Effect of environmental factors on performance of immobilized consortium system for degradation of carbendazim and 2,4-dichlorophenoxyacetic acid in continuous culture

Nagase, Hirotsugu; Pattanasupong, Anchana; Sugimoto, Eiko; Tani, Katsuji; Nasu, Masao; Hirata, Kazumasa; Miyamoto, Kazuhisa

doi:10.1016/j.bej.2005.03.017

Cited by 12 publications

(7 citation statements)

References 11 publications

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“…It is also possible that the preserved bacterial consortia might need more time to recover their methomyl-degrading ability; thus, we did not observe high removal rates within 7 days. In contrast, Nagas et al (2006) found that the degradation ability of an immobilized consortium for carbendazim and 2,4-dichlorophenoxyacetic acid could be stably maintained at least 6 months by simple preservation methods. Therefore, more studies are needed to obtain the best preservation technique for the methomyl-degrading consortia.…”

Section: Removal Of Methomyl By Acclimatized Bacterial Consortia On Lmentioning

confidence: 72%

“…Some environmental factors (e.g. pH, temperature and nutrients) have been found to affect the degradation activity of microorganisms (Pattanasupong et al 2004a;Nagas et al 2006). Therefore, further study of the optimum conditions for improving the degradation activity of isolated bacterial species is essential.…”

Section: Removal Of Methomyl By Isolated Bacterial Strainsmentioning

confidence: 99%

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The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

Chen

Wang

et al. 2014

Int. J. Environ. Sci. Technol.

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Complex microbial communities from river biofilms might contain microorganisms capable of degrading xenobiotic pollutants such as pesticides (e.g. methomyl, which is commonly detected in rivers). Therefore, this study was used to determine the methomyl degradation potential of bacteria consortia and single bacterial strains acclimatized and isolated from natural river biofilms to provide biomaterials for bioremediation of water that is contaminated with methomyl. Natural river biofilms were culture enriched with methomyl as the sole carbon source to obtain acclimatized bacterial consortia and single bacterial strains. The microbial consortium on the ceramic discs was able to remove 91 % of added methomyl (50 mg l -1 ) in 7 days. The longer-acclimatized bacterial consortium on loofah sponges removed methomyl more quickly than the shorter-acclimatized consortium, but both had similar removal capabilities (i.e. 92.4 and 92.2 %). This finding suggested that the former might contain more methomyl degraders than the latter. However, after preservation at 25, 4 and -20°C for 1 or 3 months, the methomyl degradation ability of the bacterial consortia decreased significantly, indicating loss of methomyl degraders during preservation. Three bacterial species were isolated from acclimatized river biofilms, and only one species, identified as Sphingomonas sp., was able to remove methomyl, with a 7-day removal rate of 44.7 % when sugar was added and of 32.5 % when no sugar was added. These results suggested that an additional carbon source might slightly improve the ability of Sphingomonas sp. to degrade methomyl. Acclimatized bacterial consortia have a higher potential for treating methomyl-contaminated water than isolated bacterial species.

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Section: Removal Of Methomyl By Acclimatized Bacterial Consortia On Lmentioning

confidence: 72%

Section: Removal Of Methomyl By Isolated Bacterial Strainsmentioning

confidence: 99%

The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

Chen

Wang

et al. 2014

Int. J. Environ. Sci. Technol.

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“…A number of bacterial species are known to degrade benzimidazole structures and most of them were isolated from fungicide contaminated soils. Their potential for the degradation of various fungicides has been extensively studied both in liquid media and soils (Motonaga et al 1996;Ş ahin and Tamer 2000;Nagase et al 2006). Bacteria capable of degrading MBC have been isolated and characterized (Holtman and Kobayashi 1997;Zhang et al 2005); however there is no information on identified species that participate in the transformation of TM to MBC.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

2010

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Degradation of the fungicide thiophanate-methyl (TM) by Enterobacter sp. TDS-1 and Bacillus sp. TDS-2 isolated from sandy soil previously treated with TM was studied in mineral salt medium (MSM) and soil. Both strains were able to grow in MSM supplemented with TM (50 mg l(-1)) as the sole carbon source. Over a 16 days incubation period, 60 and 77% of the initial dose of TM were degraded by strains TDS-1 and TDS-2, respectively, and disappearance of TM was described by first-order kinetics. Medium supplementation with glucose markedly stimulated bacterial growth; while the final rate of TM degradation was reduced by 21 and 27% for strains TDS-1 and TDS-2, respectively as compared to medium with TM only. Moreover, this additional carbon source changed the TM degradation kinetics, which proceeded according to a zero-order model. This effect was linked to substrate competition and/or a strong decrease of medium pH. Isolates degraded TM (100 mg kg(-1)) in soil with rate constants of 0.186 and 0.210 day(-1), following first-order rate kinetics, and the time in which the initial TM concentration was reduced by 50% (DT50) in soils inoculated with strains TDS-1 and TDS-2 were 6.3 and 5.1 days, respectively. Analysis of TM degradation products in soil showed that the tested strains may have the potential to transform carbendazim (MBC) to 2-aminobenzimidazole (2-AB), and may be useful for a bioremediation of MBC-polluted soils.

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“…For example, Chen et al found that the methomyl degradation ability of bacterial consortia decreased after preserving at −20, 4, and 25°C for 1 and 3 months. No effect on degradation abilities of immobilized consortium to MBC and 2,4‐D after preserving at 4°C and room temperature up to 6 months was found by Nagase et al .…”

Section: Resultsmentioning

confidence: 87%

Accessing the Carbofuran Degradation Ability of Cultures From Natural River Biofilms in Different Environments

Tien

Huang

Chen

2017

CLEAN Soil Air Water

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Acclimatized microbial consortia and bacterial strains were isolated from natural river biofilms to investigate their carbofuran degradation ability. Under 28‐day continuous enrichment culturing of river biofilms, the acclimatized microbial consortia were obtained and showed a high carbofuran removal rate (52.5%). The batch experiments showed that 28‐ and 35‐day loofah‐sponge‐attached acclimatized consortia (i.e., microbial consortia A and B) had low carbofuran removal rates. However, after preservation, the carbofuran degradation abilities of both microbial consortia recovered possibly due to the growth of carbofuran degraders. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that Stenotrophomonas sp., Pseudomonas fulva, Comamonas jiangduensis, and Thermolithobacter sp. dominated in both microbial consortia. The best preservation conditions to have high carbofuran removal for microbial consortia A and B were −20°C for 3 months (55.7% removal) and 25°C for 1 month (73.9% removal), respectively. The isolated Sphingobacterium multivorum showed high carbofuran removal rates with and without sugar (67.4 and 73.1% removal) at pH 7 (25°C), followed by those at pH 7 (30°C) and pH 9 (25°C) (41.2 and 32.1% removal). This strain was able to degrade carbofuran in the simulated carbofuran‐contaminated paddy fields. The study demonstrated that the immobilized microbial consortia and S. multivorum from river biofilms are good biomaterials for bioremediating carbofuran‐contaminated sites.

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Effect of environmental factors on performance of immobilized consortium system for degradation of carbendazim and 2,4-dichlorophenoxyacetic acid in continuous culture

Cited by 12 publications

References 11 publications

The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

Accessing the Carbofuran Degradation Ability of Cultures From Natural River Biofilms in Different Environments

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