Effect of Estrogen Deficiency on Skeletal and Alveolar Bone Density in Sheep

Johnson, Roger B.; Gilbert, John E.; Cooper, R.C.; Parsell, Douglas E.; Stewart, Brooke; Dai, X.; Nick, Todd G.; Streckfus, Charles F.; Butler, Ryan; Boring, James

doi:10.1902/jop.2002.73.4.383

Cited by 62 publications

(51 citation statements)

References 51 publications

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“…We collected whole, unstimulated saliva between 12:30 and 1:00 p.m., just prior to the gross anatomy practical examination using the techniques based on those of Navazesh (Navazesh, 1993), which have been commonly used in our laboratory for previous studies (Streckfus et al, 1997;Johnson et al, 2002;McGehee and Johnson, 2004;Johnson, 2005). The students had nothing to eat or drink one hour prior to the saliva collection.…”

Section: Saliva Collectionmentioning

confidence: 99%

Use of saliva for assessment of stress and its effect on the immune system prior to gross anatomy practical examinations

Lester

Brown

Aycock

et al. 2010

Anatomical Sciences Ed

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The objective of this study was to determine the longitudinal effects of a series of stressful gross anatomy tests on the immune system. Thirty-six freshman occupational therapy students completed a written stress evaluation survey, and saliva samples were obtained at baseline and prior to each of three timed-practical gross anatomy tests. Cortisol, secretory IgA (sIgA), and IL-12 concentrations were measured within the salivary samples by enzyme-linked immunosorbent assay. The total scores from the stress surveys were used as markers for environmental stress. Data were compiled for each student at baseline and prior to each examination and were compared by repeated-measures MANOVA and Pearson's correlation test. Following normalization for protein concentration and flow rate, the concentrations of IL-2, IL-6, IL-12, and sIgA progressively increased from baseline to the third test. Cortisol concentrations, following normalization for flow rate, were highest prior to the first test and became significantly reduced prior to second and third test. Prior to second and third test, salivary concentrations of IL-6, IL-2, IL-12, and sIgA were significantly correlated (P < 0.05). In contrast, prior to third test, there was a negative correlation between salivary concentrations of cortisol and IL-12 (P < 0.05). Progressive increases in salivary sIgA, IL-6, IL-2, and IL-12 concentrations from the first to the third test coincident to decreased salivary cortisol suggest that the initial examination stressors precede significant effects on the immune system. These data suggest that there may be latent effects of examination stress on the immune system and that saliva can be used to predict these effects.

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Section: Saliva Collectionmentioning

confidence: 99%

Use of saliva for assessment of stress and its effect on the immune system prior to gross anatomy practical examinations

Lester

Brown

Aycock

et al. 2010

Anatomical Sciences Ed

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“…no, (42) yes (43) Yes (125) Variable: yes, (84,166) no (22) BMD (g/cm 3 ; ±SD) Cancellous-vertebra 0.12-0.14 (82,167) 0.30-0.39 (58) 0.39-0.43 (171) 0.39-0.42 (107) 0.48-0.60 (58,82,168) Cancellous-distal femur 0.43-0.46 (168) 0.44 ± 0.16 (168) - (173) Similarity of bone attributes to humans ; ± SD (when vBMD data not available).…”

Section: Yesmentioning

confidence: 99%

“…(81) Thus far, sheep have proven invaluable in orthopedic research, (82) and their use in the study of alveolar and mandibular bone loss has been effective. (83,84) They are a less expensive animal model than pigs or dogs, and their size is conducive to the insertion of prosthetic implants comparable to those implanted in humans. (85) The effect of osteopenia on the biological response of ovine bone to implants (e.g., osseointegration) and various prosthetic devices can be reliably assessed in studies that allow enough time for a nontransient osteopenic state to develop.…”

Section: Sheepmentioning

confidence: 99%

Review of Nonprimate, Large Animal Models for Osteoporosis Research

Reinwald

Burr

2008

Journal of Bone and Mineral Research

131

105

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Large animal models are required for preclinical prevention and intervention studies related to osteoporosis research. The challenging aspect of this requirement is that no single animal model exactly mimics the progression of this human-specific chronic condition. There are pros and cons associated with the skeletal, hormonal, and metabolic conditions of each species that influence their relevance and applicability to human physiology. Of all larger mammalian species, nonhuman primates (NHPs) are preeminent in terms of replicating important aspects of human physiology. However, NHPs are very expensive, putting them out of reach of the vast majority of researchers. Practical, cost-effective alternatives to NHPs are sought after among ungulate (porcine, caprine, and ovine) and canine species that are the focus of this review. The overriding caveat to using large lower-order species is to take the time in advance to understand and appreciate the limitations and strengths of each animal model. Under these circumstances, experiments can be strategically designed to optimize the potential of an animal to develop the cardinal features of postmenopausal bone loss and/or yield information of relevance to treatment.

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“…The precise relationship between osteoporosis, periodontal mandibular bone, and tooth loss is currently not well understood (7,11,20,29,32,49). Despite the growing evidence that ovariectomy (OVX) could be associated with tooth loss, rather conflicting results have been observed in different animal studies on the effect of OVX on the mandibular bone (22,23,36,45).…”

Section: Introductionmentioning

confidence: 99%

Can Histological and Histomorphometrical Changes be Induced in Rat Mandibular Condyle Following Ovariectomy and Long-Term Extremely Low Frequency Magnetic Field Exposure?

Ülkü

Akdağ

Yavuz

et al. 2012

Biotechnology & Biotechnological Equipment

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The quantity and quality of maxillary and mandibular bone have long been a focus of attention for dental clinicians. The mandibular condyle is a major growth site and plays an important role during mandibular growth. The aim of the present study was to evaluate the effects of long-term extremely low frequency magnetic field (ELF-MF) and hormonal changes produced by bilateral ovariectomy on the histologic and histomorphometric structure of rat mandibular condyle. Forty mature female Sprague-Dawley rats were randomly divided into four different groups (n =10): control (Cnt), ovariectomy (OVX), ELF-MF exposure (ELF-MF), ELF-MF exposure with OVX application (ELF-MF+OVX). All rats were subjected to bilateral ovariectomy except those in Cnt and ELF-MF groups. ELF-MF and ELF-MF+OVX groups were exposed to 1.5 mT ELF-MF during 6 months, 4 h a day. After all applications, left condyle of rats were removed to examine histopathologically. Some histopathologic changes were observed, such as irregular appearance in bone marrow cavities in rat condyles of OVX, ELF-MF and ELF-MF+OVX groups. Some disorders in transition from hypertrophic field to ossification layer and irregular appearance in calcification were determined in OVX group. In OVX+ELF-MF and ELF-MF groups

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Effect of Estrogen Deficiency on Skeletal and Alveolar Bone Density in Sheep

Cited by 62 publications

References 51 publications

Use of saliva for assessment of stress and its effect on the immune system prior to gross anatomy practical examinations

Use of saliva for assessment of stress and its effect on the immune system prior to gross anatomy practical examinations

Review of Nonprimate, Large Animal Models for Osteoporosis Research

Can Histological and Histomorphometrical Changes be Induced in Rat Mandibular Condyle Following Ovariectomy and Long-Term Extremely Low Frequency Magnetic Field Exposure?

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