Effect of ergosterol-enriched extracts obtained from Agaricus bisporus on cholesterol absorption using an in vitro digestion model

Gil-Ramírez, Alicia; Ruiz-Rodrı́guez, Alejandro; Marín, Francisco; Reglero, Guillermo; Soler‐Rivas, Cristina

doi:10.1016/j.jff.2014.08.025

Cited by 45 publications

(28 citation statements)

References 31 publications

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“…Cholesterol lowering in serum can be achieved via several mechanisms but mainly by impairing of exogenous cholesterol absorption or inhibiting endogenous cholesterol biosynthesis. The hypocholesterolemic activity of the obtained fractions was evaluated as their ability to inhibit the key enzyme of the cholesterol biosynthetic pathway (HMGCR) since the capacity to impair cholesterol absorption was already evaluated elsewhere for similar extracts containing high ergosterol levels (as ExB) and high β‐glucans and dietary fibers contents (as respectively, ExC and ReC) . The initial shiikate powder showed a remarkable HMGCR inhibitory activity acting as pravastatin used as control (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…Edible mushrooms are natural sources of hypocholesterolemic compounds, such as fungal sterols (ergosterol and derivatives) and polysaccharides (β‐glucans, chitins, etc.) that according to in vitro experiments might inhibit cholesterol absorption and biosynthesis . Eritadenine (2( R ),3( R )‐dihydroxy‐4‐(9‐adenyl) butanoic acid) from Lentinula edodes (shiitake mushrooms) was also able to lower cholesterol levels by acting as an inhibitor of the S‐adenosyl‐L‐homocysteine hydrolase involved in the hepatic phospholipid metabolism .…”

Section: Introductionmentioning

confidence: 99%

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Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Morales

Piris

Ruiz-Rodrı́guez

et al. 2018

Biotechnology Progress

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Three extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies were first extracted with plain water, obtained residue was then submitted to supercritical fluid extraction (SFE) and remaining residue submitted to hot water extraction. Sequential design allowed reutilization of the nonextracted material as raw material for the successive extractions increasing extraction yields and separating interesting compounds. Obtained fractions contained different amounts of ß-glucans, chitins, eritadenine, lenthionine, ergosterol, proteins/peptides and phenolic compounds conferring them different bioactivities. Water soluble fractions showed high antioxidant activities (ABTS and DPPH scavenging capacity and reducing power), they were also able to inhibit one of the main enzymes involved in hypertension (angiotensin-I converting enzyme) and the key enzyme of cholesterol metabolism (3-hydroxy-3-methylglutaryl coenzyme A reductase). The latter inhibitory activity was also noticed in SFE extracts although ergosterol and other lipid-like molecules were isolated. Dietary fibers were separated in the third extraction. Therefore, with this sequential extraction procedure bioactive compounds against CVDs can be selectively separated from a single batch of shiitake powder. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:746-755, 2018.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Morales

Piris

Ruiz-Rodrı́guez

et al. 2018

Biotechnology Progress

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“…In recent years, mycosterols have gained attention from the scientific community as potential functional ingredients for the development of sterol-enriched food products and dietary supplements. 1 Numerous studies have indicated that ergosterol-rich fungi extracts are capable of reducing cholesterol absorption 2,3 and also of inhibiting its biosynthesis in the human body. 4,5 In addition, such extracts promote other health benefits such as anti-cancer, 6 antiproliferative, 7,8 antiinflammatory 9,10 and antimicrobial 11,12 effects.…”

Section: Introductionmentioning

confidence: 99%

A natural food ingredient based on ergosterol: optimization of the extraction fromAgaricus blazei, evaluation of bioactive properties and incorporation in yogurts

et al. 2018

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In recent years, mycosterols have emerged as potential functional ingredients for the development of sterol-enriched food products and dietary supplements. Agaricus blazei is a mushroom rich in bioactive compounds. For commercial purposes, their fruiting bodies must obey rigid morphological criteria. Those not conforming to these criteria are usually discarded, although this does not mean impairment of their content in bioactives. The aim of the present work was to propose the use of commercially discarded A. blazei fruiting bodies for obtaining an extract rich in ergosterol as a fortifier ingredient for yogurts. For extraction, the Soxhlet technology was used and the highest ergosterol yield (around 12%) was achieved in the 5 cycle, yielding 58.53 ± 1.72 µg of ergosterol per 100 g of mushroom (dry weight). The ergosterol rich extract presented notable antioxidant and antimicrobial properties, besides showing no hepatotoxicity. When added to the yogurts it significantly enhanced their antioxidant properties. Furthermore, it did not significantly alter the nutritional or the individual fatty acid profiles of the final dairy products. Thus, A. blazei fruiting bodies that do not conform to the commercial requirements of the market and are normally discarded could be exploited for obtaining a natural high added-value food additive, following the circular bioeconomy concept.

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“…It is the most abundant mycosterol, especially in Agaricus bisporus L. (90% of its sterols fraction) (Barreira et al, 2014;Gil-Ramírez et al, 2014), and has been related with different bioactive properties (Barreira and Ferreira, 2015;Villares et al, 2012). Moreover, ergosterol can be converted by irradiation, into vitamin D for sale as a dietary supplement and food additive (Asinghe and Perera, 2005;Teichmann et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Optimization of microwave-assisted extraction of ergosterol from Agaricus bisporus L. by-products using response surface methodology

Heleno

Prieto

Barros

et al. 2016

Food and Bioproducts Processing

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This work intends to valorise by-products of the industrial processing of mushrooms to obtain ergosterol as a value-added compound. Agaricus bisporus L. is the world's most consumed mushroom and one of the richest sources of ergosterol. Microwave-assisted extraction was used to replace conventional techniques that are time-consuming and need large amounts of solvent. Time (3-20 min), temperature (60-210 ºC) and solid-liquid ratio (1-20 g/L) werefound the relevant variables to analyze the extraction process. To maximize the ergosterol extraction yield, response surface methodology was used to optimize the process. The global optimal extraction conditions were determined and comprise: 19.4±2.9 min, 132.8±12.4 ºC and 1.6±0.5 g/L, yielding 556.1±26.2 mg of ergosterol per 100 g of mushroom by-products.In the MAE optimal conditions, it was possible to obtain ergosterol in a similar value to the one obtained in other works when using the Soxhlet extraction method with a significant decrease in the time of extraction. The results show the potential of using the by-products of an agroindustry, mushrooms processing industry, as productive sources of ergosterol.

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Effect of ergosterol-enriched extracts obtained from Agaricus bisporus on cholesterol absorption using an in vitro digestion model

Cited by 45 publications

References 31 publications

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method

A natural food ingredient based on ergosterol: optimization of the extraction fromAgaricus blazei, evaluation of bioactive properties and incorporation in yogurts

Optimization of microwave-assisted extraction of ergosterol from Agaricus bisporus L. by-products using response surface methodology

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