Effect of ethylene on <i>Agrobacterium tumefaciens</i>‐mediated gene transfer to melon

Ezura, Hiroshi; Yuhashi, Ken Ichi; Yasuta, Tsuyoshi; Minamisawa, Kiwamu

doi:10.1046/j.1439-0523.2000.00438.x

Cited by 52 publications

(51 citation statements)

References 23 publications

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“…In in vitro tissue cultures, plant cells often produce ethylene and the amount of ethylene increased while the explants were treated with Agrobacterium in Agrobacterium-mediated transformation, which resulted in reduced efficiency of plant regeneration and gene transfer (Ezura et al 2000;Seong et al 2005). Silver nitrate, as an inhibitor of ethylene production, can be employed to enhance plant regeneration and Agrobacterium-mediated transformation (Seong et al 2005).…”

Section: Artemisia Annua L (Wormwood or Sweet Wormwood)mentioning

confidence: 99%

Effects of silver nitrate on shoot regeneration of Artemisia annua L.

Lei

Hong

Liu

et al. 2014

Plant Biotechnology

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In order to further increase the shoot regeneration frequency of Artemisia annua L., the effects of silver nitrate on this process was investigated in this study. Different concentration of silver nitrate was added to the shoot induction medium, which was MS basic medium containing 1.0 mg l −1 6-benzyladenine (6-BA) and 0.05 mg l −1 α-napthaleneacetic acid (NAA). When 2 mg l −1 silver nitrate was added to the shoot induction medium, the shoot induction frequency and shoot number per explants was significantly higher than that of the control (without silver nitrate). In addition, silver nitrate at all the tested concentrations could significantly reduce callus formation of the explants. Silver nitrate had also positive influence on shoot elongation in the first 20 days. Furthermore, silver nitrate did not affect the sensitivity of A. annua shoots to Kanamycin (KM); therefore, silver nitrate could be used to improve shoot regeneration capacity and frequency in A. annua genetic transformation.

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Section: Artemisia Annua L (Wormwood or Sweet Wormwood)mentioning

confidence: 99%

Effects of silver nitrate on shoot regeneration of Artemisia annua L.

Lei

Hong

Liu

et al. 2014

Plant Biotechnology

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“…Ethylene gas produced by infected explants reduces efficiency of Agrobacterium infection (Nonaka et al 2008b); therefore, in this case, regulation of ethylene is thought to be one of the critical conditions for efficient transformation. Addition of 1 µM of aminoethoxyvinylglycine (AVG), an ethylene inhibitor (Ezura et al 2000), to the IN medium was highly effective for increasing infection efficiency in the proximal region of Black Tosca explants ( Figure 4A, D, G). Kurotanenankin was the most recalcitrant to Agrobacterium infection ( Figure 4C, F), and GFP signal was very weak in infected explants despite the addition of AVG to IN medium (data not shown).…”

Section: Transformation Trials For Other Cucurbita Speciesmentioning

confidence: 99%

Improving the transformation efficiency of Cucurbita species: factors and strategy for practical application

Nanasato

Okuzaki

Tabei

2013

Plant Biotechnology

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Cucurbita species are refractory to transformation. Hence, the only 2 reports published regarding the transformation of Cucurbita species until our successful transformation of C. moschata (cv. Heiankogiku) in 2011. The efficiency was 2.7±1.3% using wounded explants vortexed with whisker suspension. To improve transformation efficiency, transformation experiments were carried out with various ages of cotyledonary explants. The highest efficiency was obtained with 1-day-old (3.2±0.9%) and 2-day-old (3.3±0.8%) explants after germination. Histochemical analysis of GUS activity revealed that wounding allowed Agrobacterium access to the deeper layer of explants. These results suggested that cells having a high ability of shoot organogenesis exist not on the surface but in the deeper layers of explants. We applied vacuum infiltration to wounded explants to enhance Agrobacterium access to the deeper layers, which resulted in improvement of transformation efficiency by 3-fold (9.2±2.9%). To verify the efficacy of our transformation procedure for other Cucurbita species, we attempted to obtain transformants with 3 Cucurbita species: C. maxima (cv. Ebisu), C. pepo (cv. Black Tosca), and C. ficifolia (cv. Kurotanenankin). The average regeneration efficiencies were 54.2±7.2%, 62.5±19.1%, and 72.2±13.4% under our regeneration system. Although the efficiency for production of tansgenic plants was very low (ca. 0.2-0.3%), a transgenic line was obtained from C. maxima and C. pepo, respectively. We discuss recent advances that may help in the development of beneficial applications for the transformation of Cucurbita species.

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“…Galperin (2003) screened melon genotypes for ease of transformation and regeneration and noted variation between 0.4 and 1.5 transgenic shoots per explant. Ezura et al (2000) noted that during Agrobacterium inoculation, explants produced ethylene. By adding an ethylene biosynthesis inhibitor, AVG, to the co-cultivation medium, they reduced ethylene production from the explants, resulting in increased transformation efficiency.…”

Section: Melon (Cucumis Melo)mentioning

confidence: 99%