Effect of Enzyme Inhibitors on Terpene Trilactones Biosynthesis and Gene Expression Profiling in <i>Ginkgo biloba</i> Cultured Cells

Chen, Lijia; Tong, Hui; Wang, Mingxuan; Zhu, Jianhua; Zi, Jiachen; Song, Liyan; Yu, Rongmin

doi:10.1177/1934578x1501001205

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Ginkgolides and bilobalide, which are collectively called terpene trilactones, are the most unique components of G. biloba extracts and have been linked to resistance to fungi (Pan et al, 2016) and possibly to endophytic bacteria (Yang et al, 2015). The biosynthesis of bilobalide was highly correlated with the mevalonic acid pathway, and the biosynthesis of ginkgolides was highly correlated with the methylerythritol 4-phosphate pathway (Chen et al, 2015). Most structural genes of the terpene trilactone biosynthetic pathway in G. biloba, including DXS (Gong et al, 2006;Kim et al, 2006a), DXR (Gong et al, 2005), MECT (Kim et al, 2006b), CMK (Kim et al, 2008a), MECS (Kim et al, 2006c), HDS (Kim and Kim, 2009), HDR (Kim et al, 2008b;Lu et al, 2008), FPS (Wang et al, 2004), GGPPS (Liao et al, 2004), LPS (Schepmann et al, 2001), HMGR (Shen et al, 2016), MVD (Pang et al, 2006), AACT and MVK (Chen et al, 2017), have been isolated.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of WRKY Family Genes and Analysis of Their Expression in Response to Abiotic Stress in Ginkgo biloba L.

Cheng

Liu

Liao

et al. 2019

Not Bot Horti Agrobo

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Ginkgo biloba is widely planted, and the extracts of leaves contain flavonoids, terpene esters and other medicinal active ingredients. WRKY proteins are a large transcription factor family in plants, which play an important role in the regulation of plant secondary metabolism and development, as well as the response to biotic and abiotic stress. In our study, we identified 40 genes with conserved WRKY motifs in the G. biloba genome and classified into groups I (groups I-N and -C), II (groups IIa, b, c, d, and e), and III, which include 12, 26, and 2 GbWRKY genes, respectively. Meanwhile, the expression patterns of 10 GbWRKY (GbWRKY2, GbWRKY3, GbWRKY5, GbWRKY7, GbWRKY11, GbWRKY15, GbWRKY23, GbWRKY29, GbWRKY31, GbWRKY32) under different tissue and abiotic stress conditions were analyzed. Under stress treatment, the expression patterns of 10 WRKY genes were changed. 10 ginkgo WRKY transcription factors were induced by ETH and SA, but there are two different induced response modes. The expression of 10 WRKY genes was inhibited under low temperature, high temperature and MeJA hormone induction. Most WRKY genes were up-regulated under the induction of high salt and ABA. GbWRKYs were differentially expressed in various tissues after abiotic stress and plant hormone treatments, thereby indicating their possible roles in biological processes and abiotic stress tolerance and adaptation. Our results provided insight into the genome-wide identification of GbWRKYs, as well as their differential responses to stresses and hormones. These data can also be utilized to identify potential molecular targets to confer tolerance to various stresses in G. biloba. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of WRKY Family Genes and Analysis of Their Expression in Response to Abiotic Stress in Ginkgo biloba L.

Cheng

Liu

Liao

et al. 2019

Not Bot Horti Agrobo

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“…In addition, dry cultured cells were extracted sonically with 3 times volumes of methanol, then the ultrasonic extraction was concentrated to dryness under vacuum and then dissolved in a mixture of ethyl acetate and water (1:1, v/v), and partitioning with ethyl acetate (v/v) 3 times, combined and concentrated the ethyl acetate extracts to dryness ultimately. At last, the residues of cells and medium were amalgamated and dissolved in 1.0 mL methanol and then analyzed by HPLC-ELSD [14,15]. The present study utilized the 1260 HPLC instrument (Agilent Technologies, US) equipped with an evaporative light-scattering detector (ELSD, Alltech Technologies, US) to measure the quantification of GKs and BB.…”

Section: Lp Treatment Dry Weight Measurement Of the G Biloba Cellsmentioning

confidence: 99%

Effect of Levopimaradiene on Terpene Trilactones Biosynthesis and Gene Expression Profiling in Ginkgo biloba Cells

Zhu

Wang

Qian

et al. 2017

Natural Product Communications

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Ginkgolides (GKs) and Bilobalide (BB) are rare terpene trilactones obtained from Ginkgo biloba, but their biosynthetic pathway is still unclear. In this paper, effects of levopimaradiene (LP) on increasing the production of terpene trilactones of G. biloba dedifferentiated cells (DDCs) and cambial meristematic cells (CMCs) were reported. The productions of ginkgolide A (GA) and ginkgolide B (GB) were 1.61 and 1.32 folds larger than that of the control groups when G. biloba DDCs was treated with LP, and the productions of ginkgolide C (GC) and BB reached 234 and 161 μg L−1 after treated with LP for 60 h. The production of GA, GB, GC and BB was 2.03, 1.43, 1.22 and 1.19 folds larger than that of the control groups in LP-treated CMCs groups. The results demonstrated that BB could be produced from the methylerythritol 4-phosphate (MEP) pathway. qRT-PCR experiments showed that LP was a significant precursor manipulated the biosynthesis of terpene trilactones via affecting the transcript levels of several related genes in the MEP pathway.

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Effect of Enzyme Inhibitors on Terpene Trilactones Biosynthesis and Gene Expression Profiling in Ginkgo biloba Cultured Cells

Cited by 2 publications

References 15 publications

Genome-Wide Identification of WRKY Family Genes and Analysis of Their Expression in Response to Abiotic Stress in Ginkgo biloba L.

Genome-Wide Identification of WRKY Family Genes and Analysis of Their Expression in Response to Abiotic Stress in Ginkgo biloba L.

Effect of Levopimaradiene on Terpene Trilactones Biosynthesis and Gene Expression Profiling in Ginkgo biloba Cells

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