Effect of endothelin receptor antagonist bosentan on plasma leptin concentration in acute myocardial infarction in rats

Ostrowski, Robert P.; Januszewski, Sławomir; Kowalska, Z; Kapuściński, A

doi:10.1016/s0928-4680(03)00028-2

Cited by 8 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…injection of ketamine/acepromazine (50/5 mg/kg) the rat was euthanized. The heart was removed from the thoracic cavity and perfused retrograde through a cannula inserted in the ascending aorta with 6 ml pre-warmed saline followed by 6 ml 1.5% TTC solution [6]. The heart was then placed in a -80 °C freezer for 15 min.…”

Section: Triphenyltetrazolium Chloride (Ttc) Stainingmentioning

confidence: 99%

Quantitative imaging of myocardial infarct in rats with high resolution pinhole SPECT

Acton

Thomas

Zhou

2006

Int J Cardiovasc Imaging

View full text Add to dashboard Cite

Small animal imaging of cardiovascular disease using single photon emission tomography (SPECT) can be used to provide quantitative measurements of myocardial infarct. The purpose of this study was to demonstrate the accuracy of pinhole SPECT imaging with [ 99m Tc]sestamibi for estimation of infarct size in a rat model of coronary artery disease. Nine rats had their left anterior descending artery ligated to induce a region of myocardial infarct. These animals were injected with 37 MBq [ 99m Tc]sestamibi, and, 1 h later, scanned on a pinhole SPECT system for 30 min. The defect size measured with SPECT, which was dependent on a threshold applied to the short axis circumferential profiles, was compared against the gold standard triphenyltetrazolium chloride (TTC) staining. The size of the perfusion deficit measured using [ 99m Tc]sestamibi SPECT compared very favorably with the TTC staining result, for threshold values in the range 50-70%. The optimum threshold was approximately 70%, giving an excellent correlation (R 2 =0.89, p<0.001). Estimation of infarct size by [ 99m Tc] sestamibi SPECT yielded an excellent agreement with TTC staining. In conclusion, measurement of myocardial infarct with SPECT can be used to study the rat heart in vivo, and provides a quantitative measure of myocardial viability.

show abstract

Section: Triphenyltetrazolium Chloride (Ttc) Stainingmentioning

confidence: 99%

Quantitative imaging of myocardial infarct in rats with high resolution pinhole SPECT

Acton

Thomas

Zhou

2006

Int J Cardiovasc Imaging

View full text Add to dashboard Cite

show abstract

“…However, in addition to obesity, plasma leptin levels are also increased in several cardiac pathologies. For example, plasma leptin is increased in experimental and clinical myocardial infarction (16,19). It has been shown that plasma leptin concentrations may represent a predictor of first myocardial infarction (21) and that leptin may represent an independent risk factor for the development of ischemic heart disease (23).…”

Section: Rat Heart Is a Site Of Leptin Production And Actionmentioning

confidence: 99%

Rat heart is a site of leptin production and action

Purdham¹,

Zou²,

Rajapurohitam³

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

146

116

View full text Add to dashboard Cite

Leptin, the 16-kDa peptide hormone product of the ob gene, is produced primarily by adipocytes and was initially thought to exert its effects exclusively through actions on the hypothalamus via distinct leptin receptors termed OB-R. However, recent data show that leptin is produced elsewhere and that receptors are present in many other tissues. Using real-time PCR, we determined whether leptin and its receptors are present in the rat heart and demonstrated regional distribution patterns and gender differences as well as the effect of ischemia and reperfusion. Gene expression of leptin and its receptors (OB-Ra, OB-Rb, and OB-Re) was identified in myocytes and whole heart homogenates from all regions of the heart of male and female rats, with the highest abundance in left and right atria of male and female rats, respectively. No differences in regional distribution of OB-R were evident in male rat hearts. In female rats, expression was highest in right atria for all three isoforms and was significantly greater than in male rats. Ischemia and reperfusion significantly downregulated leptin and OB-R expression, although this was more pronounced in male rat hearts. Leptin release in the coronary effluent was also detected using ELISA, although this was generally unaffected by global ischemia and reperfusion. Our results demonstrate for the first time the presence of the leptin system, including the peptide and its receptors, in all regions of the rat heart. In view of emerging evidence for cardiac effects of leptin, it is proposed that the heart is a target for leptin action and that the peptide modulates function through a paracrine- or autocrine-dependent manner.

show abstract

“…The TTC staining followed a previously described protocol (22). The heart was subsequently frozen for 2 days to allow for radioactive decay.…”

Section: Methodsmentioning

confidence: 99%

Noninvasive assessment of myocardial viability in a small animal model: Comparison of MRI, SPECT, and PET

Thomas

Bal

Arkles

et al. 2008

Magnetic Resonance in Med

View full text Add to dashboard Cite

Acute myocardial infarction (AMI) research relies increasingly on small animal models and noninvasive imaging methods such as MRI, single-photon emission computed tomography (SPECT), and positron emission tomography (PET). However, a direct comparison among these techniques for characterization of perfusion, viability, and infarct size is lacking. Rats were studied within 18 -24 hr post AMI by MRI (4.7 T) and subsequently (40 -48 hr post AMI) by SPECT ( 99 Tc-MIBI) and micro-PET ( 18 FDG). A necrosis-specific MRI contrast agent was used to detect AMI, and a fast low angle shot (FLASH) sequence was used to acquire late enhancement and functional images contemporaneously. Infarcted regions showed late enhancement, whereas corresponding radionuclide images had reduced tracer uptake. MRI most accurately depicted AMI, showing the closest correlation and agreement with triphenyl tetrazolium chloride (TTC), followed by SPECT and PET. In some animals a mismatch of reduced uptake in normal myocardium and relatively increased 18

show abstract

Effect of endothelin receptor antagonist bosentan on plasma leptin concentration in acute myocardial infarction in rats

Cited by 8 publications

References 52 publications

Quantitative imaging of myocardial infarct in rats with high resolution pinhole SPECT

Quantitative imaging of myocardial infarct in rats with high resolution pinhole SPECT

Rat heart is a site of leptin production and action

Noninvasive assessment of myocardial viability in a small animal model: Comparison of MRI, SPECT, and PET

Contact Info

Product

Resources

About