Effect of entomopathogenic nematode, Steinernema feltiae, on survival and plasma phenoloxidase activity of Helicoverpa armigera (Hb) (Lepidoptera: Noctuidae) in laboratory conditions

Ebrahimi, Laleh; Shiri, Mohammadreza; Dunphy, Gary B.

doi:10.1186/s41938-017-0016-x

Cited by 12 publications

(5 citation statements)

References 20 publications

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“…In addition, the cellular response of the insect hosts is highly different from one species to another (Alvandi et al, 2014;Rahatkhah et al, 2015;Lalitha et al, 2018). Also, the enzyme activity such as phenoloxidase and proteases may demonstrate such differences (Ibrahim et al, 2015;Ebrahimi et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Influence of Different Species of Steinernema and Heterorhabditis on Galleria mellonella and Spodoptera littoralis

Taha

2021

Egyptian Journal of Agronematology

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Entomopathogenic nematodes (EPNs) can be used efficiently as biological control agents against specific insects. Galleria mellonella (Gm) and Spodoptera littoralis (Sl) are considered important agricultural pests. The aim of this study was to evaluate the susceptibility of their larvae to ascending levels of four species of EPNs, Steinernema carpocapsae (Sc), S. arenarium (Sa), Heterorhabditis bacteriophora (Hb) and H. indica (Hi) as well as the ability of nematodes to penetrate and reproduce within their hosts. Fifth larval instar of Gm and Sl were used to evaluate the effect of four inoculum doses [200,400,800, and 1600 infective juveniles (IJs)] of each nematode species. Percentage of insect mortality (% M) and the nematode-rate of reproduction (Rr) were calculated after 24 and 48 hrs. The data confirmed that H. bacteriophora was the highest effective species on both insects. The % M was 75.7, 79.7, 82.3, and 83% after 24 hrs and 98.0, 99.3,100 and 100% after 48 hrs in Gm for these doses, respectively. On the other hand, the corresponding mortalities were 75.0, 78.3, 81.7, and 83.8% after 24 hrs and 97.3, 98.3, 100 and 100% after 48 hrs in Sl. The highest initial EPN population that could penetrate the insect (pi) was achieved by Hb in Gm (295 IJs) at 1600 IJs, while it was 221.7 IJs in Sl larvae at the same inoculum. The highest final population and Rr were obtained at the inoculum level of 200 and 400 IJs. in Gm and Sl, respectively. The study documented that Heterorhabditis and Steinernema can be used effectively in Gm and Sl management. Moreover, Hb is the most suppressive species tested against the two lepidopteran hosts.

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Section: Discussionmentioning

confidence: 99%

Influence of Different Species of Steinernema and Heterorhabditis on Galleria mellonella and Spodoptera littoralis

Taha

2021

Egyptian Journal of Agronematology

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“…Various genera and species of the EPNs have been isolated from Iran and investigated in different aspects such as morphology and phylogeny (Kary et al, 2009and Nikdel et al, 2010, natural incidence in insect hosts (Karimi et al, 2009), efficacy against agricultural pests (Ebrahimi et al, 2011(Ebrahimi et al, , 2014(Ebrahimi et al, , 2016, interaction of entomopathogenic nematodes and chemical pesticides (Sheykhnejad et al, 2014), and their interaction with insect immune system (Ebrahimi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

First report of the entomopathogenic nematode, Steinernema carpocapsae, from Moghan region of Iran and its efficacy against the turnip moth, Agrotis segetum Denis and Schiffermuller (Lepidoptera: Noctuidae), larvae

Ebrahimi¹,

Tanhamaafi²,

Sharifi³

2019

Egypt J Biol Pest Control

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During a survey of entomopathogenic nematodes (EPNs) in Moghan region of Iran in 2015, a steinernematid species was isolated, using the Galleria-baiting method. Based on its morphological and phylogenetic analysis of molecular data, the isolate was identified as Steinernema carpocapsae. The ITS rDNA sequence was deposited in NCBI (National Center for Biotechnology Information) with accession number of MF187616.1. Nucleotide row data was edited, using MEGA 6.0 software, and homologous sequences were involved in analysis, using Blast software. Sequences were aligned using Clustal W. Bootstrap analysis. The phylogenetic tree was constructed by the maximum likelihood method, using MEGA 6.0 software, and Steinernema feltiae was used as out-group. The turnip moth, Agrotis segetum Denis and Schiffermuller (Lepidoptera: Noctuidae), is one of the most important and destructive cutworm pests in Moghan. Therefore, the lethal effect of S. carpocapsae isolate Moghan (IRMoghan) was evaluated in a soil assay against the last instar larvae of A. segetum under laboratory conditions. The bioassay results showed high susceptibility of the larvae to S. carpocapsae. The LC 10 , LC 50 , and LC 90 values were 9.9, 54.13, and 246.2 IJs (infective juveniles) per larva of the pest, respectively (χ 2 = 7.36; df = 3, P value = 0.061). Reproduction of the EPNs within the dissected cadavers was observed. The bioassay results indicated that the new isolate is a promising biocontrol agent against A. segetum larvae with success recycling through them.

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“…The bio-efficacy of B. bassiana studied by Prasad et al [67] against H. armigera with four different doses sprayed topically against fourth-instar larvae recorded 76.7% mortality at the highest dose (0.25 mL × 10 8 spores/mL). Ebrahimi et al [68] studied the effect of the entomopathogenic nematode, Steinernema feltiae Filipjev (Rhabditida: Steinernematidae), on survival and plasma phenoloxidase activity of H. armigera in the lab. Another study conducted by Majeed et al [69] documented the pathogenicity of indigenous soil isolate of Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) on H. armigera.…”

Section: Discussionmentioning

confidence: 99%

Potential Use of Fusarium Isolates as Biological Control Agents: Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) Case Study

Mantzoukas

Kitsiou

Lagogiannis³

et al. 2022

Applied Sciences

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The cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) is a notorious agricultural pest with world-wide distribution, extreme polyphagy, high mobility and fecundity, facultative diapause, and significant resistance to chemical insecticides. Isolates from various Fusarium species were collected from soil, identified, and tested for their entomopathogenicity against H. armigera larvae in field experiments. Fungi of the genus Fusarium are ubiquitous and include phytopathogenic as well as entomopathogenic strains. Seven Fusarium species were identified and tested, including: F. algeriense, F. chlamydosporum var. chlamydosporum, F. fujikuroi, F. longifundum, F. pseudoanthophilum, F.solani, and F. tonkinense. All the collected fungi demonstrated a notable insecticidal effect on H. armigera larvae in field conditions, while some proved to be significantly lethal. The larval mortality of H. armigera ranged from 10 (103 conidia/mL) to 91% (108 conidia/mL) after 9 days (216 h). Larval survival time in treated plants ranged from 95 h (108 conidia/mL) to 208 h (103 conidia/mL). According to our results, F. solani isolate displayed the highest toxicity against H. armigera larvae and could be considered as a promising biocontrol agent of this serious pest.

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Effect of entomopathogenic nematode, Steinernema feltiae, on survival and plasma phenoloxidase activity of Helicoverpa armigera (Hb) (Lepidoptera: Noctuidae) in laboratory conditions

Cited by 12 publications

References 20 publications

Influence of Different Species of Steinernema and Heterorhabditis on Galleria mellonella and Spodoptera littoralis

Influence of Different Species of Steinernema and Heterorhabditis on Galleria mellonella and Spodoptera littoralis

First report of the entomopathogenic nematode, Steinernema carpocapsae, from Moghan region of Iran and its efficacy against the turnip moth, Agrotis segetum Denis and Schiffermuller (Lepidoptera: Noctuidae), larvae

Potential Use of Fusarium Isolates as Biological Control Agents: Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) Case Study

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