Effect of ethanol on human osteosarcoma cell proliferatation, differentiation and mineralization

Vignesh, R.; Djody, S. Sitta; Jayasudha, E.; Gopalakrishnan, V. K.; Ilangovan, R.; Balaganesh, Muthusamy; Veni, S.; Sridhar, M.; Srinivasan, Narasimhan

doi:10.1016/j.tox.2005.11.026

Cited by 19 publications

(16 citation statements)

References 38 publications

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“…The measure of cell proliferation clearly showed that the effect of alcohol consumption was a significant decrease in osteoblast proliferation, in agreement with other authors (Chavassieux et al, 1993;Vignesh et al, 2006;Klein et al, 1996;Klein and Carlos, 1995). The reduction was more evident at the beginning of the experiment, but the difference between EE-OB and the other groups (both ED-OB and SA-OB) remained for 14 days.…”

Section: Discussionsupporting

confidence: 91%

“…Various studies have showed that alcohol alters osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (Gong and Wezeman, 2004;Giuliani et al, 1999). When osteoblast-like cells were exposed, an antiproliferative effect of ethanol was reported in the majority of cases (Vignesh et al, 2006;Klein and Carlos, 1995;Klein et al, 1996;Maran et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Some authors have studied osteoblast behaviour after the in vitro administration of different ethanol concentrations in cultures (Chavassieux et al, 1993;Vignesh et al, 2006;Klein et al, 1996;Klein and Carlos, 1995), and have observed a direct toxic dose-effect on osteoblast proliferation and activity, but others have found that in vitro ethanol treatment had no significant effects on osteoblast number (Maran et al, 2001). Other authors studied the in vivo effects of alcohol consumption on mechanical and reparative properties of bone tissue (Turner et al, 2001b;Brown et al, 2002;Chakkalakal et al, 2002;Hogan et al, 1999;Nyquist et al, 1999Nyquist et al, , 2002Hefferan et al, 2003;Elmali et al, 2002;Chen et al, 2001;Sampson, 1998;Nishiguchi et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Intermittent exposure to ethanol vapor affects osteoblast behaviour more severely than estrogen deficiency does

et al. 2007

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intermittent exposure to ethanol vapor affects osteoblast behaviour more severely than estrogen deficiency does

et al. 2007

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“…In contrast, heavy alcohol consumption, defined as > 1 standard drink/d in women and > 2 standard drinks/d in men (U.S. Department of Agriculture and U.S. Department of Health and Human Services), especially when combined with additional detrimental lifestyle factors such as smoking and poor diet, elicits pathological responses in bone, including reduced osteoblast number and function (Giuliani et al, 1999, Vignesh et al, 2006), disrupted endocrine signaling (Turner et al, 2010, Turner et al, 2012), and decreased BMD (Chappard et al, 1991, de Vernejoul et al, 1983). …”

Section: Introductionmentioning

confidence: 99%

Effects of Chronic Heavy Alcohol Consumption and Endurance Exercise on Cancellous and Cortical Bone Microarchitecture in Adult Male Rats

Johnson

Gaddini

Branscum

et al. 2014

Alcoholism Clin & Exp Res

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Background Bone health is influenced by numerous lifestyle factors, including diet and exercise. Alcohol is a major non-essential constituent of diet and has dose and context-dependent effects on bone. Endurance exercise is associated with increased risk for stress fractures. The purpose of this study was to determine the long-term independent and combined effects of chronic heavy alcohol consumption and endurance exercise (treadmill running) on bone mass and microarchitecture in young adult male Sprague-Dawley rats. Methods Six-month-old male rats were randomized into 4 groups (9–13 rats/group): sedentary+control diet, sedentary+ethanol diet, exercise+control diet, or exercise+ethanol diet. Ethanol-fed rats consumed a liquid diet (ethanol comprised 35% of caloric intake) ad libitum. Control rats were pair-fed the same diet with isocaloric substitution of ethanol with maltose-dextran. Exercise was conducted on a motorized treadmill (15% grade for 30 min) 5 days/week for 16 weeks. Femur and 12th thoracic vertebra were analyzed for bone mineral content (BMC) and density (BMD) using densitometry and cortical and cancellous bone architecture using microcomputed tomography. Results Ethanol consumption resulted in lower femur length, BMC, and BMD, and lower midshaft femur cortical volume, cortical thickness, and polar moment of inertia. In addition, trabecular thickness was lower in vertebra of ethanol-fed rats. Endurance exercise had no independent effect on any endpoints evaluated. A significant interaction between endurance exercise and ethanol was detected for several cancellous endpoints in the distal femur metaphysis. Ethanol-consuming rats that exercised had lower distal femur metaphysis bone volume/tissue volume, trabecular connectivity density, and trabecular thickness compared to exercising rats that consumed control diet. Conclusions The results obtained in this model suggest that chronic heavy alcohol consumption may reduce skeletal integrity by reducing bone size, mass, and density, and by negatively altering cancellous bone microarchitecture and may increase fracture risk associated with endurance exercise at weight bearing skeletal sites.

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“…Taken together, these findings suggest that alcohol has irreversible toxic effects on the osteoblast. This conclusion appears to be supported by in vitro studies reporting that direct exposure to alcohol decreases proliferation of cultured osteoblasts and inhibits their synthesis of bone matrix proteins (Giuliani et al 1999;Vignesh et al 2006). However, the concentrations of alcohol necessary to achieve the detrimental effects in cell culture described above are generally very high, suggesting that mature osteoblasts are quite resistant to direct toxic effects of alcohol (Maran et al 2001).…”

Section: Does Consuming Alcohol Have Irreversible Toxic Effects On Bomentioning

confidence: 72%

Detrimental Effects of Alcohol on Bone Growth

Turner¹,

Doran²,

Iwaniec³

2012

Osteogenesis

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Effect of ethanol on human osteosarcoma cell proliferatation, differentiation and mineralization

Cited by 19 publications

References 38 publications

Intermittent exposure to ethanol vapor affects osteoblast behaviour more severely than estrogen deficiency does

Intermittent exposure to ethanol vapor affects osteoblast behaviour more severely than estrogen deficiency does

Effects of Chronic Heavy Alcohol Consumption and Endurance Exercise on Cancellous and Cortical Bone Microarchitecture in Adult Male Rats

Detrimental Effects of Alcohol on Bone Growth

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