Effect of exercise on microglial activation and transcriptome of hippocampus in fluorosis mice

Wang, Jixiang; Yue, Baijuan; Zhang, Xuhua; Guo, Xin; Sun, Zilong; Niu, Ruiyan

doi:10.1016/j.scitotenv.2020.143376

Cited by 37 publications

(23 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the F treatment, the number of ramified microglia in the cortex decreased, and amoeboid microglia increased, accompanied by a decreased average branch length as well as ramification index and an increased solidity parameter. A study verified that the microglia in the hippocampus of mice in the F group were larger, and the branches became thicker and shorter than the control group, which was in line with this study . After 2 mg Se/kg BW/day SeNPs treatment alone, the ramification index was reduced significantly, and the solidity was significantly increased, indicating that the supplementation of 2 mg Se/kg BW/day SeNPs caused the hyperactivation of microglia to a certain extent.…”

Section: Resultssupporting

confidence: 87%

“…A study verified that the microglia in the hippocampus of mice in the F group were larger, and the branches became thicker and shorter than the control group, which was in line with this study. 51 After 2 mg Se/kg BW/ day SeNPs treatment alone, the ramification index was reduced significantly, and the solidity was significantly increased, indicating that the supplementation of 2 mg Se/kg BW/day SeNPs caused the hyperactivation of microglia to a certain extent. Importantly, intragastric administration of 0.5 and 1 mg Se/kg BW/day SeNPs can restore the F-induced morphology change of microglia by mitigating the characteristics of the solidity and ramification index of microglia.…”

Section: Protective Effects Of Senps On the Basal Metabolic Index In ...mentioning

confidence: 95%

See 1 more Smart Citation

Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway

Yang

Hao

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Depression is a mental health problem with typically high levels of distress and dysfunction, and 150 mg/L fluoride (F) can induce depression-like behavior. The development of depression is correlated with neuronal atrophy, insufficient secretion of monoamine neurotransmitters, extreme deviations from the normal microglial activation status, and immune-inflammatory response. Studies found that Se supplementation was related to the improvement of depression. In this study, we applied selenium nanoparticles (SeNPs) for F-induced depression disease mitigation by regulating the histopathology, metabolic index, genes, and protein expression related to the JAK2-STAT3 signaling pathway in vivo. Results showed that F and 2 mg Se/kg BW/day SeNPs lowered the dopamine (DA) content (P < 0.05), altered the microglial morphology, ramification index as well as solidity, and triggered the microglial neuroinflammatory response by increasing the p-STAT3 nuclear translocation (P < 0.01). Furthermore, F reduced the cortical Se content and the number of surviving neurons (P < 0.05), increasing the protein expressions of p-JAK2/JAK2 and p-STAT3/STAT3 of the cortex (P < 0.01), accompanied by the depression-like behavior. Importantly, 1 mg Se/kg BW/day SeNPs alleviated the microglial ramification index as well as solidity changes and decreased the interleukin-1β secretion induced by F by suppressing the p-STAT3 nuclear translocation (P < 0.01). Likewise, 1 mg Se/kg BW/day SeNPs restored the F-disturbed dopamine and noradrenaline secretion, increased the number of cortical surviving neurons, and reduced the vacuolation area, ultimately suppressing the occurrence of depression-like behavior through inhibiting the JAK2-STAT3 pathway activation. In conclusion, 1 mg Se/kg BW/day SeNPs have mitigation effects on the F-induced depression-like behavior. The mechanism of how SeNPs repair neural functions will benefit depression mitigation. This study also indicates that inhibiting the JAK/STAT pathway can be a promising novel treatment for depressive disorders.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Protective Effects Of Senps On the Basal Metabolic Index In ...mentioning

confidence: 95%

Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway

Yang

Hao

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Several studies have reported that treatment with F increases NF-kB in distinct cells, such as neurons [36], renal and cardiac cells [37]. A recent study showed that treadmill running reduced excessive activation of microglia in the hippocampus of mice treated with 24 mgF/kg, together with changes in the pathway of neuroactive ligand-receptor interaction [38]. Our results showed increased expression of proteins related to NF-kB pathway activation in the muscle of susceptible animals treated with F (SII), while the resistant animals (RII) had an increase in proteins that prevent NF-kB signaling pathway activation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice

et al. 2022

View full text Add to dashboard Cite

We compared the parameters related to glucose homeostasis, and liver and muscle proteomes in fluorosis-susceptible (A/J; S) and fluorosis-resistant (129P3/J; R) mice in response to fluoride (F) exposure and exercise. Ninety male mice (45 R-mice and 45 S-mice) were randomized into three groups: (SI; RI) No-F, No-Exercise, (SII; RII) 50 ppm F, No-Exercise, (SIII; RIII) 50 ppm F, Exercise. Overall, mean F concentrations in the plasma and femur were significantly higher in R-mice compared with S-mice. In R-mice, exercise resulted in an increase in F accumulation in the femur. In S-mice, the mean plasma glucose level was significantly higher in Group II compared with Groups I and III. There was an increase in liver proteins involved in energy flux and antioxidant enzymes in non-exercise groups (I, II) of S-mice in comparison with the corresponding groups of R-mice. The results also showed a decrease in muscle protein expression in Group I S-mice compared with their R-mice counterparts. In conclusion, the findings suggest an increased state of oxidative stress in fluorosis-susceptible mice that might be exacerbated by the treatment with F. In addition, fluorosis-susceptible mice have plasma glucose levels higher than fluorosis-resistant mice on exposure to F, and this is not affected by exercise.

show abstract

“…45 The above-mentioned antiinflammatory and antioxidant effects of β-sitosterol are also accomplished through this pathway. In addition, the enrichment of neuroactive ligand-receptor interaction pathway, which is relevant to MDSS for depression, is associated with neuronal differentiation effects such as hippocampal glial cell activation and glioma [46][47][48] and can synergistically inhibit inflammatory responses with PI3K-Akt signaling pathway. 49 Furthermore, the enrichment of inflammation-associated TNF signaling pathway and apoptotic pathway demonstrated that MDSS treatment of CKD combined with depression involves multiple pathways, multiple targets, and signaling networks of inflammation, oxidative stress, and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Preliminary Exploration of Clinical Efficacy and Pharmacological Mechanism of Modified Danggui-Shaoyao San in the Treatment of Depression in Patients with Chronic Kidney Disease

Xia¹,

Ai²,

Pang³

2022

DDDT

View full text Add to dashboard Cite

Background Depression in Chronic Kidney Disease (CKD) seriously affects the prognosis of patients and Modified Danggui-Shaoyao-San (MDSS) is based on the traditional Chinese formula Danggui-Shaoyao-San (DSS) for the treatment of depression, which is further optimized. The aim of this study was to evaluate the clinical efficacy and safety of MDSS for the treatment of depression in CKD, and to explain the molecular mechanism of MDSS for the treatment of depression in CKD through pharmacology and molecular docking. Methods 62 patients were randomly divided into treatment group (treated with MDSS) and control group (treated with placebo) and assessed by Hamilton Depression Scale, and the primary outcome was to evaluate the efficacy of MDSS in improving depressive symptoms and the effect on liver and kidney function, electrolytes. In addition, we identified the core compounds and potential targets of MDSS through the TCMSP database. The GeneCards, OMIM and Disgenet databases were then used to identify molecular targets for CKD and depression. The target protein–protein interaction network was built using STRING database. Core targets were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Molecular docking was used to verify the relationship between core active compounds and proteins. Results Clinical results showed that CKD patients in the MDSS group had significantly improved depressive status with no significant adverse effects. By network pharmacology analysis, we found that the compound-target network mainly contained 47 compounds and 69 corresponding targets. 844 terms were analyzed by GO enrichment, and 254 signaling pathways in KEGG. Molecular docking showed that the top active compounds had high affinity with four targets. Conclusion We preliminarily investigated the efficacy of MDSS in the treatment of depression in CKD and revealed the characteristics of multiple compounds and multiple targets in MDSS.

show abstract

Effect of exercise on microglial activation and transcriptome of hippocampus in fluorosis mice

Cited by 37 publications

References 78 publications

Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway

Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway

Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice

Preliminary Exploration of Clinical Efficacy and Pharmacological Mechanism of Modified Danggui-Shaoyao San in the Treatment of Depression in Patients with Chronic Kidney Disease

Contact Info

Product

Resources

About