0.4 mg/L NAA or KIN in combination with 10 to 60 g/L sucrose for the culture of a cassava cv. TMS 96/0023 and obtained highest micro-tubers (4 to 5) and fresh weight (403.3 to 408.1 mg) on 30 g/L sucrose.…”

Section: Discussionsupporting

confidence: 93%

“…Cassava micropropagation medium consists of Murashige and Skoog [16] basal medium (4.43 g/L), Myo-inositol (100 mg/L), naphthalene acetic acid (0.01 mg/L), 6-benzylaminopurine (0.05 mg/L), sucrose (30 g/L), and phytagel (7 g/L) at pH 5.70 ± 0.1 (communication from IITA, Nigeria). For micro-tuber induction, the method of Fotso et al [19] was followed with modifications in growth regulators, sucrose and agar concentrations. The medium consists of MS basal medium (4.43 g/L), Myo-inositol (100 mg/L), naphthalene acetic acid (0.01 mg/L), 6-benzylaminopurine (0.04 mg/L), sucrose (30,45,60, 75 and 90 g/L) and phytagel (1.00 g/L) at pH 5.70 ± 0.01.…”

Section: Plant Culture Media Preparationmentioning

confidence: 99%

“…They produced micro-tubers of cassava using 0.4 µM BAP, 1.6 µM NAA, and 80 g/L of sucrose. Fotso et al [19] studied the production of micro-tubers in cassava using 0.1 to 0.6 mg/L NAA, 0.1 to 0.6 mg/L KIN, 0.1 to 0.6 mg/L NAA/KIN ratio and 10 to 60 g/L sucrose, and found that 0.4 mg/L NAA and 0.4 mg/L KIN used separately with 30 g/L sucrose was most effective for micro-tubers production. Also, Elian et al [20] obtained the highest micro-tubers number (4 to 5) and fresh weight (209.4 to 432.0 mg) when 30 or 40 g/L sucrose were used in combination with 0.5 mg/L BAP and 0.05 mg/L NAA for micro-tuberization of cassava.…”

Section: Introductionmentioning

confidence: 99%

“…Protocol for cassava micro-tuber production was reported with wide variation in the response on the induction medium, for instance in cassava cv. TMS 96/0023 [19] and cv. TMS 95/0211 [20].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In-vitro effects of sucrose and ascorbic acid on induction and shelf-life of micro-tuber of cassava (Manihot esculenta Crantz) genotypes

Onejeme¹,

Uchendu²,

Okporie³

2023

World J. Adv. Res. Rev.

View full text Add to dashboard Cite

Background: Cassava production faces threat from postharvest physiological deterioration (PPD). The PPD is usually observed within the first 72 hours of harvest. Therefore, extending the shelf-life of cassava root-tubers by few days could reduce appreciable financial losses. Objective: This study is aimed to investigate effects of sucrose and ascorbic acid on induction and shelf-life of micro-tubers of cassava cultivars using an In-vitro model. Method: Effect of sucrose (30, 45, 60, 75 and 90 g/L) on the micro-tuber induction was determined after 45 days in these cultivars (TME 1333 and TME 2060). The optimal level was later combined with ascorbic acid (0, 25, 50, 75 and 100 mg/L) to investigate its effect on micro-tubers shelf-life during a 10-day storage. Each study was laid out in factorial under CRD with 5 replications. Results: The 30 g/L sucrose (control) produced the utmost number of micro-tubers (4.33), highest length (6.68 cm), and fresh weight (0.1495 g). The cultivar ‘TME 2060’ had longer (5.26 cm) and higher fresh weight (0.1079 g) than ‘TME 1333’ with 5.04 cm and 0.0977 g respectively. Ascorbic acid (100 mg/L) significantly delayed the discolouration of micro-tubers over a 10-day storage. Also, the 100 mg/L ascorbic acid produced the least percentage of deteriorated micro-tubers. Conclusion: Results demonstrate the roles of sucrose and ascorbic acid in micro-tuber formation and antioxidant activity in delaying PPD. Therefore, we propose for In-situ production (via breeding strategy) of vitamin C-fortified cassava varieties in order to control the incidence of PPD and in turn improve farmer`s economy.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Plant Culture Media Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In-vitro effects of sucrose and ascorbic acid on induction and shelf-life of micro-tuber of cassava (Manihot esculenta Crantz) genotypes

Onejeme¹,

Uchendu²,

Okporie³

2023

World J. Adv. Res. Rev.

View full text Add to dashboard Cite

show abstract

Initiation of Cassava Callus Culture and Its Prospect for Starch Production: A Systematic Mapping Study

Mohd Sofri,

Mohamad Puad,

Nik Ahmad Sabri

et al. 2024

JTAS

View full text Add to dashboard Cite

Mass propagation of cassava on several hectares of arable land due to increasing demand for its starch is not feasible due to land availability, pests and disease invasion, and long cultivation period. Plant cell culture technology is a promising solution despite the scarcity of cassava callus culture for starch production applications. Therefore, a systematic mapping study (SMS) was performed to identify the applications of cassava tissue culture and its prospects in starch production and investigate the important parameters for cassava callus culture initiation. The SMS began with formulating research questions (RQs), conducting searches on various databases, collecting and screening related articles, and extracting and mapping the selected articles. A total of 56 of 589 articles in the initial searching phase were chosen to be used as references to answer each RQ. The extracted data indicates that cassava tissue culture was mostly used for micropropagation, while starch production from its tissue culture is still limited. Basal medium and plant growth regulators influence cassava callus culture initiation most. The findings of the SMS offer a better understanding of cassava tissue culture and the prospects of producing cassava starch.

show abstract

Effect of exogenous phytohormones and sucrose on micropropagation and microtuberisation of Manihot esculenta Crantz var. TMS 96/0023

Cited by 2 publications

References 17 publications

In-vitro effects of sucrose and ascorbic acid on induction and shelf-life of micro-tuber of cassava (Manihot esculenta Crantz) genotypes

In-vitro effects of sucrose and ascorbic acid on induction and shelf-life of micro-tuber of cassava (Manihot esculenta Crantz) genotypes

Initiation of Cassava Callus Culture and Its Prospect for Starch Production: A Systematic Mapping Study

Contact Info

Product

Resources

About