Effect of epidermal growth factor on follicle-stimulating hormone-induced proliferation of granulosa cells from chicken prehierarchical follicles

Abstract

Abstract:The development of ovarian follicular cells is controlled by multiple circulating and local hormones and factors, including follicle-stimulating hormone (FSH) and epidermal growth factor (EGF). In this study, the stagespecific effect of EGF on FSH-induced proliferation of granulosa cells was evaluated in the ovarian follicles of egg-laying chickens. Results showed that EGF and its receptor (EGFR) mRNAs displayed a high expression in granulosa cells from the prehierarchical follicles, including the large white follicle (LWF) and small yellow follicle (SYF), and thereafter the expression decreased markedly to the stage of the largest preovulatory follicle. SYF represents a turning point of EGF/EGFR mRNA expression during follicle selection. Subsequently the granulosa cells from SYF were cultured to reveal the mediation of EGF in FSH action. Cell proliferation was remarkably increased by treatment with either EGF or FSH (0.1-100 ng/ml). This result was confirmed by elevated proliferating cell nuclear antigen (PCNA) expression and decreased cell apoptosis. Furthermore, EGF-induced cell proliferation was accompanied by increased mRNA expressions of EGFR, FSH receptor, and the cell cycle-regulating genes (cyclins D1 and E1, cyclin-dependent kinases 2 and 6) as well as decreased expression of luteinizing hormone receptor mRNA. However, the EGF or FSH-elicited effect was reversed by simultaneous treatment with an EGFR inhibitor AG1478. In conclusion, EGF and EGFR expressions manifested stage-specific changes during follicular development and EGF mediated FSH-induced cell proliferation and retarded cell differentiation in the prehierarchical follicles. These expressions thus stimulated follicular growth before selection in the egg-laying chicken.

Effects of Vitamin E Supplementation on Serum Hormones and Gene Expression of Anti-Season Breeding Xingguo Grey Geese (Anser Cygnoides)

“…The literature on Hardy et al proved that FSH was essential for pre-antral follicle growth and function in mice (Hardy et al, 2017). In poultry, FSH treatment increased the embryonic chicken ovarian germ cell number, granulosa cells number and reduced cell apoptosis in different stage of follicles (Lin et al, 2011). In this study, the increase of FSH and LH in geese may induce follicular development and ovulation which may result in the increase of egg production of goose.…”

Fibroblast growth factor 9 (FGF9) regulation of cyclin D1 and cyclin-dependent kinase-4 in ovarian granulosa and theca cells of cattle

“…The only study characterizing CCND1 mRNA in bovine GC reported no change during an estrous cycle, while both CCND2 and CCND3 mRNA expression differed among phases of the estrous cycle (Shimizu et al, 2013). However, CCND1 mRNA is induced by GDF9 in human GC (Huang et al, 2009) and by EGF, FGF2 and ginsenosides in chicken GC (Tan et al, 2010; Lin et al, 2011; 2012). In endometrial tissue, FGF9 induces CCND1 (Schwartz et al, 2003; Wing et al, 2005; Chan et al, 2012).…”

CO-029 is overexpressed in gastric cancer and mediates the effects of EGF on gastric cancer cell proliferation and invasion

“…Epidermal growth factor (EGF) is a peptide of 53 amino acid residues, it binds to the epidermal growth factor receptor (EGFR) and initiates the cascade of intracellular signaling pathways. Therefore, EGF is involved in a variety of physiological and pathologic processes, including cell proliferation, apoptosis, migration, survival and angiogenesis (21)(22)(23). However, the potential molecular mechanisms underlying the effect of EGF on gastric cancer cell growth have not yet been fully elucidated.…”