Effect of epibrassinolide on tyrosine phosphorylation of the calvin cycle enzymes

Fedina, E. O.; Karimova, F. G.; Tarchevsky, I. A.; Toropygin, I. Yu.; Хрипач, В. А.

doi:10.1134/s1021443708020052

Cited by 20 publications

(9 citation statements)

References 19 publications

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“…BRs not only affect both cell division and cell elongation, having thus a significant influence on growth and development (Clouse and Sasse 1998;Haubrick and Assmann 2006;Kartal et al 2009;Rao et al 2002;Sasse 2003), but participate also in the induction of the differentiation of plant vascular system (Clouse and Sasse 1998;Sasse 2003), play an important role in the regulation of senescence (Clouse and Sasse 1998;Rao et al 2002), induce the pollen tube growth (Clouse and Sasse 1998), affect seed germination (Haubrick and Assmann 2006;Ö zdemir et al 2004;Rao et al 2002;Sharma and Bhardwaj 2007;Vardhini and Rao 2003) and induce the formation of adventitious roots (Rao et al 2002). They are also involved in the regulation of cell wall biosynthesis (Sasse 2003), activation of proton pumps in cellular membranes (Cerrana et al 1984), they enhance DNA and RNA levels (Vardhini and Rao 1998;Swamy and Rao 2008) and influence the phosphorylation of proteins (Fedina et al 2008). In plants subjected to stress conditions, BRs affect (positively or negatively) the activity of antioxidant enzymes, synthesis of various osmoprotectants, synthesis of heat shock proteins, the fatty acid composition of membrane lipids, etc.…”

Section: Introductionmentioning

confidence: 98%

The effect of brassinosteroids on the morphology, development and yield of field-grown maize

et al. 2010

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The response of two field-grown inbred lines of maize (Zea mays L.) and their F1 hybrid to the application of 10 -8 -10 -14 M solutions of 24-epibrassinolide or synthetic androstane analogue of castasterone in V3/4 and V6/ 7 developmental stages was followed during the vegetative and early reproductive phases of plant development. Brassinosteroids (BRs) significantly affected (either positively or negatively, depending on the genotype and the developmental stage they were applied) the height of plants during the early weeks after their application, but not the final plant height nor the number of leaves. Spraying of plants with BRs in V3/4 developmental stage usually also increased the length of the 7th to 10th leaf, whereas the application in V6/7 developmental stage had the opposite effect. The beginning of the reproductive phase of plant development and the course of flowering was strongly influenced by the application of BRs. Treatment of plants in V3/4 stage delayed and treatment of plants in V6/7 stage advanced the dates of anthesis and silking, regardless of the type of BR used, its concentration or plant genotype. The influence of BRs on the development of the secondary ear was the least pronounced in the F1 hybrid; in both inbred lines it strongly depended on the concentrations of BRs used. Various yield parameters were also affected by treatment of plants with BRs, but this effect depended on the developmental stage during which the application of BRs occured, the plant genotype, the type of BR and its concentration.

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Section: Introductionmentioning

confidence: 98%

The effect of brassinosteroids on the morphology, development and yield of field-grown maize

et al. 2010

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“…However, a link with BR signaling is established 3 and it is intriguing that earlier studies with pea (Pisum sativum L.) reported that exogenous BL treatment increased the apparent Tyr phosphorylation of several leaf proteins. 32 How Tyr phosphorylation of downstream components might be affected by BR signaling certainly emerges as an interesting aspect to pursue in future studies.…”

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confidence: 99%

Functional importance of BAK1 tyrosine phosphorylation in vivo

Clouse

2011

Plant Signaling & Behavior

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“…Isoelectrofocusing of proteins was performed in the Protean IEF Cell (Bio‐Rad) as described earlier (Fedina et al . 2008). After isoelectrofocusing, strips were incubated for 15 min in equilibration buffer containing 6 M urea, 2% SDS, 30% glycerol, 50 m m Tris buffer, trace amounts of bromophenol blue and 2% DTT.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, studies on the effect of 24‐epibrassinolide (EBR) on the tyrosine phosphoproteome using mass spectrometry analysis resulted in identification of enzymes involved in the dark reactions of photosynthesis in leaves of pea plants (Fedina et al . 2008).…”

Section: Introductionmentioning

confidence: 99%

24‐epibrassinolide‐induced growth promotion of wheat seedlings is associated with changes in the proteome and tyrosine phosphoproteome

et al. 2021

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Brassinosteroids (BRs) represent a unique class of steroidal plant hormones that display pronounced growth‐promoting activity at very low concentrations. Although many efforts have been made to characterize the molecular basis of BR action, little is known about the mechanisms behind the growth‐promoting effect of BRs at protein level. Proteomic analysis of response to the steroid plant hormone 24‐epibrassinolide (EBR) in wheat seedling shoots (Triticum aestivum L.) was performed using two‐dimensional electrophoresis (2‐DE) and immunoblotting with highly specific antibodies (PY20) to phosphotyrosine. EBR‐modulated proteins and phosphotyrosine polypeptides were identified using MALDI‐TOF mass spectrometry. The study revealed that EBR‐stimulated growth of wheat seedlings was accompanied by changes in the content of multiple proteins as well as in tyrosine phosphorylation of numerous polypeptides. Among them, 22 differentially accumulated proteins and 13 phosphotyrosine proteins were identified. Based on their performed functions, the identified proteins are involved in physiological processes (photosynthesis, growth, energy and amino acid metabolism) closely associated with intensification of plant metabolism. The EBR‐induced changes in protein abundance and tyrosine phosphorylation profile may contribute to growth stimulation of wheat seedlings under the action of EBR. The obtained data suggest an important role for EBR in the activation of protein metabolism underlying fundamental physiological processes, including growth promotion.

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Effect of epibrassinolide on tyrosine phosphorylation of the calvin cycle enzymes

Cited by 20 publications

References 19 publications

The effect of brassinosteroids on the morphology, development and yield of field-grown maize

The effect of brassinosteroids on the morphology, development and yield of field-grown maize

Functional importance of BAK1 tyrosine phosphorylation in vivo

24‐epibrassinolide‐induced growth promotion of wheat seedlings is associated with changes in the proteome and tyrosine phosphoproteome

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