Effect of enzyme processivity on the efficacy of a competitive chitinase inhibitor

Zakariassen, Henrik; Klemetsen, Laila; Sakuda, Shohei; Vaaje‐Kolstad, Gustav; Vårum, Kjell M.; Sørlie, Morten; Eijsink, Vincent G. H.

doi:10.1016/j.carbpol.2010.05.051

Cited by 11 publications

(6 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the dissociation of the CBM is slow, the k off for the total dissociation of enzyme may underestimate the k off for the dissociation from the active site, and P Intr is overestimated. The importance of aromatic residues in the CBM of ChiA (Trp 33 and Trp or Trp 275 to Ala had only moderate effects on binding to ␤-chitin (45). The rates of the production of IRGs on reduced ␣-chitin measured here also suggest a significant contribution of the CBM in the total dissociation of the enzyme.…”

Section: Discussionmentioning

confidence: 54%

Slow Off-rates and Strong Product Binding Are Required for Processivity and Efficient Degradation of Recalcitrant Chitin by Family 18 Chitinases

Kurašin

Kuusk

et al. 2015

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background:The role of slow off-rates of processive enzymes acting on recalcitrant polysaccharides is poorly understood. Results: Chitinase variants with high off-rates and weak product binding were inefficient in degradation of recalcitrant chitin. Conclusion: Slow off-rates and strong product binding are required for high efficiency and processivity. Significance: Knowledge of determinants of processivity aids to design better enzymes.

show abstract

Section: Discussionmentioning

confidence: 54%

Slow Off-rates and Strong Product Binding Are Required for Processivity and Efficient Degradation of Recalcitrant Chitin by Family 18 Chitinases

Kurašin

Kuusk

et al. 2015

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Enzyme binding to β-chitin nanofibers was assessed as described by Zakariassen et al [22]. Vc CDA was diluted to various concentrations (0–20 µM) in Tris-HCl (25 mM, pH 8.0) and 0.1 mM CoCl 2 .…”

Section: Methodsmentioning

confidence: 99%

Can we make Chitosan by Enzymatic Deacetylation of Chitin?

et al. 2019

Self Cite

View full text Add to dashboard Cite

Chitin, an insoluble linear polymer of β-1,4-N-acetyl-d-glucosamine (GlcNAc; A), can be converted to chitosan, a soluble heteropolymer of GlcNAc and d-glucosamine (GlcN; D) residues, by partial deacetylation. In nature, deacetylation of chitin is catalyzed by enzymes called chitin deacetylases (CDA) and it has been proposed that CDAs could be used to produce chitosan. In this work, we show that CDAs can remove up to approximately 10% of N-acetyl groups from two different (α and β) chitin nanofibers, but cannot produce chitosan.

show abstract

“…During insect growth and development, the cuticle and PM must be degraded periodically and then be replaced in order to allow for growth, maturation and repair (Zakariassen et al ., 2010; Zhang et al ., 2011). One important characteristic of the epidermis is that two closely related processes (the degradation and synthesis of chitin) occur almost simultaneously during metamorphosis (Gu et al ., 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Chitin is a polysaccharide long-chain polymer comprised of β-(1,4)-N-acetylglucosamine residues and is a vital component of the insect cuticle and peritrophic membrane (PM) ( Kramer et al ., 1995 ). During insect growth and development, the cuticle and PM must be degraded periodically and then be replaced in order to allow for growth, maturation and repair ( Zakariassen et al ., 2010 ; Zhang et al ., 2011 ). One important characteristic of the epidermis is that two closely related processes (the degradation and synthesis of chitin) occur almost simultaneously during metamorphosis ( Gu et al ., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Sequencing and de novo assembly of the Asian gypsy moth transcriptome using the Illumina platform

et al. 2016

View full text Add to dashboard Cite

The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken. We generated 37,750,380 high-quality reads and assembled them into contigs. A total of 37,098 unigenes were identified, of which 15,901 were annotated in the NCBI non-redundant protein database and 9,613 were annotated in the Swiss-Prot database. We mapped 4,329 unigenes onto 317 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. Chitin metabolism unigenes were found in the transcriptome and the data indicated that a variety of enzymes was involved in chitin catabolic and biosynthetic pathways.

show abstract

Effect of enzyme processivity on the efficacy of a competitive chitinase inhibitor

Cited by 11 publications

References 52 publications

Slow Off-rates and Strong Product Binding Are Required for Processivity and Efficient Degradation of Recalcitrant Chitin by Family 18 Chitinases

Slow Off-rates and Strong Product Binding Are Required for Processivity and Efficient Degradation of Recalcitrant Chitin by Family 18 Chitinases

Can we make Chitosan by Enzymatic Deacetylation of Chitin?

Sequencing and de novo assembly of the Asian gypsy moth transcriptome using the Illumina platform

Contact Info

Product

Resources

About