Effect of Enhydra fluctuans on Kidney Function in Alloxan-induced Diabetic Rats

Abstract

AIM: The aim of this study was to explore the effect of n-hexane fraction of Enhydra fluctuans aerial on kidney function in alloxan induced diabetic rats.
 METHODS: Five groups of diabetic Wistar rats were studied: Group 1 was given 0.5% Na-CMC (G0), group 2 was given glibenclamide 0.45 mg/kg (G1), groups 3, 4, and 5 were given a dose of n-hexane fraction 57.03, 114.06, and 171.09 mg/kg respectively. The experiment was completed in 21 days. Blood glucose was estimated on day 0 and day 21 of treatment. Histology of kidney, creatinine, and blood urea nitrogen (BUN) was examined. ANOVA was used to evaluate quantitative data, which was then followed by Duncan's new multiple range test (p < 0.05).
 RESULTS: Our results demonstrate that n-hexane fraction dosages of 57.03 mg/kg and 114,06 mg/kg significantly improved blood glucose profile, BUN, and creatinine in diabetic rats. Moreover, the dosage of 57.03 mg/kg is effective to counteract necrosis and fibrosis of kidney cells.
 
 CONCLUSION: Our findings revealed that the administration of the n-hexane fraction of E. fluctuans aerial improved the kidney function of diabetic rats, especially at the dosage of 57.03 mg/kg. Therefore, E. fluctuans can be relied upon to be a drug to prevent the development of diabetes mellitus and diabetic nephropathy.

Neurosteroid, Natural and Anabolic Steroids: Physiological, Immunological and Histopathological Study on Diabetic Albino Rats

“…The rats with alloxan-induced diabetes had various histopathological abnormalities in their kidney such as increased fibrosis, glomerular hypertrophy, glomerular space narrowing, loss of tubules brush border, and necrotic and thickened basal membranes. As well as, histological changes of liver with alloxan induction including congestion, necrosis, hepatic hyperplasia and acute hepatopathy [57,58]. Additionally, diabetes leads to several histological alterations in many organs, including the spleen.…”