Effect of Excess Nitrite on Tests for Indole and the Cholera Red Reaction

We, Beam

doi:10.1128/jb.77.3.328-330.1959

Cited by 7 publications

(1 citation statement)

References 2 publications

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“…The intervals between subcultures were 1 to 3 years. The biochemical characteristics of the test strains were examined by the standard methods (2,15,16). The serovar of the test strain was first determined by the slide agglutination test with serovarspecific antisera.…”

Section: Aichi Prefecturementioning

confidence: 99%

Cholera enterotoxin production in Vibrio cholerae O1 strains isolated from the environment and from humans in Japan

Minami¹,

Hashimoto²,

Abe³

et al. 1991

Appl Environ Microbiol

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Vibrio cholerae 01 strains isolated from various sources in Japan over the years 1977 through 1987 were examined to confirm the presence or absence of the cholera enterotoxin (CT) gene and production of CT and to determine the kappa-phage type. The CT gene was detected in none of 225 isolates from natural waters but was present in all of the 10 isolates from environmental waters implicated in domestic cholera cases, in 64 strains (26.6%) of the 241 isolates from imported seafoods, in 43 strains (95.6%) of the 45 isolates from domestic cholera cases, and in 119 strains (93.7%) of the 127 isolates from imported cholera cases. The results suggest that the CT gene-positive strains of V. cholerae 01 have been imported into Japan through seafoods and/or by travelers. Sporadic cholera cases have resulted in contamination of the surrounding environment, but the CT gene-positive strains may not have persisted in natural waters to serve as a reservoir for epidemic cholera. The commercially available VET-RPLA kit (a latex agglutination kit for immunological detection of CT) detected production of CT in all of the CT gene-positive strains, indicating that there was no silent CT gene in the test strains. There was a strong correlation between the kappa-phage type and the presence or absence of the CT gene, suggesting a significant clonal difference between CT gene-positive and-negative strains. Five CT gene-negative strains isolated from imported cholera cases (travelers with mild diarrhea) induced a considerable amount of fluid accumulation in rabbit and/or suckling mouse intestines, indicating production of an enterotoxic factor(s) other than CT.. It is considered necessary to characterize the fluid accumulation factor(s) and to study dissemination of the CT gene-negative 01 strains producing the enterotoxic factor(s) to assess the public health significance of the CT gene-negative 01 strains distributed in the environment.

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Section: Aichi Prefecturementioning

confidence: 99%