Effect of environmental and chemotactic stimuli on the activity of the Campylobacter jejuni flaA σ28 promoter

Allen, K

doi:10.1016/s0378-1097(01)00444-x

Cited by 17 publications

(21 citation statements)

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“…The flaA gene carries consensus promoter sequences for σ 28 and flaB has promoter sequences for σ 54 (13,14). Differential expression of two different flagellin genes enables bacteria to respond to different environmental conditions (2,3). In this study, we found that a luxS mutation affected flaA transcription but not flaB transcription (Fig.…”

Section: Discussionmentioning

confidence: 56%

Effects of Quorum Sensing on flaA Transcription and Autoagglutination in Campylobacter jejuni

Jeon

Itoh

Misawa

et al. 2003

Microbiology and Immunology

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Some bacteria can communicate with other species of bacteria by means of autoinducer‐2 (AI‐2)‐mediated quorum sensing. In this study, we demonstrated that AI‐2‐mediated quorum sensing influences the transcription of flaA, the major flagellin gene in Campylobacter jejuni. A null mutation of luxS in C. jejuni strain 81116 reduced flaA transcription (approximately 43% that of the wild‐type) and induced a reduction in motility. However, the luxS mutant had the same level of total flagellin protein as the wild‐type. Transmission electron microscopy showed that the flagellar structure was preserved in the luxS mutant. The agglutination capability was reduced in the mutant strain, implying that quorum sensing might be involved in the formation of surface structures of C. jejuni. These observations suggest that AI‐2‐mediated quorum sensing may play a role in regulation of motility and surface properties in C. jejuni.

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Section: Discussionmentioning

confidence: 56%

Effects of Quorum Sensing on flaA Transcription and Autoagglutination in Campylobacter jejuni

Jeon

Itoh

Misawa

et al. 2003

Microbiology and Immunology

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“…There was no difference in flaB-dependent CAT expression within this pH range. Recently, Allen & Griffiths (2001) described pH-dependent activities of the Campylobacter jejuni flaA promoter, measured by a luciferase reporter system. In their system, flaA transcriptional activity was moderately higher (about twice) at acidic compared to neutral pH.…”

Section: Influence Of Environmental Factors On Regulation Of Flagellamentioning

confidence: 99%

Growth phase-dependent and differential transcriptional control of flagellar genes in Helicobacter pylori

Niehus

Suerbaum

et al. 2002

Microbiology

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INTRODUCTIONThe eubacterial genus Helicobacter comprises the gastric Helicobacter species that infect the stomach mucosa of many different kinds of mammals. All of these have developed specific adaptation mechanisms to permit persistent life in the inhospitable environment of the stomach. Helicobacter pylori, the causative agent of chronic gastritis and peptic ulcers in humans, like other Helicobacter species depends on its strong motility to move through the viscous mucus gel lining the epithelium of the human stomach, to establish chronic infection of the human gastric mucosa and to persist in the gastric mucus (Hazell et al., 1986 ; Worku et al., 1999). Flagellar filaments contain FlaA and FlaB subunits in a stoichiometric ratio of approximately ten to one (Kostrzynska et al., 1991 ;Suerbaum et al., 1993 ;Josenhans et al., 1995aJosenhans et al., , 1999. Mutants lacking the major flagellin FlaA show strongly impaired motility in vitro and possess only truncated flagella, whereas flaB mutants have moderately diminished motility and apparently normal flagella. In the gnotobiotic piglet animal model and the H. mustelae-ferret model, isogenic mutants lacking either the FlaB or the FlaA subunits were both severely impaired in their ability to colonize (Andrutis et al., 1997 ;Eaton et al., 1996). Those results demonstrated the importance of both flagellins in vivo. The H. pylori flaA and flaB genes are unlinked on the chromosome and their transcription is regulated by two different promoters (Leying et al., 1992 ;Suerbaum et al., 1993). Expression of flaA is controlled by a σ#)-dependent promoter, a class of promoters responsible for the transcriptional activation of late flagellar genes in many flagellated bacteria (class 3 ;Aizawa, 2000 ;Josenhans et al., 2002). flaB and several other flagellar (Spohn & Scarlato, 1999). Dependency of a number of flagellar genes on the ' environmentally regulated ' σ&% factor has also been observed in Caulobacter crescentus, Vibrio cholerae and Campylobacter species, (Anderson et al., 1995 ;Jagannathan et al., 2001 ;Prouty et al., 2001). However, the evolutionary, environmental and functional basis for the importance of σ&% promoters in flagellar regulation in all these bacterial species is not well established.The genetic background and global regulation of flagellar motility and signal transduction in Helicobacter species have not been thoroughly investigated yet. The complete genome sequences of two H. pylori strains have confirmed that both strains possess most motility genes known from other bacteria, but that these are much less frequently organized in operons than in most other bacteria (Alm et al., 1999 ; Tomb et al., 1997). Based on homology searches, some genes known from other bacteria to be important in motility regulation have not been identified in H. pylori (Josenhans & Suerbaum, 2001 Labigne-Roussel et al., 1988).H. pylori strains were cultured on blood agar plates (Columbia agar base II, Oxoid), supplemented with 10 % sheep blood and the following antibiotics : v...

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“…As a commensal residing primarily in the mucosal layer of the intestines, C. jejuni demonstrates motility toward and binds to mucin (20,31,45). L-Fucose is a prominent component of eukaryotic glycoproteins and comprises 4 to 14% of the total oligosaccharide component of mucin (3). Specifically, fucose has been described as a chemoattractant for C. jejuni (20,45), increasing flaA promoter activity (3).…”

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confidence: 99%

“…L-Fucose is a prominent component of eukaryotic glycoproteins and comprises 4 to 14% of the total oligosaccharide component of mucin (3). Specifically, fucose has been described as a chemoattractant for C. jejuni (20,45), increasing flaA promoter activity (3). In vitro binding of C. jejuni to ␣1,2-fucosylated glycans has been demonstrated previously (11,42), and free fucose reduces adherence to intestinal cells (10), suggesting recognition of this oligosaccharide by the bacterium.…”

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confidence: 99%

Phenotypic and Genotypic Evidence for l -Fucose Utilization by Campylobacter jejuni

2011

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Campylobacter jejuni remains among the leading causes of bacterial food-borne illness. The current understanding of Campylobacter physiology suggests that it is asaccharolytic and is unable to catabolize exogenous carbohydrates. Contrary to this paradigm, we provide evidence for L-fucose utilization by C. jejuni. The fucose phenotype, shown in chemically defined medium, is strain specific and linked to an 11-open reading frame (ORF) plasticity region of the bacterial chromosome. By constructing a mutation in fucP (encoding a putative fucose permease), one of the genes in the plasticity region, we found that this locus is required for fucose utilization. Consistent with their function in fucose utilization, transcription of the genes in the locus is highly inducible by fucose. PCR screening revealed a broad distribution of this genetic locus in strains derived from various host species, and the presence of this locus was consistently associated with fucose utilization. Birds inoculated with the fucP mutant strain alone were colonized at a level comparable to that by the wild-type strain; however, in cocolonization experiments, the mutant was significantly outcompeted by the wild-type strain when birds were inoculated with a low dose (10 5 CFU per bird). This advantage was not observed when birds were inoculated at a higher inoculum dose (10 8 CFU per bird). These results demonstrated a previously undescribed substrate that supports growth of C. jejuni and identified the genetic locus associated with the utilization of this substrate. These findings substantially enhance our understanding of the metabolic repertoire of C. jejuni and the role of metabolic diversity in Campylobacter pathobiology.

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Effect of environmental and chemotactic stimuli on the activity of the Campylobacter jejuni flaA σ28 promoter

Cited by 17 publications

References 3 publications

Effects of Quorum Sensing on flaA Transcription and Autoagglutination in Campylobacter jejuni

Effects of Quorum Sensing on flaA Transcription and Autoagglutination in Campylobacter jejuni

Growth phase-dependent and differential transcriptional control of flagellar genes in Helicobacter pylori

Phenotypic and Genotypic Evidence for l -Fucose Utilization by Campylobacter jejuni

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