Effect of enzyme assisted extraction on quality and yield of volatile oil from black pepper and cardamom

Enzyme-assisted extraction of bioactive compounds from bay leaves (Laurus nobilis L.)

“…The presence of oxidases in the enzyme preparation is suggested too. The positive effect of enzymes on the release of oxygenated components was also reported for cardamom (Chandran et al, 2012), F. forsythiae (Jiao et al, 2012), thyme and rosemary (Hosni et al, 2013). In the former species, it was found that the use of Lumicellulase (a mixture of cellulase, ␤-glucanase, petinase and xylanase) caused a reduction of hydrocarbons, whereas, it significantly improve the release of oxygenated compounds, leading hence to increased O/H ratio in enzyme-treated cardamom.…”

“…In contrast, pre-treatment of cumin seeds with cellulase and Viscozyme (commercial mixture of enzymes including cellulase, hemicellulase, pectinase, arabinase and xylanase) afforded enriched hydrocarbons essential oils as reflected in the lower O/H ratios (0.53 and 0.44 for cellulase and Viscozyme treated samples, versus 0.58 fro the untreated seeds) (Sowbhagya et al, 2011). Application of Lumicellulase to black pepper resulted in significant increase of (Chandran et al, 2012). At this point it seems that the release of volatile compounds from enzyme-treated samples is dependent on the plant species/organs, enzymes used, source of enzymes, and duration of treatment.…”

Effect of Enzymatic, Ultrasound, and Reflux Extraction Pretreatments on the Yield and Chemical Composition of Essential Oils

“…Also, the essential oil composition obtained after different pretreatments was comparable to the essential oils obtained by direct hydrodistillation. Other studies had also shown that enzymatic and ultrasonic pretreatments did not affect the overall composition of the oil [ 11 , 15 , 16 , 17 , 21 , 22 , 26 ], although some authors reported that the quantities of individual major components varied significantly in relation to the extraction technique used [ 1 , 2 , 12 , 13 , 14 , 19 , 24 ]. For instance, Boulila et al [ 19 ] reported that enzyme pretreatment of bay laurel leaves resulted in increased concentration of oxygenated monoterpenes, and explained such effect by a possible increase in oxidation after cell wall disruption or the presence of oxidases in the enzyme preparation.…”

“…Enzymes, like cellulase, xylanase, and pectinase, can degrade or disrupt cell wall components, thus enabling better release and more efficient extraction of bioactive compounds from plants and improving the bioactive content of essential oils and extracts [ 2 , 18 , 19 , 20 , 21 , 22 ]. However, while some authors reported that enzyme-assisted extraction enhanced essential oil yield and improved their chemical composition [ 2 , 19 , 21 , 22 , 23 , 24 , 25 , 26 ], others did not demonstrate such effect [ 1 ], indicating that further research of enzyme application in plant extractions is necessary.…”

The Effect of Enzymatic Pre-Treatment in Agarwood Oil Extraction

“…A study done in a uniform concentration to compare the effect of different enzyme shows that cellulase afforded the highest yield of essential oil of Fructus forsythia [17] and is consistent with our findings. The enzyme-assisted extraction improved the efficiency of extraction attributing to the destruction of the cell wall components [18]. Boulila et al [19] in their study stated that the enzyme pre-treatment did not induce transformation of the volatile components in bay leaves (Laurus nobilis L.) but contributed to the release of some glycosidically bound volatiles which enhanced the release of phenolic compounds.…”