Effect of estradiol on high glucose‑induced osteoblast injury

Li, Guangrun; Jiang, Xiaofeng; Liu, Liping; Liu, Xiaoyang; Liu, Hongtao; Zhang, Zuofu

doi:10.3892/mmr.2019.10552

Cited by 5 publications

(3 citation statements)

References 42 publications

(40 reference statements)

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“…According to Nie et al, FT3/FT4 levels are positively correlated with serum osteocalcin levels [ 50 ], suggesting the underlying effect of FT3/FT4 on bone remodeling [ 51 ]. And relatively higher serum osteocalcin levels have been clinically proved in patients with hyperthyroidism and high turnover osteoporosis [ 52 ]. Similarly, the results of this work indicated that FT3/FT4 showed a significant negative association with lumbar spine BMD in males.…”

Section: Discussionmentioning

confidence: 99%

Association between Sensitivity to Thyroid Hormone Indices and Bone Mineral Density in US Males

Chen

Huang

Zhou

et al. 2022

International Journal of Endocrinology

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Objectives. Thyroid hormone is acknowledged as a pivotal factor in skeletal development and adult bone maintenance. However, available data about the relationship between sensitivity to thyroid hormone and bone mineral density (BMD) remain limited and conflicting. The purpose of the study was to explore the complex relationship between sensitivity to thyroid hormone indices and BMD using cross-sectional analysis. Methods. An overall sample of 3,107 males from the National Health and Nutrition Examination Survey (NHANES) was studied in the study. The thyroid hormone sensitivity indices included free triiodothyronine/tree thyroxine (FT3/FT4), thyroid-stimulating hormone index (TSHI), thyrotroph thyroxine resistance index (TT4RI), and thyroid feedback quantile-based index (TFQI). Given the complex study design and sample weights, the correlation between sensitivity to thyroid hormone indices and BMD was evaluated through multivariate linear regression models, and extra subgroup analyses were performed to examine the robustness of the results. Results. Among the 3,107 participants, we demonstrated that FT3/FT4 was negatively correlated with lumbar BMD (β = −0.0.35, 95% CI: −0.084–0.013, P < 0.05 ). In the terms of central sensitivity to thyroid hormone, TFQI showed a significant negative relationship with the BMD of the lumbar (β = −0.018, 95% CI: −0.033 to −0.003, P < 0.05 ), total femur (β = −0.020, 95% CI: −0.035 to −0.006, P < 0.01 ), and femur neck (β = −0.018, 95% CI: −0.031 to −0.005, P < 0.01 ). In the subgroup analyses stratified by body mass index (BMI), the significant negative correlation between TFQI and lumbar BMD remained in the male participants with BMI between 18.5 and 24.9 kg/m2. Conclusions. Decreased indices of sensitivity to thyroid hormones are strongly associated with increased lumbar BMD, suggesting that the dysfunction of peripheral and central response to thyroid hormone might contribute to bone loss. In addition, FT3/FT4 and TFQI were considered to be the preferable indicators to guide the prevention and clinical treatment of osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Association between Sensitivity to Thyroid Hormone Indices and Bone Mineral Density in US Males

Chen

Huang

Zhou

et al. 2022

International Journal of Endocrinology

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“…High glucose (HG) induced osteoblast was used to set up a model of diabetes-induced OP in vitro. Previous studies have shown that HG greatly decreased the viability of these cells [9]. Under conditions of high glucose, high-level blood glucose and advanced glycation end-products leads to excessive reactive oxygen species, and causes oxidative stress injury of osteoblasts [10].…”

Section: Introductionmentioning

confidence: 96%

miRNA-seq analysis of high glucose induced osteoblasts provides insight into the mechanism underlying diabetic osteoporosis

Zhang,

Li,

Lou

et al. 2023

Preprint

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Diabetic osteoporosis (DOP) is a chronic complication resulting from diabetes mellitus. The objective of this study is to investigate the underlying pathological mechanisms responsible for bone loss in DOP. Specifically, we aimed to screen the miRNA biomarkers of DOP and investigated whether miRNAs regulate the osteoblasts and thus contribute to DOP. To achieve this, an animal model of DOP was induced through the administration of streptozotocin and subsequent treatment with a high fat diet. Bone microarchitecture and histopathology analysis were carried out. Rat calvarial osteoblasts (ROBs) were induced by high glucose (HG). MiRNA-sequencing was employed to compare the miRNA profiles of HG-induced ROBs and control ROBs. The proliferation and mineralization abilities of ROBs were assessed using MTT assay, alkaline phosphatase, and alizarin red staining. The expression levels of osteoglycin (OGN), Runx2, and ALP were determined through qRT-PCR and Western blot analysis. The miRNA-sequencing results revealed a significant upregulation of miRNA-702-5p. The luciferase reporter gene was utilized in order to investigate the correlation between miR-702-5p and OGN. In vitro experiments demonstrated impaired proliferation and mineralization abilities induced by high glucose. The expressions of OGN, Runx2, and ALP were found to be inhibited. Interference of miR-702-5p resulted in the downregulation of OGN, Runx2, and ALP, which were subsequently upregulated through OGN overexpression. Furthermore, the downregulation of OGN and Runx2 in the femurs of DOP rat models has been confirmed. Consequently, it is reasonable to propose that the miRNA-702-5p/OGN/Runx2 signaling axis could potentially function as a mechanism underlying DOP. This newly discovered mechanism holds promise as a diagnostic biomarker and therapeutic target not only for DOP but also for other forms of osteoporosis.

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“…In patients with OA high osteocalcin serum levels correlate with disease severity and might be used as a specific marker. 13 Osteoblasts are not only responsible for bone formation but also cause bone resorption, through influence on the proliferation, differentiation and fusion of osteoclast progenitors. 14 In vitro studies show inconsistent results, since estrogen has been reported to inhibit, to stimulate or not to have effect on osteoblast proliferation.…”

Section: Introductionmentioning

confidence: 99%

Follicle stimulating hormone and estradiol alter immune response in osteoarthritic mice in an opposite manner

Belenska-Todorova

Zhivkova

Markova

et al. 2021

Int J Immunopathol Pharmacol

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Although a number of studies have shown that the occurrence and progression of osteoarthritis (OA) is related to endocrine system dysfunction, there is limited evidence about what roles sex hormones play. The aim of the present study was to examine the capacity of 17β-estradiol (ED) and follicle stimulating hormone (FSH) to alter the differentiation of bone marrow (BM) cells in arthritic mice. The experiments were conducted in collagenase-induced osteoarthritis in mice. Cartilage degradation was observed by safranin and toluidine blue staining. Flow cytometry was used to define different BM and synovial cell populations. The influence of FSH and ED on osteoclastogenesis was studied in BM cultures and on the osteoblastogenesis in primary calvarial cultures. The levels of IL-8, TNF-α, FSH, and osteocalcin were estimated by ELISA. FSH increased cartilage degradation and serum osteocalcin levels, while ED abolished it and lowered serum osteocalcin. FSH elevated the percentage of monocytoid CD14+/RANK+ and B cell CD19+/RANK+ cells in contrast to ED which inhibited the accumulation of these osteogenic populations. Also, ED changed the percentage of CD105+/F4/80+ and CD11c+ cells in the synovium. FSH augmented and ED suppressed macrophage colony-stimulating factor (M-CSF) + receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast (OC) formation, and this correlated with a respective increase and decrease of IL-8 secretion. FSH did not influence osteoblast (OB) formation while ED enhanced this process in association with changes of TNF-α, IL-8, and osteocalcin production. ED reduced osteoclast generation in bone. The key outcome of the current study is that both hormones influenced BM cell differentiation, with FSH favoring osteoclast formation and ED favoring osteoblast accumulation.

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Effect of estradiol on high glucose‑induced osteoblast injury

Cited by 5 publications

References 42 publications

Association between Sensitivity to Thyroid Hormone Indices and Bone Mineral Density in US Males

Association between Sensitivity to Thyroid Hormone Indices and Bone Mineral Density in US Males

miRNA-seq analysis of high glucose induced osteoblasts provides insight into the mechanism underlying diabetic osteoporosis

Follicle stimulating hormone and estradiol alter immune response in osteoarthritic mice in an opposite manner

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