Effect of Ethanol Leaf Extract of Spilanthes filicaulis on Liver Indices and Selected Biochemicals on Carbon Tetrachloride Induced Hepatic Damage in Wistar Rats

Abstract

This study investigated the effect of ethanol leaf extract of Spilanthes filicaulis on liver indices and selected biochemicals on carbon tetrachloride induced hepatic damage in wistar rats. A total of thirty (30) rats comprising of five (5) rats per group (90-130 g), were used for the study. Groups 1-3, served as normal, negative (CCl 4 induced 1 mL/kg, 1:1 i.p.) and positive (CCl 4 induced and administered with silymarin 50 mg/kg) controls respectively. Groups 4-6 were the extract treated groups administered with 250 mg/Kg bw, 500 mg/Kg bw and 750 mg/Kg bw respectively for 14 days. The results of the liver marker enzymes showed a significant decrease (p<0.05) in Alanine Transaminase (ALT), Alkaline Phophatase (ALP) and γ-Glutamyl Transferase (GGT) levels in the positive control and extract treated groups, while aspartate transaminase (AST) levels recorded a significant decrease (p<0.05) in groups administered with 250 mg/kg (10.10 ± 0.17 U/L) and 750 mg/Kg bw (11.35 ± 2.01 U/L) when compared with the negative control group. Liver indices, showed no significant difference (p>0.05) in all treated groups when compared to the negative control group. Catalase (CAT) and Superoxide Dismutase (SOD) levels were significantly increased (p<0.05), while Nitric Oxide (NO) and Lipid Peroxidation (LPO) levels significantly decreased (p<0.05) in all groups administered with the extract when compared with the negative control group. Gluthahione levels, lipid profile, renal indices, serum electrolyte and hematological indices showed no significant difference (p>0.05) in all treated groups when compared to the negative control. The study showed that the ethanol leaf extract of Spilanthes filicaulis has potential of restoring hepatic liver damage.

Evaluation of in vitro antioxidants activities, hepatoprotective and haematological effects of ethanol extract of Anthocleista vogelii stem bark (AVSB) on carbon tetrachloride (CCl4) induced rats

“…The marked increases in the activities of serum hepatic marker enzymes including alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) indicated hepatotoxic effects of carbon tetrachloride (CCl4) in the rats induced without any treatment and agrees with earlier findings [32,33]. The serum ALT, AST and ALP activities are usually insignificant due to their very low concentrations in the extrahepatic environment under normal health conditions and their increased activities occur mainly when there are hepatic injuries.…”

“…However, the major hepatoprotective bioactive principle in AVSB needed to be isolated to enable its full utilization in the management of hepatic disorders and diseases with minimal adverse effects. The elevated hepatic enzymes activities in the CCl4 induced rats treated with a lower dose of AVSB suggest that the extract possesses insignificant hepatoprotective activity at a lower dose and requires administration of higher doses to achieve better hepatoprotective effects and are consistent with the earlier findings [32].…”