Effect of enucleation on the expression of c-Fos protein in the supraoptic nucleus of the Japanese monkey (Macaca fuscata)

Kazi, Jamil Ahsan; Mori, Satoshi; Gao, Hongzhi; Uehara, F; Nakagawa, Shiro

doi:10.1016/s0006-8993(02)03370-x

Cited by 7 publications

(9 citation statements)

References 21 publications

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“…After the enucleation of one eye, in addition to the supraoptic nucleus, as observed in our previous report [14], a large number of c-Fos protein-like immunoreactive neurons were found in the lateral habenular nucleus, spinal trigeminal nucleus and midbrain periaqueductal gray matter, but the time course of c-Fos protein expression in the lateral habenular nucleus was different from those of other areas.…”

Section: Resultssupporting

confidence: 79%

“…Furthermore, in our previous study we observed significantly increased numbers of cFos-positive vasopressinergic neurons in the supraoptic nucleus 1 h after eye enucleation, and as it is known, the supraoptic nucleus plays a key role in stress response [14]. However, the time course of c-Fos protein expression in the lateral habenular nucleus was different from those of other areas.…”

Section: Discussionmentioning

confidence: 79%

“…Serial 40-Ìm-thick sections were cut with a freezing microtome and immersed in PBS. The immunocytochemical detection of c-Fos protein was performed using the peroxidase-antiperoxidase (PAP) detection protocol [14]. To insure penetration of antibodies, the sections were preincubated in a solution containing 0.3% Triton X-100 in PBS after blocking endogenous peroxidase.…”

Section: Methodsmentioning

confidence: 99%

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Prolonged Expression of c-Fos Protein in the Lateral Habenular Nucleus of the Japanese Monkey <i>(Macaca fuscata)</i> after Eye Enucleation

Kazi¹,

Mori²,

Kuchiiwa³

et al. 2004

Neurosignals

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To elucidate the effect of traumatic stress on the lateral habenular nucleus, we investigated the time course of the expression of c-Fos protein in this nucleus of the Japanese monkey (Macaca fuscata) after enucleation of one eye using c-Fos protein immunocytochemistry. c-Fos protein-like immunoreactive neurons were significantly increased; the increase started 1 h after the enucleation and remained high for 3–9 h in the lateral habenular nucleus on both sides. These results suggest that the prolonged expression of c-Fos protein occurred in the lateral habenular nucleus after traumatic stress through multiple transsynaptic activations.

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Section: Resultssupporting

confidence: 79%

Section: Discussionmentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

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Prolonged Expression of c-Fos Protein in the Lateral Habenular Nucleus of the Japanese Monkey <i>(Macaca fuscata)</i> after Eye Enucleation

Kazi¹,

Mori²,

Kuchiiwa³

et al. 2004

Neurosignals

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“…Serial 40 lxm thick sections were cut with a freezing microtome and immersed in PBS. The immunocytochemical detection of c-Fos protein was performed using the peroxidase-antiperoxidase (PAP) detection protocol (Kazi et al 2002). To insure penetration of antibodies, the sections were pre-incubated in a solution containing 0.3% Triton X-100 in PBS after blocking endogenous peroxidase.…”

Section: Methodsmentioning

confidence: 99%

Gabapentin completely attenuated the acute morphine induced c-Fos expression in the rat striatum

Kazi

Gee

2007

J Mol Neurosci

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ProThyrotropin-releasing hormone (proTRH) is a prohormone widely distributed in many areas of the brain. After biosynthesis, proTRH is subjected to post-translational processing to generate TRH and seven non-TRH peptides. Among these non-TRH sequences, we found previously that preproTRH178-199 could regulate the secretion of prolactin in suckled rats by their pups. Dopamine (DA), the main regulator of prolactin secretion, is produced in dopaminergic tyrosine hydroxylase (TH)-positive neurons in the hypothalamic arcuate nucleus (ARC). In this study we investigated whether prolactin release during the estrous sexual cycle is regulated by preproTRH178-199 through its effect on DA neurons of the ARC. We observed that biotinylated preproTRH178-199 bound to neurons in the ARC; this was higher during proestrus than during diestrus. Binding of preproTRH178-199 to DA neurons was seen only during proestrus in the ARC. Using primary neuronal hypothalamic cultures we found that preproTRH178-199 peptide decreased TH levels in a dose-responsive manner, whereas intra-ARC administration of preproTRH178-199 induced a 20-fold increase in plasma prolactin levels. Together, these results suggest a potential role for preproTRH178-199 in regulating dopaminergic neurons involved in the inhibition of pituitary prolactin release.

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“…Serial 40-μm-thick sections were cut with a freezing microtome (CM1510S, Leica Microsystems, Wetzlar, Germany) and immersed in PBS. The immunocytochemical detection of c-Fos protein was performed using the peroxidase-antiperoxidase (PAP) detection protocol (Kazi et al 2002). To insure penetration of antibodies, the sections were pre-incubated in a solution containing 0.3% Triton X-100 in PBS after blocking endogenous peroxidase.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Effect of Gabapentin on c-Fos Expression in the CNS after Paw Surgery in Rats

Kazi

Gee

2007

J Mol Neurosci

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Gabapentin (neurontin), a GABA analogue anticonvulsant has proven to be effective in anti-nociceptive activity as well as for the treatment of anxiety. Gabapentin (GBP) is well tolerated and shows very favorable side effects profile: The exact molecular mechanism of action of GBP to block postoperative pain and stress is not known. Therefore, to identify the functional neuroanatomical target sites of GBP in post-surgery as well as its effect on postsurgical process, we examined the effects of GBP on c-Fos expression in the supraspinal part of the central nervous system in rats. Using a well-validated rat model of surgical pain, we studied the neuroanatomical functional target sites of gabapentin after paw surgery. The effect of GBP was examined by means of c-Fos immunohistochemistry. A single intraperitoneal injection (i.p.) of GBP (150 mg/kg) or saline (control) was administered 20 min before surgical incision in the paw under anesthesia. Ninety minutes after surgical incision, the deeply anesthetized rats were perfused transcardially with 4% paraformaldehyde. Serial 40-microm-thick sections of whole brain (except spinal cord) were cut and processed by immunohistochemistry to locate and quantify the sites and number of neurons with c-Fos immunoreactivity. Detection of c-Fos protein was performed using the peroxidase-antiperoxidase detection protocol. Our present study demonstrated that compared to control, administration of GBP (150 mg/kg, i.p.) before paw surgery significantly (p < 0.01) attenuated the incision-induced c-Fos expression only in the paraventricular nucleus of the hypothalamus. In addition, GBP-induced increase in c-Fos expression was observed in the dorsal raphe (DRN) and in the nucleus raphe magnus. Present results indicate that GBP may differentially modulate c-Fos expression in surgical paw incision. Moreover, this study provides some clue to examine whether GBP exerts its action simultaneously through two separate pathways in post-surgery.

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Effect of enucleation on the expression of c-Fos protein in the supraoptic nucleus of the Japanese monkey (Macaca fuscata)

Cited by 7 publications

References 21 publications

Prolonged Expression of c-Fos Protein in the Lateral Habenular Nucleus of the Japanese Monkey <i>(Macaca fuscata)</i> after Eye Enucleation

Prolonged Expression of c-Fos Protein in the Lateral Habenular Nucleus of the Japanese Monkey <i>(Macaca fuscata)</i> after Eye Enucleation

Gabapentin completely attenuated the acute morphine induced c-Fos expression in the rat striatum

Effect of Gabapentin on c-Fos Expression in the CNS after Paw Surgery in Rats

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