Effect of Ethanol on Brain Metallothionein in Transgenic Mice

Suzuki, Yutaka; Cherian, M. George

doi:10.1111/j.1530-0277.2000.tb04613.x

Cited by 15 publications

(5 citation statements)

References 39 publications

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“…This elevation is seen in all four strains of mice (A/J, BALB/cJ, C57BL/6J, and DBA/2J). It follows reports by Suzuki and Cherian (2000), who noted an increase in brain MTs after ethanol treatment in C57BL/6J mice. This response may be activated through the ARE, seven metal regulatory elements (MREs), and/or other partially characterized regulatory features of the MT-II promoter (Andrews, 2000).…”

Section: Discussionsupporting

confidence: 79%

“…In addition to liver studies, research has shown the level of MT to be elevated in the brain of C57BL/6J mice after oral ethanol administration (Suzuki Cherian, 2000), and suggests that MT-I and MT-II help the brain and central nervous system cope with injury and neurotoxicity (Asanuma et al, 2002;Giralt et al, 2002;Hidalgo et al, 2001). Such results are consistent with the suggestion that the increase of MTs in the brain after ethanol exposure may offer protection against free radicals through their antioxidant properties and/or ability to bind and regulate cellular concentrations of zinc and copper.…”

mentioning

confidence: 99%

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Strain‐Specific Brain Metallothionein II (MT‐II) Gene Expression, Its Ethanol Responsiveness, and Association With Ethanol Preference in Mice

Loney

Uddin

Singh

2003

Alcoholism Clin & Exp Res

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An increase in MT-II mRNA levels after injection of ethanol is attributed to the antioxidant properties of MT-II. The differential mRNA levels of this gene among four strains are not accounted for by the genomic organization, DNA sequence, or methylation status of this gene. Furthermore, the observed correlation between MT-II mRNA levels and ethanol preference raises an interesting hypothesis about the possible role of MT-II in ethanol effects and preference in mice.

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Section: Discussionsupporting

confidence: 79%

mentioning

confidence: 99%

Strain‐Specific Brain Metallothionein II (MT‐II) Gene Expression, Its Ethanol Responsiveness, and Association With Ethanol Preference in Mice

Loney

Uddin

Singh

2003

Alcoholism Clin & Exp Res

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“…Several MT isoforms such as Cg‐MT2 have been described in C. gigas and have been shown to be inducible by metallic stress [56]. Metallothioneins also have diverse physiological functions including protection against oxidants [57]. Murphy et al .…”

Section: Discussionmentioning

confidence: 99%

Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions

et al. 2005

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In the last few decades, marine hypoxia has become one of the major ecological concerns in the world, because of the increase of excessive anthropogenic input of nutrients and organic matter into coastal seawater [1]. Benthic communities are the most sensitive parts of the coastal ecosystem to eutrophication and resulting hypoxia [2]. High production in stratified waters results from nutrient enrichment and can cause hypoxic or anoxic bottom waters because of the subsequent deposition of algal biomass [3]. Marine organisms are directly affected by hypoxia at various levels of organization and behavioural, biochemical and physiological responses to limited availability of oxygen have been well studied in fish and marine invertebrates [4]. Most of the invertebrate species that inhabit the intertidal zone, and especially sedentary ones, have developed mechanisms for surviving twicedaily oxygen deprivation at low tide. Depression of metabolic rate can be considered as one of the most important adaptations for hypoxia endurance [5,6]. Many marine molluscs do indeed show reversible protein phosphorylation to limit the activity of many enzymes and functional proteins during anoxia [5,7]. The same response to hypoxia has already been The molecular response to hypoxia stress in aquatic invertebrates remains relatively unknown. In this study, we investigated the response of the Pacific oyster Crassostrea gigas to hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific genes associated with hypoxia response. A suppression subtractive hybridization method was used to identify specific hypoxia up-and downregulated genes, in gills, mantle and digestive gland, after 7-10 days and 24 days of exposure. This method revealed 616 different sequences corresponding to 12 major physiological functions. The expression of eight potentially regulated genes was analysed by RT-PCR in different tissues at different sampling times over the time course of hypoxia. These genes are implicated in different physiological pathways such as respiration (carbonic anhydrase), carbohydrate metabolism (glycogen phosphorylase), lipid metabolism (delta-9 desaturase), oxidative metabolism and the immune system (glutathione peroxidase), protein regulation (BTF3, transcription factor), nucleic acid regulation (myc homologue), metal sequestration (putative metallothionein) and stress response (heat shock protein 70). Stress proteins (metallothioneins and heat shock proteins) were also quantified. This study contributes to the characterization of many potential genetic markers that could be used in future environmental monitoring, and could lead to explore new mechanisms of stress tolerance in marine mollusc species.Abbreviations GPx, glutathione peroxidase; HIF-1, hypoxia-inducible factor-1; HSP, heat shock protein; MT, metallothionein; SSH, suppression subtractive hybridization.

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“…Similar to environmental stresses, ethanol reduces growth and immunity (Olfati, Mojtahedin, Sadeghi, Akbari, & Martínez‐Pastor, ) and disturbs oxidant and antioxidant balance (Habibian, Ghazi, Moeini, & Abdolmohammadi, ). Ethanol is oxidized into acetaldehyde with simultaneous breakdown of H 2 O 2 and produces acetaldehyde and free radicals (Suzuki & Cherian, ). Ethanol can induce oxidative stress, and it can be used as a model for inducing stress in broiler chicks.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of whey protein sources on performance, liver antioxidants and immune responses of broiler chickens challenged with ethanol

Afkhami

Kermanshahi

Heravi

2020

Animal Physiology Nutrition

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To evaluate the effects of whey protein (WP) sources on performance, liver antioxidants and immune responses of broiler chickens, 300 one‐d‐old male Ross 308 chickens were randomly allocated into six treatments with five replications of 10 birds each. This study was conducted on the basis of a completely randomized design in a 3 × 2 factorial arrangement with dietary supplemental WP sources (no WP; 2% WP and 2% whey protein concentrate (WPC)) and ethanol (zero and 6%) into drinking water. Birds were challenged with ethanol 6% from 10 to 24 days of age. Growth performance, immune responses, intestinal morphology and antioxidant enzymes were assessed in both challenged and non‐challenged groups. There was a significant interaction between ethanol and WP sources for growth performance, so that weight gain (WG), feed intake (FI) and feed conversion ratio (FCR) were significantly improved in broiler chickens challenged with ethanol and fed with WP (p < .05). Intestinal morphology, humoral responses and heterophil to lymphocyte ratio (H/L) were not affected by WP sources. Cellular immunity responses were significantly lower in control group and challenged birds fed with WP compared with other groups after 48 hr of phytohemagglutinin‐P (PHA‐P) injection. Ethanol challenge decreased liver antioxidant enzyme activities and increased levels of malondialdehyde (MDA) and alanine aminotransferase (ALT) activities. Dietary inclusion of WP and WPC increased levels of liver antioxidant enzymes and decreased MDA and ALT activities. It can be recommended to use WP for improving the growth performance and liver antioxidant enzymes in broiler chickens challenged with ethanol.

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Effect of Ethanol on Brain Metallothionein in Transgenic Mice

Cited by 15 publications

References 39 publications

Strain‐Specific Brain Metallothionein II (MT‐II) Gene Expression, Its Ethanol Responsiveness, and Association With Ethanol Preference in Mice

Strain‐Specific Brain Metallothionein II (MT‐II) Gene Expression, Its Ethanol Responsiveness, and Association With Ethanol Preference in Mice

Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions

Evaluation of whey protein sources on performance, liver antioxidants and immune responses of broiler chickens challenged with ethanol

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