Effect of energy source during culture on in vitro embryo development, resistance to cryopreservation and sex ratio

Rubessa, M.; Boccia, Lorenzo; Campanile, Giuseppe; Longobardi, V.; Albarella, Sara; Tateo, Alessandra; Zicarelli, L.; Gasparrini, B.

doi:10.1016/j.theriogenology.2011.06.004

Cited by 25 publications

(15 citation statements)

References 47 publications

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“…[7] reported that glucose supplementation immediately after fertilization at the concentration of 3 mM or less to modified SOF medium containing 5% superovulated cow serum did not affect blastocyst formation. However, there are some reports showing that addition of high concentration of glucose immediately after in vitro fertilization was harmful for early embryonic development [1, 6, 9, 13]. These findings are consistent with our results.…”

supporting

confidence: 93%

Improvement of Preimplantation Development of <i>In Vitro-</i>Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium

Sakagami¹,

Nishino²,

Adachi³

et al. 2014

The Journal of Veterinary Medical Science

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The influences of glucose supplementation on early development of bovine embryos in BSA-free synthetic oviduct fluid were examined. Among the groups supplemented with 1.5, 2.0, 4.0 or 5.6 mM glucose either at 0, 72 or 144 hr after fertilization, blastocysts yield significantly increased in the group supplemented with 4.0 mM glucose 144 hr after fertilization compared to the controls without glucose supplementation. The results suggest that appropriate amounts of glucose supplemented to the medium at the specific stage of embryo culture may be useful for the production of bovine blastocysts.

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supporting

confidence: 93%

Improvement of Preimplantation Development of <i>In Vitro-</i>Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium

Sakagami¹,

Nishino²,

Adachi³

et al. 2014

The Journal of Veterinary Medical Science

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“…Male and female preimplantation embryos differ not only in their chromosomal complement, but in their proteome and subsequent metabolome. Studies have reported [26] , [27] that embryos of different sex have different metabolic levels for amino acids and glucose. Furthermore, on Day 4 female embryos consumed 28% more glucose compared with males (P<0.05).…”

Section: Discussionmentioning

confidence: 99%

Probing the Effect of Human Normal Sperm Morphology Rate on Cycle Outcomes and Assisted Reproductive Methods Selection

Huang

et al. 2014

PLoS ONE

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Sperm morphology is the best predictor of fertilization potential, and the critical predictive information for supporting assisted reproductive methods selection. Given its important predictive value and the declining reality of semen quality in recent years, the threshold of normal sperm morphology rate (NSMR) is being constantly corrected and controversial, from the 4th edition (14%) to the 5th version (4%). We retrospectively analyzed 4756 cases of infertility patients treated with conventional-IVF(c-IVF) or ICSI, which were divided into three groups according to NSMR: ≥14%, 4%–14% and <4%. Here, we demonstrate that, with decrease in NSMR(≥14%, 4%–14%, <4%), in the c-IVF group, the rate of fertilization, normal fertilization, high-quality embryo, multi-pregnancy and birth weight of twins gradually decreased significantly (P<0.05), while the miscarriage rate was significantly increased (p<0.01) and implantation rate, clinical pregnancy rate, ectopic pregnancy rate, preterm birth rate, live birth rate, sex ratio, and birth weight(Singleton) showed no significant change. In the ICSI group, with decrease in NSMR (≥14%, 4%–14%, <4%), high-quality embryo rate, multi-pregnancy rate and birth weight of twins were gradually decreased significantly (p<0.05), while other parameters had no significant difference. Considering the clinical assisted methods selection, in the NFMR ≥14% group, normal fertilization rate of c-IVF was significantly higher than the ICSI group (P<0.05), in the 4%–14% group, birth weight (twins) of c-IVF were significantly higher than the ICSI group, in the <4% group, miscarriage of IVF was significantly higher than the ICSI group. Therefore, we conclude that NSMR is positively related to embryo reproductive potential, and when NSMR<4% (5th edition), ICSI should be considered first, while the NSMR≥4%, c-IVF assisted reproduction might be preferred.

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“…Experimental work supports the notion that glucose may favour males as a consequence of selective loss of female embryos in cattle (Gutierrez-Adan et al, 2001;Jimenez et al, 2003;Kimura et al, 2005;Rubessa et al, 2011) and mice (Machado et al, 2001;Jimenez et al, 2003). However, though glucose may be associated with the sex of human embryos, it is not clear that this is so as a result of selective female foetal loss (Gardner et al, 2011).…”

Section: Cameron's Hypothesismentioning

confidence: 92%

Hypotheses on the stability and variation of human sex ratios at birth

James

2012

Journal of Theoretical Biology

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Effect of energy source during culture on in vitro embryo development, resistance to cryopreservation and sex ratio

Cited by 25 publications

References 47 publications

Improvement of Preimplantation Development of <i>In Vitro-</i>Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium

Improvement of Preimplantation Development of <i>In Vitro-</i>Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium

Probing the Effect of Human Normal Sperm Morphology Rate on Cycle Outcomes and Assisted Reproductive Methods Selection

Hypotheses on the stability and variation of human sex ratios at birth

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