Effect of environmentally-relevant concentrations of nonylphenol on sexual differentiation in zebrafish: a multi-generational study

Sun, Dawei; Chen, Qi; He, Ning; Diao, Panpan; Lixing, Jia; Duan, Shunshan

doi:10.1038/srep42907

Cited by 16 publications

(10 citation statements)

References 45 publications

(46 reference statements)

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“…No incidence of testis-ova was found neither in the control group nor in 10 μgL -1 exposed fish. Sex ratio in the multigeneration exposed to NP was affected, where the proportion of females accounted for 80% (36 females) in 20 μgL −1 of NP, however, treatment with 2 μgL −1 and 200 μgL −1 did not show any significant difference from the control (Sun et al, 2017). In Contrary, rainbow trout (Oncorhynchus mykiss) exposed to 1.05 and 10.17 µg NP L -1 from the egg stage until 1 year of age did not induce testis-ova or lead to alter the sex ratios when compared with the control group (Ackermann et al, 2002).…”

Section: Sc Sc Szmentioning

confidence: 90%

“…In Nile tilapia, the mortality rate reached 40% after 7 days' exposure to 500 μgL -1 NP and 13.34% after 10 days from stopping exposure to NP (Ismail and Mahboub, 2016). In recent study carried out on zebrafish by Sun et al (2017) showed that NP can behave as a strong estrogen agonist at environmentally-relevant concentrations. The estrogenic effect of NP potentially disrupted the growth and sexual differentiation of zebrafish.…”

Section: Growth Performance and Survival Ratementioning

confidence: 99%

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Nonylphenol ethoxylate (NPE) influence on reproductive performance of male Nile tilapia, Oreochromis niloticus

Ahmed¹,

Amer²,

Osman³

2019

Egypt. J. of Aquatic Biolo. and Fish.

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The aim of the present study was to investigate the effects of xenoestrogens, like nonylphenol (NP) on growth performance and gonads development of male Nile tilapia. Fish were randomly distributed into four groups in 16 fiberglass tanks with carrying capacity of 15 fish/tank and four replicates. Fish were exposed to different concentrations of NP. The contaminations of NP were 0, 25, 50 and 100 μgL-1 administrated for 126 days. After exposure period, fish weight and survival were measured. Gonads and liver were dissected for calculating both Gonado-Somatic Index (GSI) and Hepato-Somatic Index (HSI) and processed for histological examination. At the end of the exposure period in all treated groups, the average survival rate was significantly lower than that of the control. The average body weights were ranged between 73.41 ± 1.37g-78.14 ± 6.86g for the control and 50 µg NP L-1 , respectively with no statistical significant differences. Results showed slight changes in liver weight among treated group compared to the control. A significant (P<0.05) reduction in gonads weight and GSI occurred in exposed fish compared to the control. Testicular sections from the control group appeared fully mature where, lumens and ducts were loaded with spermatozoa. However, testicular sections from NP exposed fish showed germinal epithelium degeneration, which correlated with exposure level of NP and reflected on low weight of the gonads. The presence of oocytes within the testicular tissue was pronounced, especially in high dose exposed fish. Therefore, severity of testis-ova was mild in 25 µg NP L-1 , moderate to pronounced in 50 and 100 µg NP L-1 exposed groups, respectively. From the obtained results, it could be concluded that the environmental pollutants with estrogenic activity such as nonylphenol can alter the development of gonads and disrupt reproduction of wild and farmed fish.

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Section: Sc Sc Szmentioning

confidence: 90%

Section: Growth Performance and Survival Ratementioning

confidence: 99%

Nonylphenol ethoxylate (NPE) influence on reproductive performance of male Nile tilapia, Oreochromis niloticus

Ahmed¹,

Amer²,

Osman³

2019

Egypt. J. of Aquatic Biolo. and Fish.

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“…In addition, we found significantly delayed hatching time in the F1-2 following three consecutive generations of BaP exposure group (G1F1-3), except for F3. It is possible that marine medaka becomes resistant to BaP stress after long-term exposure [21,25].…”

Section: Discussionmentioning

confidence: 99%

“…Twenty embryos (5 d pf ) per replicate were collected, homogenized and stored at -80 • C for subsequent RNA extraction, respectively. The Quantitative real-time PCR analysis was performed according to our previous study [21]. In brief, total RNA was extracted from three fish using Trizol reagent (Invitrogen).…”

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

Long-Term Exposure to Benzo[a]Pyrene Affects Sexual Differentiation and Embryos Toxicity in Three Generations of Marine Medaka (Oryzias Melastigma)

Sun

Chen

Zhu

et al. 2020

IJERPH

Self Cite

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Benzo[a]pyrene (BaP) is a common environmental disrupting chemical that can cause endocrine disorders in organisms. However, the continued interference effects of BaP on multi-generation fish needs further research. In this study, we performed different periods (G1F1-3, G2F2-3, G3F3) of BaP exposure on marine medaka. We determined the embryo toxicity, and analyzed relative reproductive genes (ERα, cyp19a and vtg1) to predict the sexual differentiation of marine medaka. The results showed that high concentrations of BaP (200 μg·L−1) significantly delayed the hatching time of embryos. Moreover, medium/high concentrations of BaP (20 and 200 μg·L−1) prolonged the sexual maturity time of marine medaka. The relative gene expression of ERα, cyp19a and vtg1 were measured at 5 dpf of embryos. We found that BaP had significantly inhibited the expression of the genes related to female fish development. Consequently, there were more males in the offspring sex ratio at BaP exposure. Overall, BaP can cause embryonic toxicity and abnormal sexual differentiation, while the expression of related reproductive genes can effectively indicate the sex ratio.

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“…The natural and synthetic steroid estrogens, 17b-estradiol (E2), and 17 a-ethinylestradiol (EE2), and alkyl phenols such as nonylphenol (NP) are among the most pervasive EDCs found in the aquatic environment (52)(53)(54)(55)(56). These chemicals are commonly found in sewage effluent discharged into rivers (57) and affect fish at concentrations, that range from 4.5 to 25 ng/L and 0.84 to 200 µg/L, respectively (58)(59)(60)(61). EE2, a primary estrogen component of most oral contraceptive pills, is discharged through municipal wastewater.…”

Section: Introductionmentioning

confidence: 99%

Experimental Approaches for Characterizing the Endocrine-Disrupting Effects of Environmental Chemicals in Fish

2021

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Increasing industrial and agricultural activities have led to a disturbing increase of pollutant discharges into the environment. Most of these pollutants can induce short-term, sustained or delayed impacts on developmental, physiological, and behavioral processes that are often regulated by the endocrine system in vertebrates, including fish, thus they are termed endocrine-disrupting chemicals (EDCs). Physiological impacts resulting from the exposure of these vertebrates to EDCs include abnormalities in growth and reproductive development, as many of the prevalent chemicals are capable of binding the receptors to sex steroid hormones. The approaches employed to investigate the action and impact of EDCs is largely dependent on the specific life history and habitat of each species, and the type of chemical that organisms are exposed to. Aquatic vertebrates, such as fish, are among the first organisms to be affected by waterborne EDCs, an attribute that has justified their wide-spread use as sentinel species. Many fish species are exposed to these chemicals in the wild, for either short or prolonged periods as larvae, adults, or both, thus, studies are typically designed to focus on either acute or chronic exposure at distinct developmental stages. The aim of this review is to provide an overview of the approaches and experimental methods commonly used to characterize the effects of some of the environmentally prevalent and emerging EDCs, including 17 α-ethinylestradiol, nonylphenol, BPA, phthalates, and arsenic; and the pervasive and potential carriers of EDCs, microplastics, on reproduction and growth. In vivo and in vitro studies are designed and employed to elucidate the direct effects of EDCs at the organismal and cellular levels, respectively. In silico approaches, on the other hand, comprise computational methods that have been more recently applied with the potential to replace extensive in vitro screening of EDCs. These approaches are discussed in light of model species, age and duration of EDC exposure.

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Effect of environmentally-relevant concentrations of nonylphenol on sexual differentiation in zebrafish: a multi-generational study

Cited by 16 publications

References 45 publications

Nonylphenol ethoxylate (NPE) influence on reproductive performance of male Nile tilapia, Oreochromis niloticus

Nonylphenol ethoxylate (NPE) influence on reproductive performance of male Nile tilapia, Oreochromis niloticus

Long-Term Exposure to Benzo[a]Pyrene Affects Sexual Differentiation and Embryos Toxicity in Three Generations of Marine Medaka (Oryzias Melastigma)

Experimental Approaches for Characterizing the Endocrine-Disrupting Effects of Environmental Chemicals in Fish

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