Effect of eradication of <i>Helicobacter pylori</i> on genetic instabilities in gastric intestinal metaplasia

Tanaka, A.; Watari, Jiro; Tanabe, Hiroki; Maemoto, Atsuo; Fujiya, Mikihiro; Ashida, Toshifumi; Das, Kiron M.; Kohgo, Yutaka

doi:10.1111/j.1365-2036.2006.00045.x

Cited by 12 publications

(20 citation statements)

References 41 publications

(54 reference statements)

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“…The underlying mechanism of the reversal of those molecular events after H. pylori treatment remains elusive. According to the findings of previous reports, it is well known that GIN, a promoter methylation and Das-1 reactivity is associated with chronic inflammatory conditions in gastrointestinal diseases such as BE, 44 chronic gastritis 29,31 and inflammatory bowel disease. 45 The decrease in inflammation in the stomach after the cure of the bacteria may be related to these changes, but it may be difficult to clarify those mechanisms in the current study.…”

Section: Discussionmentioning

confidence: 99%

“…31 Sharma et al have shown that the risk of malignancy is substantially greater in SIM than in conventional intestinal metaplasia in the gastric cardia. 51 Therefore, SIM may have a more malignant potency than gastric intestinal metaplasia from the standpoint of genetic alteration.…”

Section: Discussionmentioning

confidence: 99%

“…Up to now, only a few reports have investigated the genetic alterations comprising MSI and LOH in BE patients, indicating that genetic alterations take place earlier in the development of SIM. 37,38 We previously found that GIN in SIM and gastric intestinal metaplasia may be associated with BE 39 and gastric carcinogenesis, 31,33 respectively. There have so far been very few reports regarding the alteration of E-cadherin in BE and EAC, [40][41][42] and loss of or reduced E-cadherin expression is considered to play a role in the progression of BE to EAC.…”

Section: Discussionmentioning

confidence: 99%

“…As reported previously, 31,33 we examined 5 microsatellite loci on chromosomes for microsatellite instability (MSI), and a loss of heterozygosity (LOH) based on Bethesda panel 34 as follows: 2p (BAT26), 4q (BAT25), 2p (D2S123), 5q (D5S346), and 17p (D17S250). Briefly, polymerase chain reaction (PCR) amplification was carried out in a reaction volume of 10 lL, which contained 100 ng of genomic DNA, 1X PCR buffer (Perkin Elmer Applied Biosystems Division, Foster City, CA), 200 lmol/L of each dNTP, 600 lmol/L of each primer and 1.5 units of AmpliTaq Gold polymerase (Perkin Elmer).…”

Section: Dna Extractionmentioning

confidence: 99%

“…The MSI and LOH status was judged according to previous reports. [31][32][33] No lesions exhibiting MSI at a given locus were evaluated for LOH.…”

Section: Dna Extractionmentioning

confidence: 99%

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Effects of Helicobacter pylori infection on genetic instability, the aberrant CpG island methylation status and the cellular phenotype in Barrett's esophagus in a Japanese population

Moriichi

Watari

Das³

et al. 2008

Intl Journal of Cancer

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Genetic or epigenetic alterations in Barrett's esophagus (BE) with/without Helicobacter pylori (H. pylori) infection remain unclear. We examined the effects of H. pylori infection on genetic instability (GIN), the CpG island methylation status and a biomarker related to BE carcinogenesis. We analyzed 113 Japanese individuals with endoscopically suspected BE. The patients included, Group CLE (n 5 25): no specialized intestinal metaplasia (SIM) in a columnar lined epithelium (control); Group BE (n 5 88): all had SIM. Microsatellite instability and a loss of heterozygosity as GIN, the methylation status at hMLH1, E-cadherin, p16 and APC, and immunoreactivity using a monoclonal antibody (mAb) Das-1, which specifically reacts with BE, were evaluated. Nine additional patients with BE were prospectively followed up for 2 years after successful H. pylori eradication. The frequency of GIN, methylation at E-cadherin and APC, and mAb Das-1 reactivity in Group BE was significantly higher than that in Group CLE (p < 0.0001, p < 0.0001 and p < 0.005, and p < 0.0001, respectively). Furthermore, GIN, E-cadherin methylation and mAb Das-1 reactivity showed a significantly higher incidence in patients with H. pylori infection than in those without H. pylori infection (p < 0.01, p < 0.005, and p < 0.01, respectively). Interestingly, the patients from Group BE were observed to change to a stable state of molecular alterations in 60% for GIN, 42.9% for E-cadherin methylation and 55.6% for APC methylation, or a reduction of mAb Das-1 reactivity was noted in 25% following eradication. H. pylori infection may therefore affect these molecular alterations associated with the pathogenesis of BE, to some degree, in the Japanese population. '

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Dna Extractionmentioning

confidence: 99%

“…The MSI and LOH status was judged according to previous reports. [31][32][33] No lesions exhibiting MSI at a given locus were evaluated for LOH.…”

Section: Dna Extractionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Helicobacter pylori infection on genetic instability, the aberrant CpG island methylation status and the cellular phenotype in Barrett's esophagus in a Japanese population

Moriichi

Watari

Das³

et al. 2008

Intl Journal of Cancer

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Biomarkers predicting development of metachronous gastric cancer after endoscopic resection: an analysis of molecular pathology of Helicobacter pylori eradication

Watari

Moriichi

Tanabe

et al. 2011

Intl Journal of Cancer

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Metachronous gastric cancer (MGC) after endoscopic resection (ER) of gastric cancer still occurs to some degree even after Helicobacter pylori (H. pylori) treatment. We evaluated whether two biomarkers related to carcinogenesis expressed in intestinal metaplasia (IM) become predictors for MGC development after eradication. We performed a hospital-based, case-control study of 75 patients, including 50 mucosal cancer patients who had undergone ER (Group DYS), and 25 age- and sex-matched chronic gastritis patients for whom H. pylori had been successfully eradicated (control). Additionally, Group DYS patients were divided into 2 groups: 25 successfully H. pylori-eradicated (eradicated group) and 25 un-eradicated patients (persistent group). All patients were followed for 1 year. We analyzed microsatellite instability (MSI) and immunoperoxidase assays using a monoclonal antibody for the colonic phenotype (Das-1). Both MSI and Das-1 reactivity in IM were significantly higher in Group DYS than in the control (p<0.05 and p<0.01, respectively). MSI and Das-1 reactivity were strong and independent predictors for gastric cancer (OR=7.09, 95% CI 1.27-39.6, p=0.03 for MSI and OR=4.96, 95% CI 1.64-15.0, p=0.005 for Das-1 reactivity). The incidence of MSI tended to decrease in the eradicated group (p=0.07), but not in the persistent group. The Das-1 immunoreactivity in IM also declined in both the eradicated group and the control. Interestingly, all MGCs after ER were positive for MSI or Das-1 reactivity. MSI or Das-1 reactivity in IM strongly predicts the development of MGC. Patients in whom these biomarkers persist after eradication may therefore have a high risk of developing MGC.

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Chronic Gastritis: Infectious, Non-infectious (Autoimmune, Chemical, Granulomatous), Chronic Superficial and Diffuse Antral Gastritis

Vaiphei

2021

Interpretation of Endoscopic Biopsy - Gastritis, Gastropathies and Beyond

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Effect of eradication of Helicobacter pylori on genetic instabilities in gastric intestinal metaplasia

Abstract: SUMMARY BackgroundThere is little evidence of changes in genetic variations in gastric intestinal metaplasia (GIM) after the eradication of Helicobacter pylori (H. pylori).

Cited by 12 publications

References 41 publications

Effects of Helicobacter pylori infection on genetic instability, the aberrant CpG island methylation status and the cellular phenotype in Barrett's esophagus in a Japanese population

Effects of Helicobacter pylori infection on genetic instability, the aberrant CpG island methylation status and the cellular phenotype in Barrett's esophagus in a Japanese population

Biomarkers predicting development of metachronous gastric cancer after endoscopic resection: an analysis of molecular pathology of Helicobacter pylori eradication

Chronic Gastritis: Infectious, Non-infectious (Autoimmune, Chemical, Granulomatous), Chronic Superficial and Diffuse Antral Gastritis

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