Effect of epinephrine on cyclic adenosine 3′,5′-phosphate and hexose phosphates in intestinal smooth muscle

Bueding, Ernest; Butcher, R.W.; Hawkins, Julia T.; Timms, A. R.; Sutherland, Earl W.

doi:10.1016/0304-4165(66)90061-4

Cited by 63 publications

(12 citation statements)

References 16 publications

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“…In taenia suspended in normal Krebs solution, exposure to adrenaline results in an increase in cyclic 3',5'-AMP (Bueding et al 1966). While, historically, phosphorylase b kinase was the first enzyme which was found to be activated by cyclic 3',5'-AMP, subsequently many other systems and enzymic reactions have been shown to be susceptible to the action of this nucleotide (see Sutherland & Robison, 1966).…”

Section: Discussionmentioning

confidence: 99%

“…Since adrenaline was found to increase the levels of this nucleotide in taenia coli (Bueding et al 1966) it is of interest that, in the presence of the same concentrations of imidazole, the levels of energyrich phosphate compounds were low (Table 12). Moreover, adrenaline failed to increase ATP and CP levels in the presence of imidazole, which also abolished the physiological effect of adrenaline (Fig.…”

Section: Adrenaline and Atpmentioning

confidence: 99%

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The effect of adrenaline on the adenosine otriphosphate and creatine phosphate content of intestinal smooth muscle

Bueding¹,

Bülbring²,

Gercken³

et al. 1967

The Journal of Physiology

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SUMMARY1. The smooth muscle of guinea-pig taenia coli was used to investigate the relation between metabolic and physiological effects of adrenaline. Electrical and mechanical activity was recorded with the sucrose gap technique and, in parallel experiments, the concentration of energy-rich phosphate compounds, adenosinetriphosphate and creatine phosphate, (ATP and CP) in the tissue was determined.2. Adrenaline (in concentrations from 2 x 10-9 g/ml. to 5 x 108 g/ml.) increased the tissue content of energy-rich phosphate compounds. This effect was coincident with the physiological, inhibitory effect on electrical and mechanical activity.3. After anaerobic exposure of the tissue to substrate-free medium, the biochemical and the physiological effects ofadrenaline were both abolished; both recovered after readmission of oxygen and/or substrate.4. In muscles depleted of glycogen in substrate-free medium, either by anoxia or by high temperature, adrenaline produced its stabilizing effect on the cell membrane and the increase in ATP and CP content when ,6-hydroxybutyrate was the substrate, i.e. in the complete absence of carbohydrate from the medium and from the tissue.5. When adrenaline was applied simultaneously with the readmission of substrate, the ATP and OP content of pieces treated with adrenaline was greater than of control pieces, though the tension of both was zero. This indicates that the effect was not secondary to the muscle relaxation but was the result of increased ATP synthesis.6. The physiological and biochemical effects of adrenaline were both abolished by the same concentration of imidazole (0.05 M).E. BUEDING AND OTHERS 7. Low concentrations of ATP (1 x 106-5 x10-6M)-like adrenalineinhibited electrical and mechanical activity of the taenia. This effect was also abolished by imidazole.

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Section: Discussionmentioning

confidence: 99%

Section: Adrenaline and Atpmentioning

confidence: 99%

The effect of adrenaline on the adenosine otriphosphate and creatine phosphate content of intestinal smooth muscle

Bueding¹,

Bülbring²,

Gercken³

et al. 1967

The Journal of Physiology

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“…Therefore, the depressive effect of Ep on the contractile response of colonic tissue to ACh may be attributed to stimu lation of adrenergic beta-receptors. Stimulation of beta-receptors reportedly increases the concentration of intracellular cyclic AMP (4)(5) . Papaverine, which inhibits phos phodiesterase (14--I5), relaxes smooth muscles.…”

Section: Catecholaminesmentioning

confidence: 99%

“…The stimulation of beta-receptor is considered to activate adenylate cyclase and increase the content of intra cellular adenosine 3',5 monophosphate (cyclic AMP) (4)(5). How the drugs which modified intracellular content of cyclic AMP affect the contractile response to ACh, and whether the change of content of intracellular cyclic AMP correlated with relaxation or depression of the contractile responses caused by catecholamines, were also investigated.…”

Section: Koshiba (I) Reported That Catecholaminesmentioning

confidence: 99%

Depressive Effect of Epinephrine Mediated via Adrenergic Beta-Receptor in Isolated Rat Colon and Duodenum

Nakahata

Nakanishi

Suzuki

1977

Japanese Journal of Pharmacology

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“…0022-3751/80/1970-0535 $07.50 © 1980 The Physiological Society INTRODUCTION f3-adrenergic agents suppress the electrical and mechanical activities of various visceral smooth muscles. Since Bueding, Butcher, Hawkins, Timms & Sutherland (1966) suggested that this effect was related to activation of adenyl cyclase, many investigators have shown that the cyclic AMP level in smooth muscle is directly related to smooth muscle tone, i.e. increase in cyclic AMP causes relaxation of visceral smooth muscle (Andersson, Lundholm, Mohme-Lundholm & Nilsson, 1972;Schultz, Schultz & Hardman, 1975;Bar, 1974;Kroeger & Marshall, 1974;Triner, Nahas, Vullienoz, Overweg, Verosky, Habif & Ngai, 1975;Kroeger, Teo, Ho & Wang, 1975;Honeyman, Merrian & Fay, 1978).…”

mentioning

confidence: 99%

Does activation of cyclic AMP dependent phosphorylation induced by beta‐adrenergic agent control the tone of vascular muscle?

Hirata¹,

Kuriyama²

1980

The Journal of Physiology

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SUMMARY1. The relaxing action of the f3-adrenergic agent, isoprenaline, on the porcine coronary artery was investigated in relation to the cyclic AMP level, the endogenous binding of cyclic AMP to the regulatory unit of cyclic AMP dependent protein kinase or the phosphorylation as a result of activation of protein kinase of the muscle homogenate in Krebs solution and excess [K]o solution. These relations were also compared with those of the rat cardiac muscle, in which isoprenaline showed a positive inotropic action.2. Excess [K]0 decreased the cyclic AMP level in proportion to the amplitude of K-induced contracture in the porcine coronary artery. Isoprenaline increased the cyclic AMP level in Krebs solution, while it had no effect in excess [K]o.3. In the porcine coronary artery, the particulate fraction possessed only 5 % of the total cyclic AMP dependent protein kinase, while in the rat cardiac muscle, the particulate fraction was 25 % of the total protein kinase.4. The cyclic AMP dependent protein kinase in the particulate fraction of the porcine coronary artery was already saturated with the endogenous cyclic AMP. However, the binding of cyclic AMP to the protein kinase in the particulate fraction in the cardiac muscle and in the cytosol fraction of both tissues were increased in accordance with the cyclic AMP level. In the coronary artery, the protein kinase in the cytosol fraction was bound to a greater extent with cyclic AMP than was measured in the rat cardiac muscle.5. In the rat cardiac muscle, isoprenaline enhanced the phosphorylation, detected by autoradiography of SDS gel electrophoresis in individual fractions of phosphorylated protein, while little enhancement was observed in the porcine coronary artery. 6. These observations led to the conclusion that in the porcine coronary artery, fl-adrenergic agent increases the levels of cyclic AMP but does not increase the phosphorylation. If the phosphorylation catalyzed by cyclic AMP dependent protein kinase was utilized for Ca mobilization in the cell, the change in the cyclic AMP level would probably not have a causal relation to the muscle tone. This conclusion, however, may not be applicable in the case of the cardiac muscle.

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Effect of epinephrine on cyclic adenosine 3′,5′-phosphate and hexose phosphates in intestinal smooth muscle

Cited by 63 publications

References 16 publications

The effect of adrenaline on the adenosine otriphosphate and creatine phosphate content of intestinal smooth muscle

The effect of adrenaline on the adenosine otriphosphate and creatine phosphate content of intestinal smooth muscle

Depressive Effect of Epinephrine Mediated via Adrenergic Beta-Receptor in Isolated Rat Colon and Duodenum

Does activation of cyclic AMP dependent phosphorylation induced by beta‐adrenergic agent control the tone of vascular muscle?

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