Effect of Ethylene Action Inhibitors upon Wound-Induced Gene Expression in Tomato Pericarp

Abstract

The contribution of wound-ethylene to wound-induced gene expression was investigated in unripe tomato pericarp using inhibitors of ethylene action. Wounded unripe tomato pericarp was treated with 2,5-norbornadiene or silver thiosulfate to inhibit specifically the induction of ethylene-dependent mRNA species. Poly(A)+ RNAs isolated from these tissues after 12 hours of wounding were translated in vitro in a rabbit reticulocyte lysate system and [35S]methionine-labeled polypeptides were compared to unwounded controls after separation by one and two-dimensional polyacrylamide gel electrophoresis. Results show that mechanical wounding induces a dramatic shift in gene expression (over 50 mRNA species) but expression of less than 15% of these genes is affected by the treatment with ethylene action inhibitors. A selective decrease in mRNAs coding for a 37 kilodalton doublet and 75 kilodalton polypeptides is observed in 2,5-norbomadiene and silver thiosulfate treated wounded pericarp. Levels of hydroxyproline-nch glycoprotein mRNAs induced in wounded tissue were not influenced by inhibitors of ethylene action.

Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis).

“…In our experiments, both ethylene and ABA mimicked the wound-induced accumulation of hevein mRNAs in different rubber tree tissues. These data support the hypothesis that several separate signal transduction pathways can lead to the activation of woundinduced and/or defense related genes in plants (35,37).…”

Involvement of Wound and Climacteric Ethylene in Ripening Avocado Discs

“…ref. 5) (thought to arise from the release ofethylene-mediated inhibition ofethylene production as seen in pulsed preclimacteric fruit [26]), is presumably due to the deinhibition of ACC synthase transcription (13,16). Less likely, stimulation by NBD may to some extent be the result of inhibition of ethylene-mediated diversion of ACC to malonyl-ACC (9).…”

Light and Fungal Elicitor Induce 3-Deoxy-d-arabino-Heptulosonate 7-Phosphate Synthase mRNA in Suspension Cultured Cells of Parsley (Petroselinum crispum L.)

“…98,1992 Light Induction For light induction, several 9-d-old cultures were mixed together and aliquoted as above. Cultures were placed beneath fluorescent lights as described previously (17 (10). Northern blots were performed as described previously (10).…”

“…Cultures were placed beneath fluorescent lights as described previously (17 (10). Northern blots were performed as described previously (10). For each experiment, 10 (Fig.…”