Effect of eslicarbazepine acetate (BIA 2-093) on latrunculin A-induced seizures and extracellular amino acid concentrations in the rat hippocampus

Sierra-Paredes, Germán; Oreiro-García, Maria Teresa; Vázquez-Illanes, Maria Dolores; Sierra‐Marcuño, German

doi:10.1016/j.eplepsyres.2007.08.006

Cited by 18 publications

(18 citation statements)

References 52 publications

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“…However, the need for new experimental models of chronic epilepsy, and for integrating existing models into the process of therapy discovery has been repeatedly pointed out [25,26] . Latrunculin A microperfusion in the rat hippocampus has shown to be an effective model of acute and chronic seizures both for neurochemical studies [17] and for testing novel anticonvulsant drugs [21] . Moreover, latrunculin A microperfusion may be a useful alternative model for studying epileptogenic processes, as seizures are not the consequence of a previous status epilepticus.…”

Section: Discussionmentioning

confidence: 99%

“…Microdialysis methods have been described elsewhere in detail [17,21] . Adult male Swiss mice, weighing 35-40 g were used.…”

Section: Methodsmentioning

confidence: 99%

“…[20] Latrunculin A-induced seizures, as well as neurochemical changes associated to them can be prevented by antiepileptic drugs. [21,22] In the present study we investigated the effect of F-actin depolymerization in the mouse hippocampus on seizure activity and NMDA receptor location. Our results suggest that actin disruption might be not just a consequence but also a possible cause of epileptic seizures.…”

Section: Research Highlightmentioning

confidence: 99%

“…Our results suggest that actin disruption might be not just a consequence but also a possible cause of epileptic seizures. Our new experimental model in mice has shown to be useful to investigate the molecular changes that may lead to chronic seizures, and as a new method for testing antiepileptic drugs [21,22] .…”

Section: Research Highlightmentioning

confidence: 99%

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Partial seizures induced by latrunculin A microperfusion in the mouse hippocampus: Role of extracellular glutamate and NMDA receptors

Alonso-Alonso¹,

Freire-Cobo²,

Vázquez-Illanes³

et al. 2015

Mol Cell Epilepsy

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We have previously reported that in vivo de-polymerization of F-actin filaments induces acute and chronic seizures in mice and rats. On these basis, we have investigated the effect of latrunculin A microdialysis in the mice hippocampus on seizure patterns, F-actin filaments and NMDA receptors. Latrunculin A (8 g/ml) was perfused for three consecutive days into the mice hippocampus using microdialysis probes with continuous EEG and video monitoring. After microdialysis experiments, F-actin depolymerization and synaptic and extrasynaptic NR1 protein levels were investigated. Intrahippocampal latrunculin A microperfusion induced partial seizures during the third day of perfusion, and the animals started showing spontaneous partial seizures one month after treatment. Increased levels of extracellular glutamate via microdialysis probes induced seizures in pre-treated mice. F-actin levels were significantly decreased, while NMDA receptor density increased both in synaptic and non-synaptic locations. These results support the hypothesis that actin disruption might be not just a consequence but also a possible cause of epileptic seizures. Actin depolymerization-induced seizures are related to an increase in synaptic and extrasynaptic NMDA receptors and to changes in the extracellular environment. We propose a new experimental model of epilepsy in mice which has been shown to be useful in the study of the biochemical changes that may lead to chronic seizures, and a new method for testing the efficacy of novel antiepileptic drugs in chronic animals.

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Section: Discussionmentioning

confidence: 99%

“…Microdialysis methods have been described elsewhere in detail [17,21] . Adult male Swiss mice, weighing 35-40 g were used.…”

Section: Methodsmentioning

confidence: 99%

Section: Research Highlightmentioning

confidence: 99%

Section: Research Highlightmentioning

confidence: 99%

See 2 more Smart Citations

Partial seizures induced by latrunculin A microperfusion in the mouse hippocampus: Role of extracellular glutamate and NMDA receptors

Alonso-Alonso¹,

Freire-Cobo²,

Vázquez-Illanes³

et al. 2015

Mol Cell Epilepsy

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show abstract

“…by proconvulsant agents such as metrazole, bicuculline, 4-amino-pyridine, 12 latruncullin A, 32 and picrotoxin. 33 In humans, the clinical trials have focused on partial-onset seizures.…”

Section: Dovepressmentioning

confidence: 99%

Role of eslicarbazepine in the treatment of epilepsy in adult patients with partial-onset seizures

Brown¹,

El‐Mallakh

2010

TCRM

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Eslicarbazepine is a new dibenzazepine antiepileptic agent. It is a high affinity antagonist of the voltage-gated sodium channel. It is closely related to both carbamazepine and oxcarbazepine. Eslicarbazepine has similar affinity to inactivated sodium channels (channels in just activated neurons) as carbamazepine, and greater efficacy in animal models of seizure than oxcarbazepine. In human placebo-controlled trials of a single daily dose of eslicarbazepine added to other anti-epileptic agents, significant seizure reductions occurred with 800 and 1200 mg daily, with nearly half of the patients experiencing a greater than 50% reduction in seizure frequency. Adverse events (AEs) occurred in over 50% of patients receiving therapeutic doses of eslicarbazepine (compared to 31.4%-44.7% of placebo-treated subjects), but were generally mild or moderate. Eight to 19.6% of eslicarbazepine treated patients discontinued due to AEs (compared to 3.9%-8.5% of placebo-treated subjects). In these patients receiving combination anticonvulsant therapy, the most common AEs were dizziness, nausea and vomiting, somnolence, and diplopia. Eslicarbazepine is an effective and reasonably well-tolerated adjunct in patients with suboptimal control of their partial seizures.

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Microdialysis as a Tool to Unravel Neurobiological Mechanisms of Seizures and Antiepileptic Drug Action

Smolders

Clinckers

Michotte

2011

Applications of Microdialysis in Pharmaceutical Science

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Effect of eslicarbazepine acetate (BIA 2-093) on latrunculin A-induced seizures and extracellular amino acid concentrations in the rat hippocampus

Cited by 18 publications

References 52 publications

Partial seizures induced by latrunculin A microperfusion in the mouse hippocampus: Role of extracellular glutamate and NMDA receptors

Partial seizures induced by latrunculin A microperfusion in the mouse hippocampus: Role of extracellular glutamate and NMDA receptors

Role of eslicarbazepine in the treatment of epilepsy in adult patients with partial-onset seizures

Microdialysis as a Tool to Unravel Neurobiological Mechanisms of Seizures and Antiepileptic Drug Action

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