Effect of estrogen treatment of calcium uptake by the rat uterine smooth muscle

Increase by oestrogen of calcium entry and calcium channel density in uterine smooth muscle

“…Data from our previous study also showed that one hour after oestrogen treatment the increase in calcium uptake was not significant, but it was significant after 24 h and tended to increase thereafter with longer duration of treatment (Batra & Sjogren, 1983). This, together with the data presented here suggests that oestrogen induced the formation of new calcium-channels in myometrial membrane through a genomic mechanism.…”

“…Since calcium plays a key role in the excitation, and excitation-contraction-coupling processes, it would be logical to assume that calcium permeability following oestrogen treatment is altered. Some preliminary evidence for an increase in uterine calcium uptake in vitro after oestrogen treatment in rats has previously been presented (Batra & Sjogren, 1983). One mechanism by which oestrogens could increase calcium entry into the myometrium cell is by inducing the formation ofnew calcium channels in the myometrial membrane.…”

Influence of chronic oestrogen treatment on the density of muscarinic cholinergic receptors and calcium channels in the rabbit uterus

“…Data from recent studies on uterine smooth muscle have shown that oestrogen treatment leads to an increase in transmembrane calcium influx (Batra, 1986), in the density of adrenergic and oxytocin recep¬ tors (Williams & Lefkowitz, 1977;Fuchs, Periyasmi, Alexandrova & Soloff, 1983) and in the number of membrane calcium channels (Batra, 1987). There is also evidence showing that the duration of oestrogen treatment is of considerable importance in the mani¬ festation of both genomic and possibly non-genomic effects of oestrogen (Batra & Sjögren, 1983).…”

Triterpene derivative: A potential signaling pathway for the fern-9(11)-ene-2α,3β-diol on insulin secretion in pancreatic islet

“…The reaction was refluxed under nitrogen for 36 h. Dichloromethane (30 mL) was added and the organic phase was washed with three 25-mL portions of 2 M aq HCl solution, three 25-mL portions of 10% aq NaHCO 3 solution, dried over Na 2 SO 4 , filtered 23 27.9 1.01 s C-4 C-5, C-24 24 16.2 0.91 s C-4 C-5 25 26.1 1.14 s C-10 C-1, C-5, C-9 26 15.3 0.74 s C-14 C-8, C-15 27 15.8 0.81 s C-13 28 13.9 0.76 s C-17 C-16, C-18, C-21 29 23 …”

“…We halted incubation using the technique reported by Batra and Sjogren [28] with modifications [29]. One mL of cold buffer with lanthanum chloride (10 mM) at 2°C was added to the samples to stop calcium fluxes.…”