Effect of Endophytic Fungal Associations on the Chemical Profile of in vitro Vochysia divergens Seedlings

Abstract

Vochysia divergens (Vochysiaceae) is considered an invasive species in the wetlands of the Brazilian Pantanal, which hinders the cultivation of agricultural species. In this study, we evaluated the chemical profile by HPLC-DAD (high-performance liquid chromatography-diode array detector) of leaves extracts from V. divergens seedlings inoculated with endophytic fungi isolated from V. divergens roots. These fungi were collected on dry (D) and wet (W) seasons in the Pantanal. The presence of tannin hexahydroxydiphenoyl (HHDP)-galloyl-glucose and flavone 3',5'-dimethoxy-luteolin were predominant in the seedlings inoculated with endophytic fungi W experiments at 100 and 80%, respectively. Likewise, flavone 3',5-dimethoxy-luteolin-7-O-β-glucoside showed a similar representation in the two evaluated periods, compared with 5-methoxy-luteolin, which was detected only in seedlings inoculated with W endophytic fungi. This approach is new to V. divergens, which has no scientific data on its in vitro elicitation, in the search for a better understanding of the ecological relationships of this species.

Bioprospecting and Structure of Fungal Endophyte Communities Found in the Brazilian Biomes, Pantanal, and Cerrado

“…In order to associate economic value with biodiversity, bioprospecting studies have reported the biotechnological potential of endophytes against a large number of pathogens (Schulz et al, 2002 ; Gathage et al, 2016 ; Larran et al, 2016 ; Li et al, 2016 ; Hokama et al, 2017 ; Parpinelli et al, 2017 ). In this context, we investigated the antimicrobial potential of the endophytes of V. divergens and S. adstringens .…”

“…Studies in the Pantanal and Cerrado are usually on animal and plant biodiversity (Hyde and Soytong, 2008 ; Porras-Alfaro and Bayman, 2011 ). A very limited number of studies in these biomes are conducted on endophytic microorganisms, and most of them are focused on aspects of bioprospecting without community analysis (Carvalho et al, 2012 ; Glienke et al, 2012 ; Vieira et al, 2014 ; Savi et al, 2016 ; Gos et al, 2017 ; Hokama et al, 2017 ; Parpinelli et al, 2017 ; Sousa et al, 2017 ).…”

Chemical composition and in vitro cytotoxicity of Corynespora olivacea (V18) associated with Vochysia divergens / Composição química e citotoxicidade in vitro de Corynespora olivacea (V18) associada a Vochysia divergens

“…Because the association of V. divergens with endophytic microorganisms is poorly understood, and since the chemical profile and biological potential of its microbiota remains partially unknown, our research group has been dedicated to the chemical study of this plant species (Pimenta et al, 2015) and its chemical interaction with its endophytic microbiota (Parpinelli et al, 2017;Pimenta et al, 2017;de Oliveira et al, 2018;Farias et al, 2019).…”