Effect of Environmental Stresses on Growth Pattern, Biofilm Formation and Biochemical Characteristics of Mycobacterium marinum CCUG20998

Ghasemi, Mohammad Faezi; Moslem, Mina Nasiri; Mirpour, Mirsasan

doi:10.17795/zjrms-2664

Cited by 6 publications

(7 citation statements)

References 17 publications

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“…Al-Nabulsi et al [ 20 ] showed a tendency for antibiotic resistance to increase with decreasing pH. In contrast, Faezi-Ghasemi and Kazemi [ 56 ] have found that L. monocytogenes exposed to pH 5 or osmotic stress (7% NaCl) was more sensitive to tetracycline, rifampicin, gentamicin, penicillin, ampicillin, trimethoprim-sulfamethoxazole and chloramphenicol. In our study, the effect of osmotic and acid stress on MIC values depended on the antibiotic used and strain.…”

Section: Discussionmentioning

confidence: 99%

The influence of stress factors on selected phenotypic and genotypic features of Listeria monocytogenes – a pilot study

Wiktorczyk-Kapischke,

Wałecka-Zacharska,

Korkus

et al. 2023

BMC Microbiol

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Background Listeria monocytogenes are Gram-positive rods, widespread in the environment due to their wide tolerance to changing conditions. The apilot study aimed to assess the impact of six various stresses (heat, cold, osmotic, acid, alkali, frozen) on phenotypic features: MIC of antibiotics (penicillin, ampicillin, meropenem, erythromycin, co-trimoxazole; gradient stripes), motility, ability to form a biofilm (crystal violet method) and growth rate (OD and quantitative method), expression level of sigB (stress induced regulator of genes), agrA, agrB (associated with biofilm formation) and lmo2230, lmo0596 (acid and alkali stress) (qPCR) for three strains of L. monocytogenes. Results Applied stress conditions contributed to changes in phenotypic features and expression levels of sigB, agrA, agrB, lmo2230 and lmo0596. Stress exposure increased MIC value for penicillin (ATCC 19111 - alkaline stress), ampicillin (472CC - osmotic, acid, alkaline stress), meropenem (strains: 55 C - acid, alkaline, o smotic, frozen stress; 472CC - acid, alkaline stress), erythromycin (strains: 55 C - acid stress; 472CC - acid, alkaline, osmotic stress; ATCC 19111 - osmotic, acid, alkaline, frozen stress), co-trimoxazole (strains: 55 C - acid stress; ATCC 19111 - osmotic, acid, alkaline stress). These changes, however, did not affect antibiotic susceptibility. The strain 472CC (a moderate biofilm former) increased biofilm production after exposure to all stress factors except heat and acid. The ATCC 19111 (a weak producer) formed moderate biofilm under all studied conditions except cold and frozen stress, respectively. The strain 55 C became a strong biofilm producer after exposure to cold and produced a weak biofilm in response to frozen stress. Three tested strains had lower growth rate (compared to the no stress variant) after exposure to heat stress. It has been found that the sigB transcript level increased under alkaline (472CC) stress and the agrB expression increased under cold, osmotic (55 C, 472CC), alkali and frozen (472CC) stress. In contrast, sigB transcript level decreased in response to acid and frozen stress (55 C), lmo2230 transcript level after exposure to acid and alkali stress (ATCC 19111), and lmo0596 transcript level after exposure to acid stress (ATCC 19111). Conclusions Environmental stress changes the ability to form a biofilm and the MIC values of antibiotics and affect the level of expression of selected genes, which may increase the survival and virulence of L. monocytogenes. Further research on a large L. monocytogenes population is needed to assess the molecular mechanism responsible for the correlation of antibiotic resistance, biofilm formation and resistance to stress factors.

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Section: Discussionmentioning

confidence: 99%

The influence of stress factors on selected phenotypic and genotypic features of Listeria monocytogenes – a pilot study

Wiktorczyk-Kapischke,

Wałecka-Zacharska,

Korkus

et al. 2023

BMC Microbiol

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“…The results demonstrated that food strains resistant to ciprofloxacin, nitrofurantoin and erythromycin were significantly more resistant to acidic stress than susceptible strains [34]. In contrast, Faezi-Ghasemi and Kazemi (2014) observed an increase in the susceptibility of L. monocytogenes subjected to the sublethal level of the environmental stresses (HCl, pH = 5.0) with a decreased MIC for gentamicin and rifampicin [73]. For A. baumannii, Ebinesh et al (2018) tested the influence of pH stress (acid at 3, 5, and 6 and alkaline at 9 and 10 pH for 2 h) on the antibiotic susceptibility of six strains; the exposure to acidity conferred a higher degree of resistance than alkalinity to amikacin, piperacillin, tazobactam, imipenem and meropenem.…”

Section: Food Preservatives and Decontaminantsmentioning

confidence: 92%

Antimicrobials and Food-Related Stresses as Selective Factors for Antibiotic Resistance along the Farm to Fork Continuum

2021

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Antimicrobial resistance (AMR) is a global problem and there has been growing concern associated with its widespread along the animal–human–environment interface. The farm-to-fork continuum was highlighted as a possible reservoir of AMR, and a hotspot for the emergence and spread of AMR. However, the extent of the role of non-antibiotic antimicrobials and other food-related stresses as selective factors is still in need of clarification. This review addresses the use of non-antibiotic stressors, such as antimicrobials, food-processing treatments, or even novel approaches to ensure food safety, as potential drivers for resistance to clinically relevant antibiotics. The co-selection and cross-adaptation events are covered, which may induce a decreased susceptibility of foodborne bacteria to antibiotics. Although the available studies address the complexity involved in these phenomena, further studies are needed to help better understand the real risk of using food-chain-related stressors, and possibly to allow the establishment of early warnings of potential resistance mechanisms.

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“…Exposure to environmental stress influences cell morphology, antimicrobial resistance (AMR), pathogenicity and virulence of L. monocytogenes through the expression of a number of genes ( Matereke and Okoh, 2020 ). More, sublethal stress may contribute to stress adaptation (resistance to higher levels of the same stress factor) or cross-resistance (resistance to other stress factors) ( Faezi-Ghasemi and Kazemi, 2015 ). The pathogen has developed a number of mechanisms that allow adaptation to stressful conditions.…”

Section: Pathogenicity and Adaptation To Stress Conditionsmentioning

confidence: 99%

“…According to the crystal structure, the LLO consists of four distinct domains (D1—D4), which play a different role in the functioning of the LLO ( Köster et al, 2014 ). The D1 domain possesses a structural motif in the C-terminal region, crucial for membrane binding and the cytotoxic activity of the LLO ( Faezi-Ghasemi and Kazemi, 2015 ; Materake and Okoh, 2020 ). In turn, the D2 domain is the sequence that joins D1 to D4 via a glycine linker ( Rosado et al, 2008 ; Köster et al, 2014 ).…”

Section: Patgogenicity Islandsmentioning

confidence: 99%

Genomic and pathogenicity islands of Listeria monocytogenes—overview of selected aspects

Wiktorczyk-Kapischke

Skowron

Wałecka-Zacharska

2023

Front. Mol. Biosci.

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Listeria monocytogenes causes listeriosis, a disease characterized by a high mortality rate (up to 30%). Since the pathogen is highly tolerant to changing conditions (high and low temperature, wide pH range, low availability of nutrients), it is widespread in the environment, e.g., water, soil, or food. L. monocytogenes possess a number of genes that determine its high virulence potential, i.e., genes involved in the intracellular cycle (e.g., prfA, hly, plcA, plcB, inlA, inlB), response to stress conditions (e.g., sigB, gadA, caspD, clpB, lmo1138), biofilm formation (e.g., agr, luxS), or resistance to disinfectants (e.g., emrELm, bcrABC, mdrL). Some genes are organized into genomic and pathogenicity islands. The islands LIPI-1 and LIPI-3 contain genes related to the infectious life cycle and survival in the food processing environment, while LGI-1 and LGI-2 potentially ensure survival and durability in the production environment. Researchers constantly have been searching for new genes determining the virulence of L. monocytogenes. Understanding the virulence potential of L. monocytogenes is an important element of public health protection, as highly pathogenic strains may be associated with outbreaks and the severity of listeriosis. This review summarizes the selected aspects of L. monocytogenes genomic and pathogenicity islands, and the importance of whole genome sequencing for epidemiological purposes.

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Effect of Environmental Stresses on Growth Pattern, Biofilm Formation and Biochemical Characteristics of Mycobacterium marinum CCUG20998

Cited by 6 publications

References 17 publications

The influence of stress factors on selected phenotypic and genotypic features of Listeria monocytogenes – a pilot study

The influence of stress factors on selected phenotypic and genotypic features of Listeria monocytogenes – a pilot study

Antimicrobials and Food-Related Stresses as Selective Factors for Antibiotic Resistance along the Farm to Fork Continuum

Genomic and pathogenicity islands of Listeria monocytogenes—overview of selected aspects

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