Effect of Epigallocatechin Gallate on the Properties of Gelatin

Gelatin–epigallocatechin gallate nanoparticles with hyaluronic acid decoration as eye drops can treat rabbit dry-eye syndrome effectively via inflammatory relief

“…EGCG cross-linking enhanced the thermal property of gelatin. 41 The endothermal peak of gelatin powder mixed with EGCG powder indicated no chemical reaction or influence for the peak (169.0°C) (data not shown). The peak at 165.6°C in GEH indicated the presence of HA (with a peak of 166.7°C).…”

“…The helix-coil transition formation in GE results in increased decomposition temperature of GE or GEH compared with raw gelatin. 41,55 We observed that hydrogen bonding ameliorated the thermal properties of gelatin, which resulted in the endothermal peak shifting to higher temperatures ( Figure 2C). One of the advantages for NP-mediated drug delivery is controlled release.…”

“…21,24,29,53 Protein-EGCG interactions drive the self-assembly of EGCG with albumin-dextran to form NPs. 54 EGCG has multiple phenolic hydroxyl groups in its structure, 41,55 which drives polyphenol-protein (gelatin) precipitation because of the electrical attraction between EGCG and gelatin-peptide chains, which in turn leads to particle aggregation and the formation of compact spheres at a proper concentration. 41,55 Here, gelatin (type A), which is enriched in positive charges, attracted EGCG, which is enriched in OH groups, to form GE-NPs ( Figure 1A).…”

“…54 EGCG has multiple phenolic hydroxyl groups in its structure, 41,55 which drives polyphenol-protein (gelatin) precipitation because of the electrical attraction between EGCG and gelatin-peptide chains, which in turn leads to particle aggregation and the formation of compact spheres at a proper concentration. 41,55 Here, gelatin (type A), which is enriched in positive charges, attracted EGCG, which is enriched in OH groups, to form GE-NPs ( Figure 1A). Elsewhere, studies of EGCG loaded in gelatin hydrogels documented that gelatin supplies increased stability and encapsulation capacity for EGCG in aqueous solutions via hydrogen bonds.…”

“…Elsewhere, studies of EGCG loaded in gelatin hydrogels documented that gelatin supplies increased stability and encapsulation capacity for EGCG in aqueous solutions via hydrogen bonds. 41,56 EGCG/ gelatin NPs facilitated the stability of EGCG during storage or transport. FTIR revealed a gelatin peak .3,300 cm −1 for the hydrogen-bonded hydroxyl groups, and the amide I band was altered to lower frequencies (1,623-1,629 cm −1 ) in the GE and GEH pattern ( Figure 2B).…”