Effect of estradiol and selected antiestrogens on pro- and antioxidant pathways in mammalian uterus

Jain, Sudhir; Saxena, Deeksha; Kumar, Pratap; Koide, S. S.; Laloraya, Malini

doi:10.1016/s0010-7824(99)00067-0

Cited by 23 publications

(13 citation statements)

References 34 publications

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“…5). To investigate whether SNURF mRNA levels were regulated by estrogen, immature female mice were treated with daily injections of 125 g DES, 50 g of the pure antiestrogen ICI 182,780, 125 g DES and 50 g of ICI 182,780 in combination, or vehicle for 3 d (19,21). SNURF mRNA accumulation was increased to high levels in the oocytes of DES-treated ovaries; this effect was blocked by the antiestrogen ICI 182,780, suggesting that SNURF gene expression is controlled by ER-dependent signaling in the murine oocytes (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In the postovulatory group, ovulation was confirmed by microscopic demonstration of oocytes in the oviduct. In additional experiments, immature FVB female mice (25 d old) were treated daily with sc injections of 125 g diethylstilbestrol (DES; a synthetic estrogen; Sigma-Aldrich), 50 g of the pure antiestrogen ICI 182,780 (Tocris, Bristol, UK), 125 g DES, and 50 g ICI 182,780 in combination or vehicle in 95% mineral oil and 5% ethanol for 3 d (19,21). All the animal studies were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Animal Ethics Committee of the University of Helsinki.…”

Section: Tissue Samplesmentioning

confidence: 99%

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Small Nuclear RING Finger Protein Expression during Gonad Development: Regulation by Gonadotropins and Estrogen in the Postnatal Ovary

et al. 2004

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Small nuclear RING finger protein (SNURF/RNF4) is a steroid receptor coregulator that is down-regulated in testicular germ cell cancer. In this work, we examined SNURF expression during murine fetal gonad development and postnatal ovarian folliculogenesis by in situ hybridization and immunohistochemical staining. SNURF mRNA was detectable in gonads of both sexes from embryonic 10.5 days post conception onward. SNURF protein localized to gonocytes and somatic Leydig and Sertoli cells of fetal testis and in oogonia and supporting cells of fetal ovary. In murine postnatal ovary, SNURF mRNA and protein were expressed throughout folliculogenesis, peaking in the oocytes of preantral follicles. Lower amounts of SNURF mRNA and protein were also present in granulosa cells of secondary, antral, and preovulatory follicles and in luteal glands. Exposure of immature female mice and rats to gonadotropin from pregnant mare serum and human chorionic gonadotropin did not change dramatically SNURF mRNA levels in ovary. SNURF mRNA expression was increased in ovaries of immature mice treated with diethylstilbestrol, an effect that was blocked by the pure antiestrogen ICI 182,780. SNURF protein was constitutively expressed in oocytes of hypophysectomized rats, and its content was augmented by estradiol in granulosa cells. In granulosa cell culture, SNURF mRNA accumulation was transiently increased by treatment with the LH agonists phorbol myristate and forskolin at 4 h after treatment and at 48 h in differentiated cells expressing markers of the preovulatory phenotype. These results suggest a role for SNURF in fetal germ cell development as well as in oocyte and granulosa cell maturation in an estrogen- and gonadotropin-regulated fashion.

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Section: Resultsmentioning

confidence: 99%

Section: Tissue Samplesmentioning

confidence: 99%

Small Nuclear RING Finger Protein Expression during Gonad Development: Regulation by Gonadotropins and Estrogen in the Postnatal Ovary

et al. 2004

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“…We focused our studies on the liver as the center of the body's metabolic activity [13], on the brain and retina in which oxidative stress has been associated with various degenerative diseases and aging [14][15][16], and on the uterus that, besides being the target organ for estrogenic endocrine effect [17], has been studied regarding estrogen-related oxidative stress [18].…”

Section: Introductionmentioning

confidence: 99%

Comparison of estrogen-derived ortho-quinone and para-quinol concerning induction of oxidative stress

Rivera-Portalatin

Vera-Serrano

Prókai-Tátrai

et al. 2007

The Journal of Steroid Biochemistry and Molecular Biology

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Ortho-quinones formed from catechol estrogens are considered prooxidants due to the production of superoxide radical anions through redox cycling via semiquinones. Para-quinols have been identified as novel metabolites of and as the major products of hydroxyl-radical scavenging by estrogens. Cycling of these compounds has also been discovered, because they are converted back to the parent estrogen via reductive aromatization in vitro and in vivo. We hypothesized that, unlike ortho-quinones, para-quinols do not induce oxidative stress due to this cycling. Like the estrogen itself, the 17β-estradiol-derived para-quinol (10β,17β-dihydroxyestra-1,4-diene-3-one) did not induce oxidative stress, as the rate of hydrogen peroxide production during the incubations of the compounds in various tissue homogenates was not significantly different from that of the control experiments performed without the addition of a test compound. We also confirmed that the estrogen metabolite estra-1,5(10)-dien-3,4,17-trione (estrone 3,4-quinone) was a profound prooxidant due to redox cycling, especially in uterine tissue. Therefore, we concluded that para-quinols do not induce oxidative stress.

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“…Moreover, estrogen has prooxidant and antioxidant effects. At estrus, under the influence of estrogen, endometrial epithelial NADPH oxidase produces NADPH (Moulton and Barker, 1971; Hilf et al, 1972; Swanson and Barker, 1983), superoxide anions (Laloraya et al, 1991; Jain et al, 1999, 2000), and hydrogen peroxide (Riley and Behrman, 1991). An estrogen‐stimulated antioxidant system that maintains an optimal amount of these oxidants also regulates sperm capacitation.…”

Section: Discussionmentioning

confidence: 99%

Male Genital Tract Antioxidant Enzymes: Their Source, Function in the Female, and Ability to Preserve Sperm DNA Integrity in the Golden Hamster

Chen

Chow

Cheng

et al. 2003

Journal of Andrology

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Recently, we reported that male accessory sex gland (ASG) secretions protect sperm genomic integrity by demonstrating that DNA damage was more extensive in sperm not exposed to the secretions. The present study was conducted to find out if ASGs secrete the main antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx or GSH-Px), and catalase (CAT) and if the most abundant one, SOD, can protect those sperm that were not exposed to ASG secretions against NADPH-induced oxidative stress. Four experimental groups of male golden hamsters were used: intact animals with proven fertility, animals with all major ASGs removed (TX), animals that were bilaterally vasectomized, and sham-operated controls. SOD, CAT, and GPx activities were measured in secretions from all 5 ASGs and sperm-free uterine flushing from virgin females and those mated with the experimental males. The alkaline comet assay was used to analyze DNA integrity of the TX group sperm after incubation in a medium containing 50 U/mL of SOD along with 0 to 20 mmol/L NADPH. The main antioxidant enzyme in ASGs was SOD from coagulating glands (P <.05) and GPx together with CAT from ampullary glands (P <.05). Uterine flushing of ejaculates that contained ASG secretions had more SOD and CAT activities than those with epididymal secretions alone (P <.05 and P <.001, respectively), whereas activity of GPx was the same (P >.05). Addition of SOD in vitro dose dependently decreased the incidence of single-strand DNA damage in sperm not exposed to ASG secretions incubated in the presence of 0 to 20 mmol/L NADPH (P <.001). These results indicated that, in terms of abundance, SOD was the main antioxidant enzyme secreted by male ASGs, whereas CAT was the second one. The GPx activity came from both epididymis and ASGs. We conclude that ASG secretions play a significant role in protecting sperm against oxidative stress.

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Effect of estradiol and selected antiestrogens on pro- and antioxidant pathways in mammalian uterus

Cited by 23 publications

References 34 publications

Small Nuclear RING Finger Protein Expression during Gonad Development: Regulation by Gonadotropins and Estrogen in the Postnatal Ovary

Small Nuclear RING Finger Protein Expression during Gonad Development: Regulation by Gonadotropins and Estrogen in the Postnatal Ovary

Comparison of estrogen-derived ortho-quinone and para-quinol concerning induction of oxidative stress

Male Genital Tract Antioxidant Enzymes: Their Source, Function in the Female, and Ability to Preserve Sperm DNA Integrity in the Golden Hamster

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