Effect of epidermal growth factor on the labeling of the various RNA species and of nuclear proteins in primary rat astroglial cell cultures

Avola, Rosanna; Condorelli, D. F.; Turpeenoja, L.; Ingrao, F.; Reale, S.; Ragusa, N.; Stella, A. M. Giuffrida

doi:10.1002/jnr.490200109

Cited by 27 publications

(7 citation statements)

References 34 publications

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“…Primary cultures of astrocytes were prepared from newborn albino rat brains (from 1‐ to 2‐day‐old Wistar strain rats) as previously described (Avola et al, 1988a, 1988b; Bramanti et al, 2007). In particular, cerebral tissues, after dissection and careful removal of the meninges, were mechanically dissociated through 82‐mm‐pore sterile mesh (Nitex).…”

Section: Methodsmentioning

confidence: 99%

“…Astrocyte activity may be modulated by steroid hormones and growth factors. Astrocytes express estrogen receptors (ERs) and glucocorticoid receptors (Avola et al, 1988b; Chou et al, 1991; Jung‐Testas et al, 1992; Sato et al, 2003; Chaban et al, 2004; Pawlak et al, 2005) in both the nucleus and the cell membrane, as well as receptors for growth factors, such as epidermal growth factor (EGF), insulin‐like growth factor–I (IGF‐I), and insulin (INS) receptors (Mazzoni and Kenigsberg, 1994; Jafferali et al, 2000; Aberg et al, 2003; Morita et al, 2005). Steroids regulate the interaction between glia and neurons and glial gene expression and promote the production of astrocytic growth factors, which then may act in both an autocrine and a paracrine fashion (Azcoitia et al, 2001).…”

mentioning

confidence: 99%

“…Glucocorticoids as well as estrogens are involved in neuronal and astroglial cell differentiation and in neurodegeneration/neuroprotection processes in the CNS. Growth factors (GFs) such as basic fibroblast growth factor (bFGF), EGF, IGF‐I, and INS act as mitogenic peptides and participate in neuron–glia cross talk, stimulating cultured nerve cell proliferation and differentiation (Avola et al, 1988a, 1988b; Marletta et al, 2001; Spina et al, 2002). In particular, GFs play a pivotal role in the dynamic flow of signaling molecules between neurons and astroglia (Avola et al, 2000).…”

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confidence: 99%

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Effect of growth factors and steroids on transglutaminase activity and expression in primary astroglial cell cultures

Campisi

Bramanti

Caccamo

et al. 2007

J of Neuroscience Research

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Type-2 transglutaminase (TG-2) is a multifunctional enzyme involved in the regulation of cell differentiation and survival that recently has been shown to play an emerging role in astrocytes, where it is involved in both proliferation and differentiation processes. Growth factors (GFs) such as EGF, basic fibroblast growth factor, insulin-like growth factor-I (IGF-I), and insulin (INS) are trophic and mitogenic peptides that participate in neuron-glia interactions and stimulate neuronal and astroglial proliferation and differentiation. Steroid hormones such as glucocorticoids and estrogens also play a pivotal role in neuronal and astroglial proliferation and differentiation and are key hormones in neurodegenerative and neuroprotective processes. We investigated the effects of the interaction of GFs with dexamethasone (DEX) or 17beta-estradiol (E(2)) on TG-2 activity and their expression in cultured astrocytes. We observed a significant increase in TG-2 activity and expression in astroglial cells treated for 24 hr with IGF-I, EGF, or INS. Priming of the cells with DEX or E(2), for 48 hr also led to an increase in TG-2 levels. When growth factors were present in the last 24 hr of the steroid treatment, a reduction in TG-2 expression and activity and a different subcellular TG-2 distribution were found. Our data indicate that steroid hormone-GF interaction may play an important role in astroglial function. The effect on TG-2 could be part of the regulation of intracellular pathways associated with the astrocyte response observed in physiological conditions and, possibly, also in neuropathological diseases.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Effect of growth factors and steroids on transglutaminase activity and expression in primary astroglial cell cultures

Campisi

Bramanti

Caccamo

et al. 2007

J of Neuroscience Research

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“…Primary cultures of astrocytes were prepared from newborn albino rat brains (1-2-day-old Wistar strain) as previously described [7,14,15]. In particular, cerebral tissues, after dissection and careful removal of the meninges, were mechanically dissociated through sterile meshes of 82 mm pore size (Nitex).…”

Section: Astroglial Cell Culturesmentioning

confidence: 99%

Growth Factors and Steroid Mediated Regulation of Cytoskeletal Protein Expression in Serum-Deprived Primary Astrocyte Cultures

et al. 2008

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In this research we aimed to investigate the interactions between growth factors (GFs) and dexamethasone (DEX) on cytoskeletal proteins GFAP and vimentin (VIM) expression under different experimental conditions. Condition I: 24 h pretreatment with bFGF, subsequent 72 h switching in serum-free medium (SFM) and final addition of GFs, alone or by two in the last 24 h, after a prolonged (60 h) DEX treatment. Condition II: 36 h pretreatment with DEX (with bFGF in the last 24 h), followed by SFM for 60 h and final addition for 24 h with growth factors alone or two of them together. Western blot analysis data showed a marked GFAP expression in cultures submitted to Condition I comparing results to untreated or treated controls. VIM expression was instead significantly reduced after GFs addition in the last 24 h of 60 h DEX treatment, respect to control DEX-pretreated ones. Referring data to untreated controls, VIM expression was significantly enhanced after GFs addition. GFAP showed also a significant increase in astrocytes submitted to Condition II, respect to untreated or treated control cultures. VIM expression was up and down regulated under Condition II. Collectively, our findings evidence an interactive dialogue between GFs and DEX in astroglial cultures, co-pretreated with DEX and bFGF, regulating cytoskeletal network under stressful conditions.

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“…INS and IGF-I are powerful mitogenic and trophic factors for both neurons and astrocytes [7][8][9]. EGF stimulates proliferation and differentiation of cultured rat primary astrocytes [10][11][12][13][14][15][16][17] and acts synergically with IGF-I [18]. Growth factors elicit their biological effects by binding to plasmalemmal tyrosine kinase receptors [19].…”

Section: Introductionmentioning

confidence: 98%

Astroglial-Conditioned Media and Growth Factors Modulate Proliferation and Differentiation of Astrocytes in Primary Culture

et al. 2006

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Astroglial conditioned media (ACM) influence the development and maturation of cultured nerve cells and modulate neuron-glia interaction. To clarify mechanisms of astroglial cell proliferation/differentiation in culture, incorporation of [methyl-3H]-thymidine or [5,6-3H]-uridine in cultured astrocytes was assessed. Cultures were pre-treated with epidermal growth factor (EGF), insulin (INS), insulin-like growth factor-I (IGF-I), and basic fibroblast growth factor (bFGF) and subsequently with ACM. DNA labeling revealed a marked stimulatory effect of ACM from 15 days in vitro (DIV) cultures in 30 DIV astrocytes after 12 h pre-treatment with growth factors. The main effects were found after INS or EGF pre-treatment in 30 DIV cultures. ACM collected from 15 or 60 or 90 DIV increased RNA labeling of 15 and 30 DIV astrocyte cultures, being the highest value that of 30 DIV cultures added with ACM from 90 DIV. The findings of increased DNA labeling after EGF or INS pre-treatment in 30 DIV cultures, followed by addition of ACM from 15 DIV cultures, suggest that these phenomena may depend by extra cellular signal-regulated kinase 1 (ERK1) activation.

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Effect of epidermal growth factor on the labeling of the various RNA species and of nuclear proteins in primary rat astroglial cell cultures

Cited by 27 publications

References 34 publications

Effect of growth factors and steroids on transglutaminase activity and expression in primary astroglial cell cultures

Effect of growth factors and steroids on transglutaminase activity and expression in primary astroglial cell cultures

Growth Factors and Steroid Mediated Regulation of Cytoskeletal Protein Expression in Serum-Deprived Primary Astrocyte Cultures

Astroglial-Conditioned Media and Growth Factors Modulate Proliferation and Differentiation of Astrocytes in Primary Culture

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