Effect of Exercise on the Expression of HSPBP1, PGLYRP1, and HSPA1A Genes in Human Leukocytes

Abstract

The effects of 30-min medium-intensity exercise on the expression of genes encoding heat shock protein 70 (HSPA1A) and its cochaperones HSP-70-binding protein 1 (HSPBP1) and Tag7 (PGLYRP1) in human leukocytes were studied. Transcription activities of HSPA1A and PGLYRP1 genes increased immediately after medium-intensity exercise, while activity of HSPBP1 gene remained unchanged. During recovery after exercise, the expression of HSPA1A gene virtually did not change, while the expression of PGLYRP1 gene continued to increase and after 90 min more than 2-fold surpassed the basal level.

Effects of 6-week specific low-intensity training on selected aerobic capacity parameters and HSPA1A, HSPB1, and LDHb gene expression in high-level rowers

“…Unquestionably, little is known about changes in HSPB1 expression in conditions of physical effort. There were no changes in HSPB1 expression during 30-min moderate intensity exercises among young men as studied by Maltseva et al (2012). In contrast, in our experiment the mean changes in HSPB1 expression in the testing exercise performed before the low-intensity training period were considerable (1.4 fold more than the resting value).…”

“…However, little is known about the impact of exercises of different intensity on expression of these genes in leukocytes. The determination of their expression can be a valuable source of information about metabolic changes and purposeful limitation of applied training loads, especially in high level athletes, among whom the variability of physiological indicators is not very dynamic (Zeibig et al, 2005;Maltseva et al, 2012). The anti-apoptotic effects of proteins that are encoded by HSPA1A and HSPB1 constitute a protective mechanism of the organism during intensive physical exertion, which is accompanied by severe disturbances in homeostasis (Arya et al, 2007).…”

“…Changes in gene expression in these cells suggest a systemic response, although this might not always be the same in each athlete, despite the application of similar training loads (Maltseva et al, 2005). According to Maltseva et al (2012), the expression of HSPB1 compared to HSPA1A during physical exertion has rarely been studied, and no data have been identified for LDHb expression in human leukocytes. LDHb is a marker gene for muscle cells (Yoshioka et al, 2003), and its overexpression hinders oxidative phosphorylation and improves the efficiency of lactate glycolysis.…”

Vitamin C, A and E supplementation decreases the expression of HSPA1A and HSPB1 genes in the leukocytes of young polish figure skaters during a 10-day training camp

“…To our knowledge, there are no similar data available in the literature. The response to physical exercise is not only local but systemic, making analysis of relative gene expression in peripheral blood leukocytes a reasonable indicator of the whole body response to our experimental intervention [28]. Therefore, we hypothesized that antioxidant vitamins would influence the expression in leukocytes of genes which are known to be induced by oxidative stress [2,3].…”

Assessment of fatigue and the overtraining syndrome in female spinning instructors based on a survey and on the expression of selected genes

“…This group of genes includes, among others, genes encoding heat shock proteins (HSP) associated with anti-apoptotic action and interleukins [14]. Despite few studies on the expression of genes under the influence of physical effort [12,13,15], a growing level of IL10 and a falling one of IL6 seem to be crucial for adaptation to physical effort [16]. In turn, chronic inflammation, which is identified with the state of fatigue or overtraining, is characterized by a much higher increase in the concentration of some cytokines as well as the expression of genes encoding them, especially IL6 mRNA [17].…”