Effect of enzyme-activated inhibitors on ornithine decarboxylase and growth of Botrytis cinerea

Smith, Terence A.; Barker, J. H. A.; Jung, Michael J.

doi:10.1016/0031-9422(90)85010-d

Cited by 13 publications

(4 citation statements)

References 18 publications

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“…ALP, which is similarly known as an efficient inhibitor of ODC (Bey et al, 1987), was likewise comparatively ineffective in causing reduction of Botrytis growth. DFMA and AMFMA do not inhibit Botrytis ODC in vitro (Smith et al, 1990) and ODC inhibition cannot therefore explain the growth reduction found with the arginine analogues. There are several possible reasons for this anomaly.…”

Section: Resultsmentioning

confidence: 77%

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Growth inhibition of Botrytis cinerea by compounds interfering with polyamine metabolism

Smith¹,

Barker²,

Jung³

1990

Journal of General Microbiology

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Several inhibitors of ornithine and arginine decarboxylases reduced growth of the fungus Botrytis cinerea cultured on Czapek Dox agar. Of these, the most effective were difluoromethylornithe (DFMO), dehydromonofluoromethylornithine, difluoromethylarginine and dehydromonofluoromethylarginine. The growth inhibition due to 1 mM-DFMO could be partially reversed with 1 pM-putrescine. Other compounds causing significant reversal of DFMO-mediated growth inhibition included diaminopentane (cadaverine), diaminoheptane, spermidine, 7,7-difluorospermidine, spermine, bis(2-aminoethyl)amine, 2-hydroxy-1,3-diaminopropane, monoacetylputrescine, butenediamine and aminoguanidine. Some compounds, which were relatively innocuous by themselves, increased growth inhibition due to DFMO. Notably effective compounds were methylacetylenicputrescine, aminooxyaminopropane, butynediamine, 2,2-difluoroputrescine, diacetylputrescine, methylglyoxal bis(guanylhydrazone), streptomycin, certain methylated amines, and cyclohexylamine and related compounds. Growth inhibition due to a homologous series of diguanidines [NH,C(=NH)NH(CH,),NHC(=NH)NH,] was also tested. These were especially effective when x = 12, and when x = 5 or 6. In general, the results suggest that amino-acid-based inhibitors of ornithine decarboxylase have a greater permeability than amine-based inhibitors.

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Section: Resultsmentioning

confidence: 77%

“…The best were DFMO, AMFMO, DFMA and AMFMA (Table 1). However, MAP, which is a particularly effective enzyme-activated inhibitor of ODC in rat prostate, Trypanosoma brucei brucei (Bey et al, 1987) and Botrytis cinerea (Smith et al, 1990;M. Goosey, personal communication) appeared to produce a relatively small reduction in growth (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Growth inhibition of Botrytis cinerea by compounds interfering with polyamine metabolism

Smith¹,

Barker²,

Jung³

1990

Journal of General Microbiology

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“…The fact that certain concentrations of both CHA and MGBG increased ODC activity in germinating ascospores is interesting. CHA was also found to increase the yield of ODC several-fold when added to the culture medium of Neurospora crassa (DiGangi et al, 1987) and Botrytis cinerea (Smith et al, 1990). Given that these inhibitors decreased cellular SPD levels, it is tempting to speculate that a SPD-mediated mechanism for the regulation of ODC activity/levels operates in this fungus.…”

Section: Discussionmentioning

confidence: 96%

Polyamine metabolism during the germination of Sclerotinia sclerotiorum ascospores and its relation with host infection

et al. 2004

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Summary• Polyamine biosynthesis inhibitors were used to study polyamine metabolism during the germination of Sclerotinia sclerotiorum ascospores, and to evaluate the potential of polyamine biosynthesis inhibition for the control of ascospore-borne diseases in plants.• The effects of inhibitors on ascospore germination, free polyamine levels, ornithine decarboxylase activity and development of disease symptoms on tobacco ( Nicotiana tabacum ) leaf discs inoculated with ascospores were determined.• α -Difluoromethylornithine inhibited ornithine decarboxylase and decreased free spermidine levels, but had no effect on ascospore germination. Both, the spermidine synthase inhibitor cyclohexylamine and the S -adenosyl-methionine decarboxylase inhibitor methylglyoxal bis -[guanyl hydrazone] decreased free spermidine levels, but only the latter inhibited ascospore germination, at concentrations of 5 m M or higher. Lesion development on leaf discs was reduced by cyclohexylamine and methylglyoxal bis -[guanyl hydrazone], but not by α -difluoromethylornithine. In the absence of inhibitors, dormant ascospores contained higher polyamine levels than mycelium.• Ascospore germination did not depend on ornithine decarboxylase activity and inhibitors of this enzyme will probably have a limited potential for the control of ascospore-borne plant diseases. On the contrary, spermidine synthase and S -adenosylmethionine decarboxylase could be more suitable targets for fungicidal action. The relative insensitivity of ascospore germination to polyamine biosynthesis inhibitors may be caused by their high polyamine content.

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“…However, it has been proposed that this effect is the result of the conversion of DFMA into DFMO via arginase (EC 3.5.3.1, Fig. 1), an enzyme known to occur in fungi and higher plants [11–13]. Nevertheless there is some evidence for the existence of ADC activity in some fungi [14,15] and therefore, inhibition of this enzyme by DFMA could reduce fungal growth in such cases.…”

Section: Introductionmentioning

confidence: 99%

Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores

Sannazzaro

Álvarez

Menéndez

et al. 2004

FEMS Microbiology Letters

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The pathways for putrescine biosynthesis and the effects of polyamine biosynthesis inhibitors on the germination and hyphal development of Gigaspora rosea spores were investigated. Incubation of spores with different radioactive substrates demonstrated that both arginine and ornithine decarboxylase pathways participate in putrescine biosynthesis in G. rosea. Spermidine and spermine were the most abundant polyamines in this fungus. The putrescine biosynthesis inhibitors K-difluoromethylarginine and K-difluoromethylornithine, as well as the spermidine synthase inhibitor cyclohexylamine, slightly decreased polyamine levels. However, only the latter interfered with spore germination. The consequences of the use of putrescine biosynthesis inhibitors for the control of plant pathogenic fungi on the viability of G. rosea spores in soil are discussed.

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Effect of enzyme-activated inhibitors on ornithine decarboxylase and growth of Botrytis cinerea

Cited by 13 publications

References 18 publications

Growth inhibition of Botrytis cinerea by compounds interfering with polyamine metabolism

Growth inhibition of Botrytis cinerea by compounds interfering with polyamine metabolism

Polyamine metabolism during the germination of Sclerotinia sclerotiorum ascospores and its relation with host infection

Ornithine and arginine decarboxylase activities and effect of some polyamine biosynthesis inhibitors on Gigaspora rosea germinating spores

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