Effect of Enzyme Concentration on Quality of Apple Pomace Pectin

Chandel, Vinay; Vaidya, Devina; Gupta, Anil; Kaushal, Manisha; Verma, Anil Kumar

doi:10.20546/ijcmas.2019.801.298

Cited by 1 publication

(1 citation statement)

References 14 publications

(14 reference statements)

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“…These effects can be attributable to a reduction in the degree of polymerization of the alginate molecules as a result of the heat treatments (43) . Fortunately, pectin was reported to be autoclavable and stable when heated at 121 for 60 min (44) . However, whey protein, which is mainly composed of globulin, will inevitably form aggregation.…”

Section: Encapsulation Efficiency (Ee %)mentioning

confidence: 99%

Encapsulation of Lactobacillus rhamnosus in alginate, pectin and whey protein targeting colon cancer

Hamid,

El-Nikhely,

Hussein

et al. 2024

Journal of Advanced Pharmaceutical Sciences

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The encapsulation of probiotic cells in prebiotic polymers is one of the most effective techniques to maintain probiotic viability during gastrointestinal transit and storage. The purpose of this study was to fabricate a suitable colonic delivery system for Lactobacillus rhamnosus using alginate, pectin, and whey protein for oral administration. The effects of biopolymers at different concentrations and ratios under various sterilization temperatures on Lactobacillus rhamnosus viability were evaluated. The microbeads were evaluated for entrapment efficiency, shape, and morphology by SEM. The viability of free and encapsulated Lactobacillus rhamnosus in simulated gastric fluids was also evaluated. Moreover, MTT assay was implemented to determine the cytotoxic effect of Lactobacillus rhamnosus microbeads using the colon cancer cell line Caco-2. Different signaling pathways in the Caco-2 cell line were also studied. The results showed that the formulae produced from polymers sterilized at low temperature had the highest entrapment efficiency and viability for L. rhamnosus. The symbiotic effect of probiotics and prebiotics was observed mainly after incubation in simulated colon fluid for 4 h and 24 h. The mixture of components of the microbeads M12 showed an anticancer effect on Caco-2 by upregulation of Bax and caspase-3 and downregulation of Bcl-2.

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Section: Encapsulation Efficiency (Ee %)mentioning

confidence: 99%