Effect of exogenous methanol on glycolate oxidase and photorespiratory intermediates in cotton

Abstract

Application of methanol (MeOH) inhibits photorespiration and enhances growth and yield in C3 plants. However, the underlying cellular and molecular mechanisms are not clear. In this study, we investigated the effects of foliar application of MeOH (30%, v/v) on glycolate oxidase (GO) activity and photorespiratory intermediates in cotton leaves in a field experiment. MeOH treatment significantly inhibited GO activity (by 30% compared with the controls). We also found that endogenous glyoxylate, a photorespiratory intermediate, increased and glycine decreased significantly in MeOH-treated plants. Serine increased significantly in MeOH-treated plants. These results thus demonstrated that exogenous MeOH can modulate GO activity and the production of photorespiratory intermediates, and sheds new lights on our current understanding of how exogenous MeOH inhibits photorespiration and enhances the growth and yield of C3 plants such as cotton.

Methanol and isoprene emissions from the fast growing tropical pioneer species <i>Vismia guianensis</i> (Aubl.) Pers. (Hypericaceae) in the central Amazon forest

“…Methanol production is thought to be initiated during pectin demethylation reactions catalyzed by the enzyme pectin methylesterase (Bai et al, 2014). In this reaction, the hydrolysis of galacturonic acid methyl esters strengthens the cell wall while liberating methanol as a by-product (Hüve et al, 2007;Harley et al, 2007;Bai et al, 2014;Hanson and Roje, 2001).…”

Metabolites Reprogramming and Na+/K+ Transportation Associated With Putrescine-Regulated White Clover Seed Germination and Seedling Tolerance to Salt Toxicity

“…Glycolic acid is an important intermediate metabolite of the photorespiration pathway in higher plants ( Hong et al, 2017 ). The inhibition of glycolic acid metabolism reduces photorespiration rate and photosynthetic rate, resulting in reduced glycine and serine content, growth retardation, and even “respiratory condition death” ( Bai et al, 2014 ; Lu et al, 2014 ). Significantly higher glycolic acid content was only observed in the Put-pretreated white clover seedlings after 7 days of germination under salt stress, indicating that the Put-induced salt tolerance could be related to the glycolic acid pathway ( Figure 7A ).…”

Short-term root flooding and recovery conditions—affected photosynthetic process of common bean plants

“…Glycolate oxidase (GO; EC 1.1.3.1) activity was assayed in leaves (± 0.2 g), as described by Bai et al (2014). The tissues were ground to a powder using a mortar and pestle with liquid nitrogen and homogenized with 5% polyvinylpolypyrrolidone (PVPP) and 50 mM Tris-HCl buffer (pH 7.8) containing 0.01% Triton X-100 and 5 mM dithiothreitol (DTT).…”