Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane

Jamil, N.; Man, Rohaida Che; Suhaimi, Syahida; Shaarani, Shalyda Md; Arshad, Zatul Iffah Mohd; Mudalip, Siti Kholijah Abdul; Sulaiman, Siti Zubaidah

doi:10.1016/j.matpr.2018.07.065

Cited by 13 publications

(8 citation statements)

References 34 publications

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“…However, the decrease in expressed activity with increasing enzyme concentration can be attributed to the oversaturation of the enzyme over the support surface. Moreover, high enzyme concentration clustered on the surface of the support can reduce enzyme activity, owing to limited active site accessibility and denaturation of the enzyme 114 – 117 . Furthermore, the immobilization protocol might distort the enzyme, when there are multi-interactions between the enzyme and the support.…”

Section: Resultsmentioning

confidence: 99%

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Vasić

Knez

Leitgeb

2020

Sci Rep

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A novel method is described for the immobilization of alcohol dehydrogenase (ADH) from Saccharomyces cerevisiae onto carboxymethyl dextran (CMD) coated magnetic nanoparticles (CMD-MNPs) activated with epoxy groups, using epichlorohydrin (EClH). EClH was used as an activating agent to bind ADH molecules on the surface of CMD-MNPs. Optimal immobilization conditions (activating agent concentration, temperature, rotation speed, medium pH, immobilization time and enzyme concentration) were set to obtain the highest expressed activity of the immobilized enzyme. ADH that was immobilized onto epoxy-activated CMD-MNPs (ADH-CMD-MNPs) maintained 90% of the expressed activity. Thermal stability of ADH-CMD-MNPS after 24 h at 20 °C and 40 °C yielded 79% and 80% of initial activity, respectively, while soluble enzyme activity was only 19% at 20 °C and the enzyme was non-active at 40 °C. Expressed activity of ADH-CMD-MNPs after 21 days of storage at 4 °C was 75%. Kinetic parameters (KM, vmax) of soluble and immobilized ADH were determined, resulting in 125 mM and 1.2 µmol/min for soluble ADH, and in 73 mM and 4.7 µmol/min for immobilized ADH.

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Section: Resultsmentioning

confidence: 99%

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Vasić

Knez

Leitgeb

2020

Sci Rep

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“…This is due to the fact that at high temperatures, the active site of the enzyme changes shape, making it less complementary to the shape of the substrate, hence, its ability to catalyze is reduced. Finally, the enzyme is denatured and no longer functions [24].…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Characterization of N-Methyl Fatty Hydroxamic Acids from Ketapang Seed Oil Catalyzed by Lipase

Suhendra¹,

Gunawan²,

Hajidi³

2019

Molecules

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N-methyl fatty hydroxamic acid (N-MFHA), which is a derivative of hydroxamic acid (HA), was synthesized from ketapang seed oil (Terminalia catappa L.). In general, HAs have wide applications due to their chelating properties and biological activities. N-MFHAs were synthesized using immobilized lipase (Lipozyme TL IM) in biphasic medium which was the ketapang seed oil dissolved in hexane and N-methylhydroxylamine dissolved in water. The products were characterized through color testing and FT-IR spectroscopy after purification. Various factors affecting the enzyme activity investigated in the study included the effect of incubation time, the amount of lipase used, and the temperature. On the basis of the results, the optimum conditions for the synthesis of N-MFHA obtained are 25 h of incubation time, a temperature of 40 °C, and a ratio of 1:100 for the amount of enzyme (g)/oil (g). At the optimum conditions of the reaction, 59.7% of the oils were converted to N-MFHA.

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“…Hollow fibre membranes can be applied in various fields, including water treatment, medicine, pharmaceutical, and food processing due to their unique structures [42]. Hollow fibre membranes also offer high mechanical strength, a high surface-tovolume ratio, operational durability, non-toxicity, excellent chemical resistance, and biodegradability [43].…”

Section: Mechanical Containment Behind a Barriermentioning

confidence: 99%

Cell Immobilization for Cyclodextrin Production: Mini Review

Baharudin¹,

Man²,

Manas

et al. 2023

Jurnal Teknologi

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Cell immobilization has been applied in various industries, including chemical manufacturing, food, pharmaceutical, and textile. Recently, innovations in cell immobilization techniques and support materials have been put forward for application in high value-added chemical biosynthesis, such as cyclodextrin (CD). The techniques, support materials, and process parameters of cell immobilization play important roles in achieving high CD yield. This review should help one choose the correct cell immobilization technique and support for a CD biosynthesis setup. Previously, CD biosynthesis utilized free cells, even though they present difficulties such as the low product yield, cell lysis, unstable plasmid, and non-reusable cells. This review highlights how the problems that arise from free-cell bioreactors could be mitigated by cell immobilization. The process conditions of cell immobilization for CD production are also presented.

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Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane

Cited by 13 publications

References 34 publications

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Synthesis and Characterization of N-Methyl Fatty Hydroxamic Acids from Ketapang Seed Oil Catalyzed by Lipase

Cell Immobilization for Cyclodextrin Production: Mini Review

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