Effect of enzyme and substrate dosing strategies on mixing and hydrolysis of old corrugated cardboard

A discretized model for enzymatic hydrolysis of cellulose in a fed-batch process

“…The reactor diameter was 13 cm and a down pumping pitched blade impeller with a diameter of 5.9 cm was used for mixing at impeller revolution rates of 300, 400 or 500 rpm. More detailed information of the reactor system can be found in(Sotaniemi et al, 2015).Prior to starting a fed-batch experiment, the reactor was filled with 800 ml of the buffer solution and 30 g of the substrate. The reaction was started by adding enzyme solution corresponding to 5, 10 or 15 FPU g sol -1 calculated based on the total added solids.…”