Effect of ethyl acetate extract from husk fiber water of Cocos nucifera in Leishmania braziliensis infected hamsters

Abstract

Abstract:The objective of this study was to evaluate the treatment with ethyl acetate extract (EAE) from husk fiber water of Cocos nucifera L., Arecaceae, in L. braziliensis (Lb) infected hamsters. Twelve male hamsters were randomly allocated in three groups (n=4): G1 received only EAE; G2 was infected with Lb only and G3 received EAE after Lb infection. The infection was carried 28 days prior to the treatment with EAE, which was administrated (0.2 mL, 300 mg.kg -1 ) for 21 consecutive days. Infection was evaluated through skin lesions and infected footpad edema. Haematological evaluation was done on -28 th , 0 and 21 st days. Imprint footpad and lymph node weight were evaluated on 21 st day. Lb infection significantly inhibited the peripheral leukocytes blood. However, neutrophils and lymphocytes values did not have significant alterations. G3 presented eosinophilia in relation to G2. The treatment with EAE did not reduce edema of infected footpad neither weight of drainage lymph node. Infected footpad imprints revealed amastigotes forms and cellular infiltration. Animals from G3 presented skin lesions on 7 th day, shown a reduction of these lesions in day 14. Therefore, the treatment with EAE did not alter the etiological agent elimination in these conditions. However, EAE presents a healing activity in this experimental model.

Cocos nucifera (L.) (Arecaceae): A phytochemical and pharmacological review

“…The lyophilized extract and fractions, as well as ethyl acetate extracts, from the C. nucifera fiber are rich in polyphenols, compounds such as catechins, epicatechins, tannins, and flavonoids ( 7 , 33 – 35 ).…”

“…Ethyl acetate extract (EAE) from husk fiber water was tested against L. braziliensis infected hamsters ( 35 ). Administering EAE (0.2 mL, 300 mg/kg) for 21 consecutive days did not reduce edema of infected footpad nor the weight of lymph node drainage but reduced skin lesions after 14 days.…”

Optimization of Natural Dye Extraction From Coconut Husk

“…Peruvians orally use the aqueous extract of fresh coconut fiber for asthma, diuretics, and gonorrhea (Lima et al, 2015). Presence of polyphenols such as catechins, flavonoids and tannins were reported in high quantity in ethyl acetate extract (Matos, 1997;Freitas et al, 2011;Pal et al, 2011;Renjith et al, 2013). South Indian people utilize fibrous husks for brushing the teeth is a common dental care practice of many rural individuals.…”